Structural Characterizations and Biological Evaluation of a Natural Polysaccharide from Branches of <i>Camellia oleifera</i> Abel
Background: <i>Camellia oleifera</i> Abel (<i>C. oleifera</i>) is widely cultivated and serves as an important source of edible oil. Yet, during oil production, pruned branches generate significant waste and contribute to environmental pollution. Objectives: In this work, we...
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2025-01-01
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| author | Shengjia Lu Yali Zhang Yanghui Ou Jianghui Xin Hongliang Yao Litao Guan |
| author_facet | Shengjia Lu Yali Zhang Yanghui Ou Jianghui Xin Hongliang Yao Litao Guan |
| author_sort | Shengjia Lu |
| collection | DOAJ |
| description | Background: <i>Camellia oleifera</i> Abel (<i>C. oleifera</i>) is widely cultivated and serves as an important source of edible oil. Yet, during oil production, pruned branches generate significant waste and contribute to environmental pollution. Objectives: In this work, we obtain a natural polysaccharide from the branches of <i>C. oleifera</i> and optimize its extraction using Box–Behnken design (BBD), which is a statistical method commonly used in response surface methodology. Additionally, we study its properties, such as monosaccharide composition, structural features, antioxidant, and anti-inflammatory abilities. Results: BBD was employed to optimize polysaccharide extraction (solid-liquid ratio = 1:40; 90 °C; 130 min) for a higher yield. After purification, the major monosaccharides of branches of <i>C. oleifera’s</i> polysaccharide (CBP) were disclosed as glucose and galactose. Subsequent structural features of CBP were measured. The antioxidant and anti-inflammatory abilities were measured. The highly scavenging rates of the 2,2-diphenyl-1-picrylhydrazyl and hydroxyl radicals, with the chelating capacity of Fe<sup>2+</sup>, indicate potent antioxidant activity of CBP. Conclusions: In general, CBP demonstrated significant anti-inflammatory activity with down-regulating the expression of IL-6 and IL-1β in the LPS-induced macrophage RAW264.7 model. This bioactive polysaccharide adds value to waste branches by offering a novel approach to waste recycling and the development of <i>C. oleifera</i>. |
| format | Article |
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| institution | Kabale University |
| issn | 1424-8247 |
| language | English |
| publishDate | 2025-01-01 |
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| series | Pharmaceuticals |
| spelling | doaj-art-ab249db3158c4cd683e5f6e830385e7a2025-01-24T13:45:11ZengMDPI AGPharmaceuticals1424-82472025-01-011815110.3390/ph18010051Structural Characterizations and Biological Evaluation of a Natural Polysaccharide from Branches of <i>Camellia oleifera</i> AbelShengjia Lu0Yali Zhang1Yanghui Ou2Jianghui Xin3Hongliang Yao4Litao Guan5College of Materials and Energy, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Key Laboratory of Animal Conservation and Resource Utilization, Institute of Zoology, Guangdong Academy of Sciences, Guangzhou 510260, ChinaGuangdong Key Laboratory of Animal Conservation and Resource Utilization, Institute of Zoology, Guangdong Academy of Sciences, Guangzhou 510260, ChinaCollege of Materials and Energy, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Key Laboratory of Animal Conservation and Resource Utilization, Institute of Zoology, Guangdong Academy of Sciences, Guangzhou 510260, ChinaCollege of Materials and Energy, South China Agricultural University, Guangzhou 510642, ChinaBackground: <i>Camellia oleifera</i> Abel (<i>C. oleifera</i>) is widely cultivated and serves as an important source of edible oil. Yet, during oil production, pruned branches generate significant waste and contribute to environmental pollution. Objectives: In this work, we obtain a natural polysaccharide from the branches of <i>C. oleifera</i> and optimize its extraction using Box–Behnken design (BBD), which is a statistical method commonly used in response surface methodology. Additionally, we study its properties, such as monosaccharide composition, structural features, antioxidant, and anti-inflammatory abilities. Results: BBD was employed to optimize polysaccharide extraction (solid-liquid ratio = 1:40; 90 °C; 130 min) for a higher yield. After purification, the major monosaccharides of branches of <i>C. oleifera’s</i> polysaccharide (CBP) were disclosed as glucose and galactose. Subsequent structural features of CBP were measured. The antioxidant and anti-inflammatory abilities were measured. The highly scavenging rates of the 2,2-diphenyl-1-picrylhydrazyl and hydroxyl radicals, with the chelating capacity of Fe<sup>2+</sup>, indicate potent antioxidant activity of CBP. Conclusions: In general, CBP demonstrated significant anti-inflammatory activity with down-regulating the expression of IL-6 and IL-1β in the LPS-induced macrophage RAW264.7 model. This bioactive polysaccharide adds value to waste branches by offering a novel approach to waste recycling and the development of <i>C. oleifera</i>.https://www.mdpi.com/1424-8247/18/1/51anti-inflammationfood healthpolysaccharide<i>Camellia oleifera</i> Abel |
| spellingShingle | Shengjia Lu Yali Zhang Yanghui Ou Jianghui Xin Hongliang Yao Litao Guan Structural Characterizations and Biological Evaluation of a Natural Polysaccharide from Branches of <i>Camellia oleifera</i> Abel Pharmaceuticals anti-inflammation food health polysaccharide <i>Camellia oleifera</i> Abel |
| title | Structural Characterizations and Biological Evaluation of a Natural Polysaccharide from Branches of <i>Camellia oleifera</i> Abel |
| title_full | Structural Characterizations and Biological Evaluation of a Natural Polysaccharide from Branches of <i>Camellia oleifera</i> Abel |
| title_fullStr | Structural Characterizations and Biological Evaluation of a Natural Polysaccharide from Branches of <i>Camellia oleifera</i> Abel |
| title_full_unstemmed | Structural Characterizations and Biological Evaluation of a Natural Polysaccharide from Branches of <i>Camellia oleifera</i> Abel |
| title_short | Structural Characterizations and Biological Evaluation of a Natural Polysaccharide from Branches of <i>Camellia oleifera</i> Abel |
| title_sort | structural characterizations and biological evaluation of a natural polysaccharide from branches of i camellia oleifera i abel |
| topic | anti-inflammation food health polysaccharide <i>Camellia oleifera</i> Abel |
| url | https://www.mdpi.com/1424-8247/18/1/51 |
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