Enhancement of Anti-Inflammatory and Osteogenic Abilities of Mesenchymal Stem Cells via Cell-to-Cell Adhesion to Periodontal Ligament-Derived Fibroblasts
Mesenchymal stem cells (MSCs) are involved in anti-inflammatory events and tissue repair; these functions are activated by their migration or homing to inflammatory tissues in response to various chemokines. However, the mechanism by which MSCs interact with other cell types in inflammatory tissue r...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2017-01-01
|
| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2017/3296498 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832561657917734912 |
|---|---|
| author | Keita Suzuki Naoyuki Chosa Shunsuke Sawada Naoki Takizawa Takashi Yaegashi Akira Ishisaki |
| author_facet | Keita Suzuki Naoyuki Chosa Shunsuke Sawada Naoki Takizawa Takashi Yaegashi Akira Ishisaki |
| author_sort | Keita Suzuki |
| collection | DOAJ |
| description | Mesenchymal stem cells (MSCs) are involved in anti-inflammatory events and tissue repair; these functions are activated by their migration or homing to inflammatory tissues in response to various chemokines. However, the mechanism by which MSCs interact with other cell types in inflammatory tissue remains unclear. We investigated the role of periodontal ligament fibroblasts (PDL-Fs) in regulating the anti-inflammatory and osteogenic abilities of bone marrow-derived- (BM-) MSCs. The expression of monocyte chemotactic protein- (MCP-)1 was significantly enhanced by stimulation of PDL-Fs with inflammatory cytokines. MCP-1 induced the migratory ability of BM-MSCs but not PDL-Fs. Expression levels of anti-inflammatory and inflammatory cytokines were increased and decreased, respectively, by direct-contact coculture between MSCs and PDL-Fs. In addition, the direct-contact coculture enhanced the expression of MSC markers that play important roles in the self-renewal and maintenance of multipotency of MSCs, which in turn induced the osteogenic ability of the cells. These results suggest that MCP-1 induces the migration and homing of BM-MSCs into the PDL inflammatory tissue. The subsequent adherence of MSCs to PDL-Fs plays an immunomodulatory role to terminate inflammation during wound healing and upregulates the expression stem cell markers to enhance the stemness of MSCs, thereby facilitating bone formation in damaged PDL tissue. |
| format | Article |
| id | doaj-art-abb94e5e476f48cfab1e2ddb67130d52 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-abb94e5e476f48cfab1e2ddb67130d522025-02-03T01:24:31ZengWileyStem Cells International1687-966X1687-96782017-01-01201710.1155/2017/32964983296498Enhancement of Anti-Inflammatory and Osteogenic Abilities of Mesenchymal Stem Cells via Cell-to-Cell Adhesion to Periodontal Ligament-Derived FibroblastsKeita Suzuki0Naoyuki Chosa1Shunsuke Sawada2Naoki Takizawa3Takashi Yaegashi4Akira Ishisaki5Division of Cellular Biosignal Sciences, Department of Biochemistry, Iwate Medical University, Yahaba, Iwate 028-3694, JapanDivision of Cellular Biosignal Sciences, Department of Biochemistry, Iwate Medical University, Yahaba, Iwate 028-3694, JapanDivision of Periodontology, Department of Conservative Dentistry, Iwate Medical University School of Dentistry, Morioka, Iwate 020-8505, JapanDivision of Periodontology, Department of Conservative Dentistry, Iwate Medical University School of Dentistry, Morioka, Iwate 020-8505, JapanDivision of Periodontology, Department of Conservative Dentistry, Iwate Medical University School of Dentistry, Morioka, Iwate 020-8505, JapanDivision of Cellular Biosignal Sciences, Department of Biochemistry, Iwate Medical University, Yahaba, Iwate 028-3694, JapanMesenchymal stem cells (MSCs) are involved in anti-inflammatory events and tissue repair; these functions are activated by their migration or homing to inflammatory tissues in response to various chemokines. However, the mechanism by which MSCs interact with other cell types in inflammatory tissue remains unclear. We investigated the role of periodontal ligament fibroblasts (PDL-Fs) in regulating the anti-inflammatory and osteogenic abilities of bone marrow-derived- (BM-) MSCs. The expression of monocyte chemotactic protein- (MCP-)1 was significantly enhanced by stimulation of PDL-Fs with inflammatory cytokines. MCP-1 induced the migratory ability of BM-MSCs but not PDL-Fs. Expression levels of anti-inflammatory and inflammatory cytokines were increased and decreased, respectively, by direct-contact coculture between MSCs and PDL-Fs. In addition, the direct-contact coculture enhanced the expression of MSC markers that play important roles in the self-renewal and maintenance of multipotency of MSCs, which in turn induced the osteogenic ability of the cells. These results suggest that MCP-1 induces the migration and homing of BM-MSCs into the PDL inflammatory tissue. The subsequent adherence of MSCs to PDL-Fs plays an immunomodulatory role to terminate inflammation during wound healing and upregulates the expression stem cell markers to enhance the stemness of MSCs, thereby facilitating bone formation in damaged PDL tissue.http://dx.doi.org/10.1155/2017/3296498 |
| spellingShingle | Keita Suzuki Naoyuki Chosa Shunsuke Sawada Naoki Takizawa Takashi Yaegashi Akira Ishisaki Enhancement of Anti-Inflammatory and Osteogenic Abilities of Mesenchymal Stem Cells via Cell-to-Cell Adhesion to Periodontal Ligament-Derived Fibroblasts Stem Cells International |
| title | Enhancement of Anti-Inflammatory and Osteogenic Abilities of Mesenchymal Stem Cells via Cell-to-Cell Adhesion to Periodontal Ligament-Derived Fibroblasts |
| title_full | Enhancement of Anti-Inflammatory and Osteogenic Abilities of Mesenchymal Stem Cells via Cell-to-Cell Adhesion to Periodontal Ligament-Derived Fibroblasts |
| title_fullStr | Enhancement of Anti-Inflammatory and Osteogenic Abilities of Mesenchymal Stem Cells via Cell-to-Cell Adhesion to Periodontal Ligament-Derived Fibroblasts |
| title_full_unstemmed | Enhancement of Anti-Inflammatory and Osteogenic Abilities of Mesenchymal Stem Cells via Cell-to-Cell Adhesion to Periodontal Ligament-Derived Fibroblasts |
| title_short | Enhancement of Anti-Inflammatory and Osteogenic Abilities of Mesenchymal Stem Cells via Cell-to-Cell Adhesion to Periodontal Ligament-Derived Fibroblasts |
| title_sort | enhancement of anti inflammatory and osteogenic abilities of mesenchymal stem cells via cell to cell adhesion to periodontal ligament derived fibroblasts |
| url | http://dx.doi.org/10.1155/2017/3296498 |
| work_keys_str_mv | AT keitasuzuki enhancementofantiinflammatoryandosteogenicabilitiesofmesenchymalstemcellsviacelltocelladhesiontoperiodontalligamentderivedfibroblasts AT naoyukichosa enhancementofantiinflammatoryandosteogenicabilitiesofmesenchymalstemcellsviacelltocelladhesiontoperiodontalligamentderivedfibroblasts AT shunsukesawada enhancementofantiinflammatoryandosteogenicabilitiesofmesenchymalstemcellsviacelltocelladhesiontoperiodontalligamentderivedfibroblasts AT naokitakizawa enhancementofantiinflammatoryandosteogenicabilitiesofmesenchymalstemcellsviacelltocelladhesiontoperiodontalligamentderivedfibroblasts AT takashiyaegashi enhancementofantiinflammatoryandosteogenicabilitiesofmesenchymalstemcellsviacelltocelladhesiontoperiodontalligamentderivedfibroblasts AT akiraishisaki enhancementofantiinflammatoryandosteogenicabilitiesofmesenchymalstemcellsviacelltocelladhesiontoperiodontalligamentderivedfibroblasts |