Induction of Expression of CD271 and CD34 in Mesenchymal Stromal Cells Cultured as Spheroids
Cultured mesenchymal stromal cells (MSCs) are cells that can be used for tissue engineering or cell therapies owing to their multipotency and ability to secrete immunomodulatory and trophic molecules. Several studies suggest that MSCs can become pericytes when cocultured with endothelial cells (ECs)...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2018/7357213 |
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| author | Bruno Corrêa Bellagamba Patrícia Bencke Grudzinski Pedro Bins Ely Paulo de Jesus Hartmann Nader Nance Beyer Nardi Lindolfo da Silva Meirelles |
| author_facet | Bruno Corrêa Bellagamba Patrícia Bencke Grudzinski Pedro Bins Ely Paulo de Jesus Hartmann Nader Nance Beyer Nardi Lindolfo da Silva Meirelles |
| author_sort | Bruno Corrêa Bellagamba |
| collection | DOAJ |
| description | Cultured mesenchymal stromal cells (MSCs) are cells that can be used for tissue engineering or cell therapies owing to their multipotency and ability to secrete immunomodulatory and trophic molecules. Several studies suggest that MSCs can become pericytes when cocultured with endothelial cells (ECs) but failed to use pericyte markers not already expressed by MSCs. We hypothesized ECs could instruct MSCs to express the molecules CD271 or CD34, which are expressed by pericytes in situ but not by MSCs. CD271 is a marker of especial interest because it is associated with multipotency, a characteristic that wanes in MSCs as they are culture expanded. Consequently, surface expression of CD271 and CD34 was detected in roughly half of the MSCs cocultured with ECs as spheroids in the presence of insulin-like growth factor 1 (IGF-1). Conversely, expression of CD271 and CD34 was detected in a similar proportion of MSCs cultured under these conditions without ECs, and expression of these markers was low or absent when no IGF-1 was added. These findings indicate that specific culture conditions including IGF-1 can endow cultured MSCs with expression of CD271 and CD34, which may enhance the multipotency of these cells when they are used for therapeutic purposes. |
| format | Article |
| id | doaj-art-ab0dc4fbf14e4a028eade010031d348f |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-ab0dc4fbf14e4a028eade010031d348f2025-02-03T01:11:00ZengWileyStem Cells International1687-966X1687-96782018-01-01201810.1155/2018/73572137357213Induction of Expression of CD271 and CD34 in Mesenchymal Stromal Cells Cultured as SpheroidsBruno Corrêa Bellagamba0Patrícia Bencke Grudzinski1Pedro Bins Ely2Paulo de Jesus Hartmann Nader3Nance Beyer Nardi4Lindolfo da Silva Meirelles5Laboratory for Stem Cells and Tissue Engineering, PPGBioSaúde, Universidade Luterana do Brasil, Canoas, RS, BrazilLaboratory for Stem Cells and Tissue Engineering, PPGBioSaúde, Universidade Luterana do Brasil, Canoas, RS, BrazilPlastic Surgery Service, Irmandade Santa Casa de Misericórdia de Porto Alegre, Porto Alegre, RS, BrazilPediatrics and Neonatology Service, Hospital Universitário ULBRA Mãe de Deus, Canoas, RS, BrazilLaboratory for Stem Cells and Tissue Engineering, PPGBioSaúde, Universidade Luterana do Brasil, Canoas, RS, BrazilLaboratory for Stem Cells and Tissue Engineering, PPGBioSaúde, Universidade Luterana do Brasil, Canoas, RS, BrazilCultured mesenchymal stromal cells (MSCs) are cells that can be used for tissue engineering or cell therapies owing to their multipotency and ability to secrete immunomodulatory and trophic molecules. Several studies suggest that MSCs can become pericytes when cocultured with endothelial cells (ECs) but failed to use pericyte markers not already expressed by MSCs. We hypothesized ECs could instruct MSCs to express the molecules CD271 or CD34, which are expressed by pericytes in situ but not by MSCs. CD271 is a marker of especial interest because it is associated with multipotency, a characteristic that wanes in MSCs as they are culture expanded. Consequently, surface expression of CD271 and CD34 was detected in roughly half of the MSCs cocultured with ECs as spheroids in the presence of insulin-like growth factor 1 (IGF-1). Conversely, expression of CD271 and CD34 was detected in a similar proportion of MSCs cultured under these conditions without ECs, and expression of these markers was low or absent when no IGF-1 was added. These findings indicate that specific culture conditions including IGF-1 can endow cultured MSCs with expression of CD271 and CD34, which may enhance the multipotency of these cells when they are used for therapeutic purposes.http://dx.doi.org/10.1155/2018/7357213 |
| spellingShingle | Bruno Corrêa Bellagamba Patrícia Bencke Grudzinski Pedro Bins Ely Paulo de Jesus Hartmann Nader Nance Beyer Nardi Lindolfo da Silva Meirelles Induction of Expression of CD271 and CD34 in Mesenchymal Stromal Cells Cultured as Spheroids Stem Cells International |
| title | Induction of Expression of CD271 and CD34 in Mesenchymal Stromal Cells Cultured as Spheroids |
| title_full | Induction of Expression of CD271 and CD34 in Mesenchymal Stromal Cells Cultured as Spheroids |
| title_fullStr | Induction of Expression of CD271 and CD34 in Mesenchymal Stromal Cells Cultured as Spheroids |
| title_full_unstemmed | Induction of Expression of CD271 and CD34 in Mesenchymal Stromal Cells Cultured as Spheroids |
| title_short | Induction of Expression of CD271 and CD34 in Mesenchymal Stromal Cells Cultured as Spheroids |
| title_sort | induction of expression of cd271 and cd34 in mesenchymal stromal cells cultured as spheroids |
| url | http://dx.doi.org/10.1155/2018/7357213 |
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