Comparison of Disk Diffusion and Etest Methods to Determine the Susceptibility of Staphylococcus aureus Circulating in Riyadh, Saudi Arabia to Fusidic Acid
Fusidic acid is a common therapy for staphylococcal infections in Saudi Arabia, but reports have suggested high rates of resistance among clinical isolates. Susceptibility testing of S. aureus to fusidic acid is further complicated by the lack of consensus on mean inhibitory concentrations (MIC) and...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2012/391251 |
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| author | Ali M. Somily David R. Peaper Elijah Paintsil Thomas S. Murray |
| author_facet | Ali M. Somily David R. Peaper Elijah Paintsil Thomas S. Murray |
| author_sort | Ali M. Somily |
| collection | DOAJ |
| description | Fusidic acid is a common therapy for staphylococcal infections in Saudi Arabia, but reports have suggested high rates of resistance among clinical isolates. Susceptibility testing of S. aureus to fusidic acid is further complicated by the lack of consensus on mean inhibitory concentrations (MIC) and disk diffusion cutoffs to determine resistance. The purpose of this study was to determine the correlation between disk diffusion and Etest determined MIC susceptibility results in clinical isolates of S. aureus from a large academic hospital in Riyadh, Saudi Arabia. Our data demonstrate excellent correlation between Etest determined MIC and disk diffusion susceptibility data, using either previously proposed zone sizes of ≥21 mm as susceptible and ≤18 mm as resistant or the EUCAST recommended zone size of ≤24 mm for resistance, in an area with relatively high rates of fusidic acid resistance. |
| format | Article |
| id | doaj-art-aba2bf12aea344b3aa46b246d6ad9904 |
| institution | Kabale University |
| issn | 1687-918X 1687-9198 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj-art-aba2bf12aea344b3aa46b246d6ad99042025-02-03T01:31:19ZengWileyInternational Journal of Microbiology1687-918X1687-91982012-01-01201210.1155/2012/391251391251Comparison of Disk Diffusion and Etest Methods to Determine the Susceptibility of Staphylococcus aureus Circulating in Riyadh, Saudi Arabia to Fusidic AcidAli M. Somily0David R. Peaper1Elijah Paintsil2Thomas S. Murray3Department of Pathology, Microbiology Unit, College of Medicine, King Saud University and King Khalid University Hospital, Riyadh 11411, Saudi ArabiaDepartment of Laboratory Medicine, Yale School of Medicine, New Haven, CT 06510, USADepartments of Pediatrics and Pharmacology, Yale School of Medicine, New Haven, CT 06510, USADepartment of Laboratory Medicine, Yale School of Medicine, New Haven, CT 06510, USAFusidic acid is a common therapy for staphylococcal infections in Saudi Arabia, but reports have suggested high rates of resistance among clinical isolates. Susceptibility testing of S. aureus to fusidic acid is further complicated by the lack of consensus on mean inhibitory concentrations (MIC) and disk diffusion cutoffs to determine resistance. The purpose of this study was to determine the correlation between disk diffusion and Etest determined MIC susceptibility results in clinical isolates of S. aureus from a large academic hospital in Riyadh, Saudi Arabia. Our data demonstrate excellent correlation between Etest determined MIC and disk diffusion susceptibility data, using either previously proposed zone sizes of ≥21 mm as susceptible and ≤18 mm as resistant or the EUCAST recommended zone size of ≤24 mm for resistance, in an area with relatively high rates of fusidic acid resistance.http://dx.doi.org/10.1155/2012/391251 |
| spellingShingle | Ali M. Somily David R. Peaper Elijah Paintsil Thomas S. Murray Comparison of Disk Diffusion and Etest Methods to Determine the Susceptibility of Staphylococcus aureus Circulating in Riyadh, Saudi Arabia to Fusidic Acid International Journal of Microbiology |
| title | Comparison of Disk Diffusion and Etest Methods to Determine the Susceptibility of Staphylococcus aureus Circulating in Riyadh, Saudi Arabia to Fusidic Acid |
| title_full | Comparison of Disk Diffusion and Etest Methods to Determine the Susceptibility of Staphylococcus aureus Circulating in Riyadh, Saudi Arabia to Fusidic Acid |
| title_fullStr | Comparison of Disk Diffusion and Etest Methods to Determine the Susceptibility of Staphylococcus aureus Circulating in Riyadh, Saudi Arabia to Fusidic Acid |
| title_full_unstemmed | Comparison of Disk Diffusion and Etest Methods to Determine the Susceptibility of Staphylococcus aureus Circulating in Riyadh, Saudi Arabia to Fusidic Acid |
| title_short | Comparison of Disk Diffusion and Etest Methods to Determine the Susceptibility of Staphylococcus aureus Circulating in Riyadh, Saudi Arabia to Fusidic Acid |
| title_sort | comparison of disk diffusion and etest methods to determine the susceptibility of staphylococcus aureus circulating in riyadh saudi arabia to fusidic acid |
| url | http://dx.doi.org/10.1155/2012/391251 |
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