Exploring the Relationship between the Inhibition Selectivity and the Apoptosis of Roscovitine-Treated Cancer Cells
The antitumor activity of roscovitine was tested in four cervical carcinoma cells: C33A, HCE-1, HeLa, and SiHa. The effects of roscovitine on ATP Lite assay, cell cycle, and apoptosis were assessed. The Sub-G1 DNA content occurred great increasing, and this indicates that apoptosis was induced quick...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2013/389390 |
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| _version_ | 1832552244501807104 |
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| author | Chunying Cui Yaonan Wang Yuji Wang Ming Zhao Shiqi Peng |
| author_facet | Chunying Cui Yaonan Wang Yuji Wang Ming Zhao Shiqi Peng |
| author_sort | Chunying Cui |
| collection | DOAJ |
| description | The antitumor activity of roscovitine was tested in four cervical carcinoma cells: C33A, HCE-1, HeLa, and SiHa. The effects of roscovitine on ATP Lite assay, cell cycle, and apoptosis were assessed. The Sub-G1 DNA content occurred great increasing, and this indicates that apoptosis was induced quickly in HeLa cells, but slowly in the other cells. The morphological observation results showed that roscovitine induced apoptosis and cell death in the cervical carcinoma cells. Results revealed that roscovitine exhibited selective cytotoxicity towards 4 cervical carcinoma cells, and the cells showed different morphologic and apoptotic changes at the same concentration. It was estimated that cervical carcinoma cells responded differently to roscovitine because of differences in apoptotic and genetic background in different cervical carcinoma cells. This study suggested that roscovitine had the potential to be a chemotherapeutic agent against cervical carcinoma. |
| format | Article |
| id | doaj-art-abe1bf119ba04192a95a987b35b60402 |
| institution | Kabale University |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-abe1bf119ba04192a95a987b35b604022025-02-03T05:59:14ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732013-01-01201310.1155/2013/389390389390Exploring the Relationship between the Inhibition Selectivity and the Apoptosis of Roscovitine-Treated Cancer CellsChunying Cui0Yaonan Wang1Yuji Wang2Ming Zhao3Shiqi Peng4College of Pharmaceutical Sciences, Capital Medical University, Beijing 100069, ChinaMedical Experiment and Test Center, Capital Medical University, Beijing 100069, ChinaCollege of Pharmaceutical Sciences, Capital Medical University, Beijing 100069, ChinaCollege of Pharmaceutical Sciences, Capital Medical University, Beijing 100069, ChinaCollege of Pharmaceutical Sciences, Capital Medical University, Beijing 100069, ChinaThe antitumor activity of roscovitine was tested in four cervical carcinoma cells: C33A, HCE-1, HeLa, and SiHa. The effects of roscovitine on ATP Lite assay, cell cycle, and apoptosis were assessed. The Sub-G1 DNA content occurred great increasing, and this indicates that apoptosis was induced quickly in HeLa cells, but slowly in the other cells. The morphological observation results showed that roscovitine induced apoptosis and cell death in the cervical carcinoma cells. Results revealed that roscovitine exhibited selective cytotoxicity towards 4 cervical carcinoma cells, and the cells showed different morphologic and apoptotic changes at the same concentration. It was estimated that cervical carcinoma cells responded differently to roscovitine because of differences in apoptotic and genetic background in different cervical carcinoma cells. This study suggested that roscovitine had the potential to be a chemotherapeutic agent against cervical carcinoma.http://dx.doi.org/10.1155/2013/389390 |
| spellingShingle | Chunying Cui Yaonan Wang Yuji Wang Ming Zhao Shiqi Peng Exploring the Relationship between the Inhibition Selectivity and the Apoptosis of Roscovitine-Treated Cancer Cells Journal of Analytical Methods in Chemistry |
| title | Exploring the Relationship between the Inhibition Selectivity and the Apoptosis of Roscovitine-Treated Cancer Cells |
| title_full | Exploring the Relationship between the Inhibition Selectivity and the Apoptosis of Roscovitine-Treated Cancer Cells |
| title_fullStr | Exploring the Relationship between the Inhibition Selectivity and the Apoptosis of Roscovitine-Treated Cancer Cells |
| title_full_unstemmed | Exploring the Relationship between the Inhibition Selectivity and the Apoptosis of Roscovitine-Treated Cancer Cells |
| title_short | Exploring the Relationship between the Inhibition Selectivity and the Apoptosis of Roscovitine-Treated Cancer Cells |
| title_sort | exploring the relationship between the inhibition selectivity and the apoptosis of roscovitine treated cancer cells |
| url | http://dx.doi.org/10.1155/2013/389390 |
| work_keys_str_mv | AT chunyingcui exploringtherelationshipbetweentheinhibitionselectivityandtheapoptosisofroscovitinetreatedcancercells AT yaonanwang exploringtherelationshipbetweentheinhibitionselectivityandtheapoptosisofroscovitinetreatedcancercells AT yujiwang exploringtherelationshipbetweentheinhibitionselectivityandtheapoptosisofroscovitinetreatedcancercells AT mingzhao exploringtherelationshipbetweentheinhibitionselectivityandtheapoptosisofroscovitinetreatedcancercells AT shiqipeng exploringtherelationshipbetweentheinhibitionselectivityandtheapoptosisofroscovitinetreatedcancercells |