Suppression of porcine polyspermy using mechanical vibrations during in vitro fertilization
The efficiency of porcine in vitro fertilized (IVF) embryo production remains low. Polyspermy is considered a contributing factor to this result. In this study, we investigated the effects of mechanical vibrations during the co-culture of oocytes and spermatozoa on fertilization parameters and subse...
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| Language: | English |
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The Society for Reproduction and Development
2025-02-01
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| Series: | The Journal of Reproduction and Development |
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| Online Access: | https://www.jstage.jst.go.jp/article/jrd/71/2/71_2024-042/_pdf/-char/en |
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| author | Takehiro HIMAKI Kohei SHINADA Asumi YAEGASHI |
| author_facet | Takehiro HIMAKI Kohei SHINADA Asumi YAEGASHI |
| author_sort | Takehiro HIMAKI |
| collection | DOAJ |
| description | The efficiency of porcine in vitro fertilized (IVF) embryo production remains low. Polyspermy is considered a contributing factor to this result. In this study, we investigated the effects of mechanical vibrations during the co-culture of oocytes and spermatozoa on fertilization parameters and subsequent embryonic development. The rate of polyspermy decreased significantly in all vibration culture groups compared with the stationary culture (control) group (P < 0.05). Regarding subsequent embryonic development, the blastocyst formation rate was significantly improved in the middle-vibration culture group compared with the control group (P < 0.05). However, the high-vibration culture group had the lowest sperm penetration rate and did not show any improvement in monospermy rate and normal fertilization efficiency. In addition, their in vitro developmental status was the lowest. These results indicate that moderate mechanical vibrations during insemination effectively suppress polyspermy and improve porcine IVF embryo production efficiency. |
| format | Article |
| id | doaj-art-ab69b6f4929e4ee592064f13a3460bd5 |
| institution | OA Journals |
| issn | 0916-8818 1348-4400 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | The Society for Reproduction and Development |
| record_format | Article |
| series | The Journal of Reproduction and Development |
| spelling | doaj-art-ab69b6f4929e4ee592064f13a3460bd52025-08-20T02:16:05ZengThe Society for Reproduction and DevelopmentThe Journal of Reproduction and Development0916-88181348-44002025-02-0171211011410.1262/jrd.2024-042jrdSuppression of porcine polyspermy using mechanical vibrations during in vitro fertilizationTakehiro HIMAKI0Kohei SHINADA1Asumi YAEGASHI2Department of Agricultural and Environmental Science, Faculty of Applied Biological Sciences, Gifu University, Gifu 501-1193, JapanGraduate School of Natural Science and Technology, Gifu University, Gifu 501-1193, JapanDepartment of Agricultural and Environmental Science, Faculty of Applied Biological Sciences, Gifu University, Gifu 501-1193, JapanThe efficiency of porcine in vitro fertilized (IVF) embryo production remains low. Polyspermy is considered a contributing factor to this result. In this study, we investigated the effects of mechanical vibrations during the co-culture of oocytes and spermatozoa on fertilization parameters and subsequent embryonic development. The rate of polyspermy decreased significantly in all vibration culture groups compared with the stationary culture (control) group (P < 0.05). Regarding subsequent embryonic development, the blastocyst formation rate was significantly improved in the middle-vibration culture group compared with the control group (P < 0.05). However, the high-vibration culture group had the lowest sperm penetration rate and did not show any improvement in monospermy rate and normal fertilization efficiency. In addition, their in vitro developmental status was the lowest. These results indicate that moderate mechanical vibrations during insemination effectively suppress polyspermy and improve porcine IVF embryo production efficiency.https://www.jstage.jst.go.jp/article/jrd/71/2/71_2024-042/_pdf/-char/enin vitro developmentin vitro fertilizationmechanical vibrationpolyspermyporcine |
| spellingShingle | Takehiro HIMAKI Kohei SHINADA Asumi YAEGASHI Suppression of porcine polyspermy using mechanical vibrations during in vitro fertilization The Journal of Reproduction and Development in vitro development in vitro fertilization mechanical vibration polyspermy porcine |
| title | Suppression of porcine polyspermy using mechanical vibrations during in vitro fertilization |
| title_full | Suppression of porcine polyspermy using mechanical vibrations during in vitro fertilization |
| title_fullStr | Suppression of porcine polyspermy using mechanical vibrations during in vitro fertilization |
| title_full_unstemmed | Suppression of porcine polyspermy using mechanical vibrations during in vitro fertilization |
| title_short | Suppression of porcine polyspermy using mechanical vibrations during in vitro fertilization |
| title_sort | suppression of porcine polyspermy using mechanical vibrations during in vitro fertilization |
| topic | in vitro development in vitro fertilization mechanical vibration polyspermy porcine |
| url | https://www.jstage.jst.go.jp/article/jrd/71/2/71_2024-042/_pdf/-char/en |
| work_keys_str_mv | AT takehirohimaki suppressionofporcinepolyspermyusingmechanicalvibrationsduringinvitrofertilization AT koheishinada suppressionofporcinepolyspermyusingmechanicalvibrationsduringinvitrofertilization AT asumiyaegashi suppressionofporcinepolyspermyusingmechanicalvibrationsduringinvitrofertilization |