A Strategy for the Production of Single-Cell Proteins by the Efficient and Continuous Fermentation of Hydroxide Bacteria Under Gas Fermentation

A Strategy for the Production of Single-Cell Proteins by the Efficient and Continuous Fermentation of Hydroxide Bacteria Under Gas Fermentation

<i>Cupriavidus necator</i> can produce single-cell proteins (SCPs) using electrons produced by hydrogen as energy, oxygen as electron acceptors, and CO<sub>2</sub> as carbon sources. Gas fermentation is a process of microbial fermentation that uses gas substrates (such as hyd...

Full description

Saved in:
Bibliographic Details
Main Authors: Shuai Fu, Longyu Gou, Ke Long, Lanchai Chen, Dingrong Cai, Yue Lu
Format: Article
Language:English
Published: MDPI AG 2025-01-01
Series:Applied Sciences
Subjects:
<i>Cupriavidus necator</i>
SCPs
CO<sub>2</sub>
self-developed bioreactor
high-efficiency continuous fermentation
Online Access:https://www.mdpi.com/2076-3417/15/2/540
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:<i>Cupriavidus necator</i> can produce single-cell proteins (SCPs) using electrons produced by hydrogen as energy, oxygen as electron acceptors, and CO<sub>2</sub> as carbon sources. Gas fermentation is a process of microbial fermentation that uses gas substrates (such as hydrogen, carbon dioxide, etc.) which faces several challenges, mainly including the low solubility of gas substrates, the danger of hydrogen and oxygen mixing, and the optimization of fermentation conditions. To overcome these challenges, this article explores a variety of strategies—including the design of a self-developed bioreactor—to reduce the risk of static electricity. Without the addition of filler material, the results showed that the maximum cell dry weight (CDW) of 30% secondary seed inoculation was 20.41% higher than that of 10% secondary seed inoculum, and 5.99% higher than that of 20% secondary seed inoculum. Combined with the filler material and with the use of high-efficiency continuous fermentation technology, the average yield of continuous fermentation was 23.31 g/day, while the average yield of batch fermentation was 14.33 g/day. The daily yield of continuous fermentation is 1.63 times that of batch fermentation. These efforts are aimed at improving the efficiency and safety of gas fermentation.
ISSN:2076-3417

Similar Items