Osteogenesis from Dental Pulp Derived Stem Cells: A Novel Conditioned Medium Including Melatonin within a Mixture of Hyaluronic, Butyric, and Retinoic Acids
Human dental pulp stem cells (hDPSCs) have shown relevant potential for cell therapy in the orthopedic and odontoiatric fields. The optimization of their osteogenic potential is currently a major challenge. Vascular endothelial growth factor A (VEGF A) has been recently reported to act as a major co...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2016/2056416 |
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| author | Margherita Maioli Valentina Basoli Sara Santaniello Sara Cruciani Alessandro Palmerio Delitala Roberto Pinna Egle Milia Regina Grillari-Voglauer Vania Fontani Salvatore Rinaldi Roberta Muggironi Gianfranco Pigliaru Carlo Ventura |
| author_facet | Margherita Maioli Valentina Basoli Sara Santaniello Sara Cruciani Alessandro Palmerio Delitala Roberto Pinna Egle Milia Regina Grillari-Voglauer Vania Fontani Salvatore Rinaldi Roberta Muggironi Gianfranco Pigliaru Carlo Ventura |
| author_sort | Margherita Maioli |
| collection | DOAJ |
| description | Human dental pulp stem cells (hDPSCs) have shown relevant potential for cell therapy in the orthopedic and odontoiatric fields. The optimization of their osteogenic potential is currently a major challenge. Vascular endothelial growth factor A (VEGF A) has been recently reported to act as a major conductor of osteogenesis in vitro and in vivo. Here, we attempted to prime endogenous VEGF A expression without the need for viral vector mediated gene transfer technologies. We show that hDPSCs exposure to a mixture of hyaluronic, butyric, and retinoic acids (HA + BU + RA) induced the transcription of a gene program of osteogenesis and the acquirement of an osteogenic lineage. Such response was also elicited by cell exposure to melatonin, a pleiotropic agent that recently emerged as a remarkable osteogenic inducer. Interestingly, the commitment to the osteogenic fate was synergistically enhanced by the combinatorial exposure to a conditioned medium containing both melatonin and HA + BU + RA. These in vitro results suggest that in vivo osteogenesis might be improved and further studies are needed. |
| format | Article |
| id | doaj-art-ab2d82c104344eb5bbab81000afd0ce8 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-ab2d82c104344eb5bbab81000afd0ce82025-02-03T01:11:16ZengWileyStem Cells International1687-966X1687-96782016-01-01201610.1155/2016/20564162056416Osteogenesis from Dental Pulp Derived Stem Cells: A Novel Conditioned Medium Including Melatonin within a Mixture of Hyaluronic, Butyric, and Retinoic AcidsMargherita Maioli0Valentina Basoli1Sara Santaniello2Sara Cruciani3Alessandro Palmerio Delitala4Roberto Pinna5Egle Milia6Regina Grillari-Voglauer7Vania Fontani8Salvatore Rinaldi9Roberta Muggironi10Gianfranco Pigliaru11Carlo Ventura12Department of Biomedical Sciences, University of Sassari, Viale San Pietro 43/B, 07100 Sassari, ItalyDepartment of Biomedical Sciences, University of Sassari, Viale San Pietro 43/B, 07100 Sassari, ItalyDepartment of Biomedical Sciences, University of Sassari, Viale San Pietro 43/B, 07100 Sassari, ItalyDepartment of Biomedical Sciences, University of Sassari, Viale San Pietro 43/B, 07100 Sassari, ItalyAzienda Ospedaliera Universitaria di Sassari, Via Michele Coppino 26, 07100 Sassari, ItalyDepartment of Biomedical Sciences, University of Sassari, Viale San Pietro 43/B, 07100 Sassari, ItalyDepartment of Biomedical Sciences, University of Sassari, Viale San Pietro 43/B, 07100 Sassari, ItalyDepartment of Biotechnology, University of Natural Resources and Life Sciences Vienna, Muthgasse 18, A-1190 Vienna, AustriaResearch Department, Rinaldi Fontani Foundation, Viale Belfiore 43, 50144 Florence, ItalyResearch Department, Rinaldi Fontani Foundation, Viale Belfiore 43, 50144 Florence, ItalyDepartment of Biomedical Sciences, University of Sassari, Viale San Pietro 43/B, 07100 Sassari, ItalyDepartment of Biomedical Sciences, University of Sassari, Viale San Pietro 43/B, 07100 Sassari, ItalyLaboratory of Molecular Biology and Stem Cell Engineering, National Institute of Biostructures and Biosystems, Via Massarenti, 40138 Bologna, ItalyHuman dental pulp stem cells (hDPSCs) have shown relevant potential for cell therapy in the orthopedic and odontoiatric fields. The optimization of their osteogenic potential is currently a major challenge. Vascular endothelial growth factor A (VEGF A) has been recently reported to act as a major conductor of osteogenesis in vitro and in vivo. Here, we attempted to prime endogenous VEGF A expression without the need for viral vector mediated gene transfer technologies. We show that hDPSCs exposure to a mixture of hyaluronic, butyric, and retinoic acids (HA + BU + RA) induced the transcription of a gene program of osteogenesis and the acquirement of an osteogenic lineage. Such response was also elicited by cell exposure to melatonin, a pleiotropic agent that recently emerged as a remarkable osteogenic inducer. Interestingly, the commitment to the osteogenic fate was synergistically enhanced by the combinatorial exposure to a conditioned medium containing both melatonin and HA + BU + RA. These in vitro results suggest that in vivo osteogenesis might be improved and further studies are needed.http://dx.doi.org/10.1155/2016/2056416 |
| spellingShingle | Margherita Maioli Valentina Basoli Sara Santaniello Sara Cruciani Alessandro Palmerio Delitala Roberto Pinna Egle Milia Regina Grillari-Voglauer Vania Fontani Salvatore Rinaldi Roberta Muggironi Gianfranco Pigliaru Carlo Ventura Osteogenesis from Dental Pulp Derived Stem Cells: A Novel Conditioned Medium Including Melatonin within a Mixture of Hyaluronic, Butyric, and Retinoic Acids Stem Cells International |
| title | Osteogenesis from Dental Pulp Derived Stem Cells: A Novel Conditioned Medium Including Melatonin within a Mixture of Hyaluronic, Butyric, and Retinoic Acids |
| title_full | Osteogenesis from Dental Pulp Derived Stem Cells: A Novel Conditioned Medium Including Melatonin within a Mixture of Hyaluronic, Butyric, and Retinoic Acids |
| title_fullStr | Osteogenesis from Dental Pulp Derived Stem Cells: A Novel Conditioned Medium Including Melatonin within a Mixture of Hyaluronic, Butyric, and Retinoic Acids |
| title_full_unstemmed | Osteogenesis from Dental Pulp Derived Stem Cells: A Novel Conditioned Medium Including Melatonin within a Mixture of Hyaluronic, Butyric, and Retinoic Acids |
| title_short | Osteogenesis from Dental Pulp Derived Stem Cells: A Novel Conditioned Medium Including Melatonin within a Mixture of Hyaluronic, Butyric, and Retinoic Acids |
| title_sort | osteogenesis from dental pulp derived stem cells a novel conditioned medium including melatonin within a mixture of hyaluronic butyric and retinoic acids |
| url | http://dx.doi.org/10.1155/2016/2056416 |
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