Comparison of β-Propiolactone and Formalin Inactivation on Antigenicity and Immune Response of West Nile Virus
West Nile Virus (WNV) is a pathogenic arbovirus that belongs to genus Flavivirus under family Flaviviridae. Till now there are no approved vaccines against WNV for human use. In this study, the effect of two alkylating agents, formaldehyde and β-PL, generally used for inactivated vaccine preparation...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Advances in Virology |
| Online Access: | http://dx.doi.org/10.1155/2015/616898 |
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| author | Pritom Chowdhury Rashmee Topno Siraj A. Khan Jagadish Mahanta |
| author_facet | Pritom Chowdhury Rashmee Topno Siraj A. Khan Jagadish Mahanta |
| author_sort | Pritom Chowdhury |
| collection | DOAJ |
| description | West Nile Virus (WNV) is a pathogenic arbovirus that belongs to genus Flavivirus under family Flaviviridae. Till now there are no approved vaccines against WNV for human use. In this study, the effect of two alkylating agents, formaldehyde and β-PL, generally used for inactivated vaccine preparation, was assessed on the basis of antigenic and immunogenic potential of the inactivated WNV. Lineage 5 WNV isolates were inactivated by both formalin and β-PL treatments. Inactivation was confirmed by repeated passage in BHK-21 cell line and infant mice. Viruses inactivated by both the treatments showed higher antigenicity. Immune response in mice model showed serum anti-WNV antibody titre was moderately higher in formalin inactivated antigen compared to β-PL inactivated antigen. However, no significant differences were observed in neutralization antibody titre. In conclusion, we can state that both formaldehyde and β-PL inactivation processes were found to be equally efficient for inactivation of WNV. However, they need to be compared with other inactivating agents along with study on cell mediated immune response. |
| format | Article |
| id | doaj-art-ab9d69f6ee6d4f7aa87987e7ae8fbafe |
| institution | Kabale University |
| issn | 1687-8639 1687-8647 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advances in Virology |
| spelling | doaj-art-ab9d69f6ee6d4f7aa87987e7ae8fbafe2025-02-03T01:31:19ZengWileyAdvances in Virology1687-86391687-86472015-01-01201510.1155/2015/616898616898Comparison of β-Propiolactone and Formalin Inactivation on Antigenicity and Immune Response of West Nile VirusPritom Chowdhury0Rashmee Topno1Siraj A. Khan2Jagadish Mahanta3Arbovirology Group, Entomology and Filariasis Division, Regional Medical Research Centre, ICMR, Northeast Region, Post Box No. 105, Dibrugarh, Assam 786001, IndiaArbovirology Group, Entomology and Filariasis Division, Regional Medical Research Centre, ICMR, Northeast Region, Post Box No. 105, Dibrugarh, Assam 786001, IndiaArbovirology Group, Entomology and Filariasis Division, Regional Medical Research Centre, ICMR, Northeast Region, Post Box No. 105, Dibrugarh, Assam 786001, IndiaArbovirology Group, Entomology and Filariasis Division, Regional Medical Research Centre, ICMR, Northeast Region, Post Box No. 105, Dibrugarh, Assam 786001, IndiaWest Nile Virus (WNV) is a pathogenic arbovirus that belongs to genus Flavivirus under family Flaviviridae. Till now there are no approved vaccines against WNV for human use. In this study, the effect of two alkylating agents, formaldehyde and β-PL, generally used for inactivated vaccine preparation, was assessed on the basis of antigenic and immunogenic potential of the inactivated WNV. Lineage 5 WNV isolates were inactivated by both formalin and β-PL treatments. Inactivation was confirmed by repeated passage in BHK-21 cell line and infant mice. Viruses inactivated by both the treatments showed higher antigenicity. Immune response in mice model showed serum anti-WNV antibody titre was moderately higher in formalin inactivated antigen compared to β-PL inactivated antigen. However, no significant differences were observed in neutralization antibody titre. In conclusion, we can state that both formaldehyde and β-PL inactivation processes were found to be equally efficient for inactivation of WNV. However, they need to be compared with other inactivating agents along with study on cell mediated immune response.http://dx.doi.org/10.1155/2015/616898 |
| spellingShingle | Pritom Chowdhury Rashmee Topno Siraj A. Khan Jagadish Mahanta Comparison of β-Propiolactone and Formalin Inactivation on Antigenicity and Immune Response of West Nile Virus Advances in Virology |
| title | Comparison of β-Propiolactone and Formalin Inactivation on Antigenicity and Immune Response of West Nile Virus |
| title_full | Comparison of β-Propiolactone and Formalin Inactivation on Antigenicity and Immune Response of West Nile Virus |
| title_fullStr | Comparison of β-Propiolactone and Formalin Inactivation on Antigenicity and Immune Response of West Nile Virus |
| title_full_unstemmed | Comparison of β-Propiolactone and Formalin Inactivation on Antigenicity and Immune Response of West Nile Virus |
| title_short | Comparison of β-Propiolactone and Formalin Inactivation on Antigenicity and Immune Response of West Nile Virus |
| title_sort | comparison of β propiolactone and formalin inactivation on antigenicity and immune response of west nile virus |
| url | http://dx.doi.org/10.1155/2015/616898 |
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