The formation and the study of a collection of the Miscanthus resource species gene pool in the conditions of the West Siberian forest steppe
Several species of the genus Miscanthus Anderss. (elephant grass) characterized by a high rate of growth of the aboveground vegetative mass are currently in the focus of attention due to their high practical application as a source of bioethanol and cellulose. The main goals of this study were: (1)...
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| Format: | Article |
| Language: | English |
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Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
2019-11-01
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| Series: | Вавиловский журнал генетики и селекции |
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| Online Access: | https://vavilov.elpub.ru/jour/article/view/2346 |
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| author | O. V. Dorogina O. Yu. Vasilyeva N. S. Nuzhdina I. V. Buglova E. V. Zhmud G. A. Zueva O. V. Kominа I. S. Kuban A. S. Gusar R. V. Dudkin |
| author_facet | O. V. Dorogina O. Yu. Vasilyeva N. S. Nuzhdina I. V. Buglova E. V. Zhmud G. A. Zueva O. V. Kominа I. S. Kuban A. S. Gusar R. V. Dudkin |
| author_sort | O. V. Dorogina |
| collection | DOAJ |
| description | Several species of the genus Miscanthus Anderss. (elephant grass) characterized by a high rate of growth of the aboveground vegetative mass are currently in the focus of attention due to their high practical application as a source of bioethanol and cellulose. The main goals of this study were: (1) molecular genetic identification and (2) histochemical analysis of the genus Miscanthus Anderss. species in the collection of Central Siberian Botanical Garden SB RAS in order to identify the most perspective and technically valuable individuals. To study the collection of Miscanthus samples, a multi-disciplinary approach was applied. To collect the samples of different species from native habitats, traditional systematic and geobotanical methods (comparative morphological and phytocenological) were used. According to the results of the ISSR-analysis, 16 samples of three Miscanthus species were divided into two clades: Sinensis and Sacchariflorus, the former including two subclades. For the samples of M. purpurascens_I and II, a hybrid origin of this species was confirmed by ISSR data. The molecular data obtained from the study allowed us to hypothesize that the samples involved in the subclade I of the Sinensis clade could be used as donors of resistance to adverse environments, and the samples of the subclade II, as donors of high biomass productivity. Based on histochemical analysis, sclerenchyma cells were characterized by the most lignin-rich thickened membranes, so the most appropriate direction in Miscanthus selection should be based on identification and using less lignin-containing samples. |
| format | Article |
| id | doaj-art-ab2cfcfdabe64d6696ee7da9c6e8d6e5 |
| institution | Kabale University |
| issn | 2500-3259 |
| language | English |
| publishDate | 2019-11-01 |
| publisher | Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders |
| record_format | Article |
| series | Вавиловский журнал генетики и селекции |
| spelling | doaj-art-ab2cfcfdabe64d6696ee7da9c6e8d6e52025-02-01T09:58:08ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592019-11-0123792693210.18699/VJ19.568991The formation and the study of a collection of the Miscanthus resource species gene pool in the conditions of the West Siberian forest steppeO. V. Dorogina0O. Yu. Vasilyeva1N. S. Nuzhdina2I. V. Buglova3E. V. Zhmud4G. A. Zueva5O. V. Kominа6I. S. Kuban7A. S. Gusar8R. V. Dudkin9Central Siberian Botanical Garden, SB RASCentral Siberian Botanical Garden, SB RASCentral Siberian Botanical Garden, SB RASCentral Siberian Botanical Garden, SB RASCentral Siberian Botanical Garden, SB RASCentral Siberian Botanical Garden, SB RASCentral Siberian Botanical Garden, SB RASCentral Siberian Botanical Garden, SB RASNovosibirsk State Agrarian UniversityBotanical Garden-Institute, FEB RASSeveral species of the genus Miscanthus Anderss. (elephant grass) characterized by a high rate of growth of the aboveground vegetative mass are currently in the focus of attention due to their high practical application as a source of bioethanol and cellulose. The main goals of this study were: (1) molecular genetic identification and (2) histochemical analysis of the genus Miscanthus Anderss. species in the collection of Central Siberian Botanical Garden SB RAS in order to identify the most perspective and technically valuable individuals. To study the collection of Miscanthus samples, a multi-disciplinary approach was applied. To collect the samples of different species from native habitats, traditional systematic and geobotanical methods (comparative morphological and phytocenological) were used. According to the results of the ISSR-analysis, 16 samples of three Miscanthus species were divided into two clades: Sinensis and Sacchariflorus, the former including two subclades. For the samples of M. purpurascens_I and II, a hybrid origin of this species was confirmed by ISSR data. The molecular data obtained from the study allowed us to hypothesize that the samples involved in the subclade I of the Sinensis clade could be used as donors of resistance to adverse environments, and the samples of the subclade II, as donors of high biomass productivity. Based on histochemical analysis, sclerenchyma cells were characterized by the most lignin-rich thickened membranes, so the most appropriate direction in Miscanthus selection should be based on identification and using less lignin-containing samples.https://vavilov.elpub.ru/jour/article/view/2346miscanthusissr analysishistochemical analysisbiomorphologymicroecologycluster dendrogrambioethanol |
| spellingShingle | O. V. Dorogina O. Yu. Vasilyeva N. S. Nuzhdina I. V. Buglova E. V. Zhmud G. A. Zueva O. V. Kominа I. S. Kuban A. S. Gusar R. V. Dudkin The formation and the study of a collection of the Miscanthus resource species gene pool in the conditions of the West Siberian forest steppe Вавиловский журнал генетики и селекции miscanthus issr analysis histochemical analysis biomorphology microecology cluster dendrogram bioethanol |
| title | The formation and the study of a collection of the Miscanthus resource species gene pool in the conditions of the West Siberian forest steppe |
| title_full | The formation and the study of a collection of the Miscanthus resource species gene pool in the conditions of the West Siberian forest steppe |
| title_fullStr | The formation and the study of a collection of the Miscanthus resource species gene pool in the conditions of the West Siberian forest steppe |
| title_full_unstemmed | The formation and the study of a collection of the Miscanthus resource species gene pool in the conditions of the West Siberian forest steppe |
| title_short | The formation and the study of a collection of the Miscanthus resource species gene pool in the conditions of the West Siberian forest steppe |
| title_sort | formation and the study of a collection of the miscanthus resource species gene pool in the conditions of the west siberian forest steppe |
| topic | miscanthus issr analysis histochemical analysis biomorphology microecology cluster dendrogram bioethanol |
| url | https://vavilov.elpub.ru/jour/article/view/2346 |
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