Arginine–NO pathway modulated energy metabolism in the hepatopancreas and muscle of female Litopenaeus vannamei

Currently, the regulational effect of arginine on the energy metabolism is unknown in broodstock shrimp nutrition. Accordingly, an eight-week feeding trial was conducted to confirm it in female Litopenaeus vannamei. Six diets were formulated to contain graded levels of arginine (2.90 %, 3.58 %, 4.08...

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Main Authors: Haitao Fan, Xin Zhang, Yanan Yin, Lefei Jiao
Format: Article
Language:English
Published: Elsevier 2025-03-01
Series:Aquaculture Reports
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Online Access:http://www.sciencedirect.com/science/article/pii/S2352513424006252
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author Haitao Fan
Xin Zhang
Yanan Yin
Lefei Jiao
author_facet Haitao Fan
Xin Zhang
Yanan Yin
Lefei Jiao
author_sort Haitao Fan
collection DOAJ
description Currently, the regulational effect of arginine on the energy metabolism is unknown in broodstock shrimp nutrition. Accordingly, an eight-week feeding trial was conducted to confirm it in female Litopenaeus vannamei. Six diets were formulated to contain graded levels of arginine (2.90 %, 3.58 %, 4.08 %, 4.53 %, 5.04 % and 5.55 %). A total of 540 shrimp (with an initial weight of approximately 14 g) were allocated at random to six treatments, each of which consisted of three tanks with 30 shrimp each. The results showed that with the rise in the level of dietary arginine supplementation, growth performance showed an increasing trend, reaching the highest value in the 5.55 % arginine group. The gene expression of cellular energy sensor AMP-activated protein kinase was up-regulated in muscle and hepatopancreas. Dietary arginine supplementation decreased hemolymph glucose content and hepatopancreas crude lipid content, increased muscle crude lipid content, glycogen content in muscle and hepatopancreas. Dietary arginine increased muscle crude protein content, decreased hepatopancreas crude protein content and activated the target of rapamycin 1 pathway. Above results were further confirmed by gene expression. In addition, shrimp were injected with dsGFP and dsNOS for 48 h and successfully constructed the RNA interference model. The findings showed that the levels of nos, cgmp, ampk (a, b, c), genes involved in glucose metabolism, lipid metabolism and amino acid metabolism were inhibited. In the conclusion, arginine supplementation could promote growth performance through modulating energy metabolism. Arginine–NO pathway modulated energy metabolism in hepatopancreas and muscle to promote tissue glycogen synthesis, muscle protein and lipid deposition in female L. vannamei.
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spelling doaj-art-ab1d115539f9450088e075bce27f20e42025-02-06T05:12:03ZengElsevierAquaculture Reports2352-51342025-03-0140102537Arginine–NO pathway modulated energy metabolism in the hepatopancreas and muscle of female Litopenaeus vannameiHaitao Fan0Xin Zhang1Yanan Yin2Lefei Jiao3School of Marine Sciences, Ningbo University, Ningbo, Zhejiang 315211, ChinaSchool of Marine Sciences, Ningbo University, Ningbo, Zhejiang 315211, ChinaSchool of Marine Sciences, Ningbo University, Ningbo, Zhejiang 315211, ChinaCorresponding author.; School of Marine Sciences, Ningbo University, Ningbo, Zhejiang 315211, ChinaCurrently, the regulational effect of arginine on the energy metabolism is unknown in broodstock shrimp nutrition. Accordingly, an eight-week feeding trial was conducted to confirm it in female Litopenaeus vannamei. Six diets were formulated to contain graded levels of arginine (2.90 %, 3.58 %, 4.08 %, 4.53 %, 5.04 % and 5.55 %). A total of 540 shrimp (with an initial weight of approximately 14 g) were allocated at random to six treatments, each of which consisted of three tanks with 30 shrimp each. The results showed that with the rise in the level of dietary arginine supplementation, growth performance showed an increasing trend, reaching the highest value in the 5.55 % arginine group. The gene expression of cellular energy sensor AMP-activated protein kinase was up-regulated in muscle and hepatopancreas. Dietary arginine supplementation decreased hemolymph glucose content and hepatopancreas crude lipid content, increased muscle crude lipid content, glycogen content in muscle and hepatopancreas. Dietary arginine increased muscle crude protein content, decreased hepatopancreas crude protein content and activated the target of rapamycin 1 pathway. Above results were further confirmed by gene expression. In addition, shrimp were injected with dsGFP and dsNOS for 48 h and successfully constructed the RNA interference model. The findings showed that the levels of nos, cgmp, ampk (a, b, c), genes involved in glucose metabolism, lipid metabolism and amino acid metabolism were inhibited. In the conclusion, arginine supplementation could promote growth performance through modulating energy metabolism. Arginine–NO pathway modulated energy metabolism in hepatopancreas and muscle to promote tissue glycogen synthesis, muscle protein and lipid deposition in female L. vannamei.http://www.sciencedirect.com/science/article/pii/S2352513424006252ArginineEnergy metabolismFemale Litopenaeus vannamei
spellingShingle Haitao Fan
Xin Zhang
Yanan Yin
Lefei Jiao
Arginine–NO pathway modulated energy metabolism in the hepatopancreas and muscle of female Litopenaeus vannamei
Aquaculture Reports
Arginine
Energy metabolism
Female Litopenaeus vannamei
title Arginine–NO pathway modulated energy metabolism in the hepatopancreas and muscle of female Litopenaeus vannamei
title_full Arginine–NO pathway modulated energy metabolism in the hepatopancreas and muscle of female Litopenaeus vannamei
title_fullStr Arginine–NO pathway modulated energy metabolism in the hepatopancreas and muscle of female Litopenaeus vannamei
title_full_unstemmed Arginine–NO pathway modulated energy metabolism in the hepatopancreas and muscle of female Litopenaeus vannamei
title_short Arginine–NO pathway modulated energy metabolism in the hepatopancreas and muscle of female Litopenaeus vannamei
title_sort arginine no pathway modulated energy metabolism in the hepatopancreas and muscle of female litopenaeus vannamei
topic Arginine
Energy metabolism
Female Litopenaeus vannamei
url http://www.sciencedirect.com/science/article/pii/S2352513424006252
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AT yananyin argininenopathwaymodulatedenergymetabolisminthehepatopancreasandmuscleoffemalelitopenaeusvannamei
AT lefeijiao argininenopathwaymodulatedenergymetabolisminthehepatopancreasandmuscleoffemalelitopenaeusvannamei