The Comparison of the Immunologic Properties of Stem Cells Isolated from Human Exfoliated Deciduous Teeth, Dental Pulp, and Dental Follicles
Aim. To compare the effects of various mesenchymal stem cells, those isolated from human exfoliated deciduous teeth (SHEDs), dental pulp stem cells (DPSCs), and dental follicle stem cells (DFSCs), on human peripheral blood mononuclear cells (PBMCs). Method. Mesenchymal stem cells were isolated from...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2016/4682875 |
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| author | Selin Yildirim Noushin Zibandeh Deniz Genc Elif Merve Ozcan Kamil Goker Tunc Akkoc |
| author_facet | Selin Yildirim Noushin Zibandeh Deniz Genc Elif Merve Ozcan Kamil Goker Tunc Akkoc |
| author_sort | Selin Yildirim |
| collection | DOAJ |
| description | Aim. To compare the effects of various mesenchymal stem cells, those isolated from human exfoliated deciduous teeth (SHEDs), dental pulp stem cells (DPSCs), and dental follicle stem cells (DFSCs), on human peripheral blood mononuclear cells (PBMCs). Method. Mesenchymal stem cells were isolated from three sources in the orofacial region. Characterization and PCR analyses were performed. Lymphocytes were isolated from healthy peripheral venous blood. Lymphocytes were cocultured with stem cells in the presence and absence of IFN-γ and stimulated with anti-CD2, anti-CD3, and anti-CD28 for 3 days. Then, lymphocyte proliferation, the number of CD4+FoxP3+ T regulatory cells, and the levels of Fas/Fas ligand, IL-4, IL-10, and IFN-γ in the culture supernatant were measured. Results. The DFSCs exhibited an enhanced differentiation capacity and an increased number of CD4+FoxP3+ T lymphocytes and suppressed the proliferation and apoptosis of PBMCs compared with SHEDs and DPSCs. The addition of IFN-γ augmented the proliferation of DFSCs. Furthermore, the DFSCs suppressed IL-4 and IFN-γ cytokine levels and enhanced IL-10 levels compared with the other cell sources. Conclusion. These results suggest that IFN-γ stimulates DFSCs by inducing an immunomodulatory effect on the PBMCs of healthy donors while suppressing apoptosis and proliferation and increasing the number of CD4+FoxP3+ cells. |
| format | Article |
| id | doaj-art-ab15941cc3594d4ab201a0a7664be05d |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-ab15941cc3594d4ab201a0a7664be05d2025-02-03T06:01:50ZengWileyStem Cells International1687-966X1687-96782016-01-01201610.1155/2016/46828754682875The Comparison of the Immunologic Properties of Stem Cells Isolated from Human Exfoliated Deciduous Teeth, Dental Pulp, and Dental FolliclesSelin Yildirim0Noushin Zibandeh1Deniz Genc2Elif Merve Ozcan3Kamil Goker4Tunc Akkoc5Division of Pediatric Allergy-Immunology, Faculty of Medicine, Marmara University, 34890 Istanbul, TurkeyDivision of Pediatric Allergy-Immunology, Faculty of Medicine, Marmara University, 34890 Istanbul, TurkeyDivision of Pediatric Allergy-Immunology, Faculty of Medicine, Marmara University, 34890 Istanbul, TurkeyDepartment of Oral and Maxillofacial Surgery, Faculty of Dentistry, Marmara University, 34890 Istanbul, TurkeyDepartment of Oral and Maxillofacial Surgery, Faculty of Dentistry, Marmara University, 34890 Istanbul, TurkeyDivision of Pediatric Allergy-Immunology, Faculty of Medicine, Marmara University, 34890 Istanbul, TurkeyAim. To compare the effects of various mesenchymal stem cells, those isolated from human exfoliated deciduous teeth (SHEDs), dental pulp stem cells (DPSCs), and dental follicle stem cells (DFSCs), on human peripheral blood mononuclear cells (PBMCs). Method. Mesenchymal stem cells were isolated from three sources in the orofacial region. Characterization and PCR analyses were performed. Lymphocytes were isolated from healthy peripheral venous blood. Lymphocytes were cocultured with stem cells in the presence and absence of IFN-γ and stimulated with anti-CD2, anti-CD3, and anti-CD28 for 3 days. Then, lymphocyte proliferation, the number of CD4+FoxP3+ T regulatory cells, and the levels of Fas/Fas ligand, IL-4, IL-10, and IFN-γ in the culture supernatant were measured. Results. The DFSCs exhibited an enhanced differentiation capacity and an increased number of CD4+FoxP3+ T lymphocytes and suppressed the proliferation and apoptosis of PBMCs compared with SHEDs and DPSCs. The addition of IFN-γ augmented the proliferation of DFSCs. Furthermore, the DFSCs suppressed IL-4 and IFN-γ cytokine levels and enhanced IL-10 levels compared with the other cell sources. Conclusion. These results suggest that IFN-γ stimulates DFSCs by inducing an immunomodulatory effect on the PBMCs of healthy donors while suppressing apoptosis and proliferation and increasing the number of CD4+FoxP3+ cells.http://dx.doi.org/10.1155/2016/4682875 |
| spellingShingle | Selin Yildirim Noushin Zibandeh Deniz Genc Elif Merve Ozcan Kamil Goker Tunc Akkoc The Comparison of the Immunologic Properties of Stem Cells Isolated from Human Exfoliated Deciduous Teeth, Dental Pulp, and Dental Follicles Stem Cells International |
| title | The Comparison of the Immunologic Properties of Stem Cells Isolated from Human Exfoliated Deciduous Teeth, Dental Pulp, and Dental Follicles |
| title_full | The Comparison of the Immunologic Properties of Stem Cells Isolated from Human Exfoliated Deciduous Teeth, Dental Pulp, and Dental Follicles |
| title_fullStr | The Comparison of the Immunologic Properties of Stem Cells Isolated from Human Exfoliated Deciduous Teeth, Dental Pulp, and Dental Follicles |
| title_full_unstemmed | The Comparison of the Immunologic Properties of Stem Cells Isolated from Human Exfoliated Deciduous Teeth, Dental Pulp, and Dental Follicles |
| title_short | The Comparison of the Immunologic Properties of Stem Cells Isolated from Human Exfoliated Deciduous Teeth, Dental Pulp, and Dental Follicles |
| title_sort | comparison of the immunologic properties of stem cells isolated from human exfoliated deciduous teeth dental pulp and dental follicles |
| url | http://dx.doi.org/10.1155/2016/4682875 |
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