Predatory bacteria can intensify lung-injury in a multidrug-resistant Acinetobacter baumannii pneumonia model in rat
IntroductionRespiratory tract infection caused by antibiotic-resistant bacteria are one of the most important causes of death worldwide. Therefore, in this study, we investigated the possibility of using predatory bacteria to improve the Acinetobacter baumannii pneumonia model in rat.MethodsMultidru...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1512119/full |
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| author | Zeinab Mohsenipour Farzaneh Kianian Behnaz Jahanbin Hamid Reza Abtahi Tooba Ghazanfari Maryam Edalatifard Saeid Amanpour Mikael Skurnik Parya Arazi Mohammad Mehdi Feizabadi Mohammad Mehdi Feizabadi |
| author_facet | Zeinab Mohsenipour Farzaneh Kianian Behnaz Jahanbin Hamid Reza Abtahi Tooba Ghazanfari Maryam Edalatifard Saeid Amanpour Mikael Skurnik Parya Arazi Mohammad Mehdi Feizabadi Mohammad Mehdi Feizabadi |
| author_sort | Zeinab Mohsenipour |
| collection | DOAJ |
| description | IntroductionRespiratory tract infection caused by antibiotic-resistant bacteria are one of the most important causes of death worldwide. Therefore, in this study, we investigated the possibility of using predatory bacteria to improve the Acinetobacter baumannii pneumonia model in rat.MethodsMultidrug-resistant (MDR) A. baumannii clinical strain was used to induce pneumonia. In addition to the sham and predator control group, three treatment groups (n = 5) were studied with colistin, Bdellovibrio bacteriovorus HD100, and combination of predator and antibiotics. Also, the colistin MIC value for B. bacteriovorus HD100 (8 μg/mL) was determined for the first time to our knowledge. Removal of excess endotoxin from the predator suspension was performed with the help of organic solvents before inoculation of rats.ResultsThe most successful treatment was observed in the group treated with colistin followed by combined treatment. In the predator treatment group, the systemic spread of A. baumannii was lower than other treatment groups. However, treatment with predatory bacteria not only failed to reduce the pathogen load in the lungs to the same extent as the antibiotic treatment group, but also induced acute pulmonary and systemic inflammatory responses. Therefore, the rats showed the highest septic score (21.4 at 48 h) and did not survive more than 48 h.DiscussionThis is the first report of systemic complications of using B. bacteriovorus HD100 for infection control. According to our results, the effects of predatory bacteria in the in vivo environment are complex and many questions need to be answered before it can be introduced as a live antibiotic. |
| format | Article |
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| institution | Kabale University |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj-art-aaff0979f3dc4517812c9c75d8403a622025-01-23T06:56:12ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-01-011610.3389/fmicb.2025.15121191512119Predatory bacteria can intensify lung-injury in a multidrug-resistant Acinetobacter baumannii pneumonia model in ratZeinab Mohsenipour0Farzaneh Kianian1Behnaz Jahanbin2Hamid Reza Abtahi3Tooba Ghazanfari4Maryam Edalatifard5Saeid Amanpour6Mikael Skurnik7Parya Arazi8Mohammad Mehdi Feizabadi9Mohammad Mehdi Feizabadi10Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, IranDepartment of Physiology, School of Medicine, Tehran University of Medical Sciences, Tehran, IranDepartment of Pathology, Cancer Institute, Imam Khomeini Hospital Complex, Tehran University of Medical Sciences, Tehran, IranDepartment of Pulmonary and Critical Care, Thoracic Research Center, Imam Khomeini Hospital Complex, Tehran University of Medical Sciences, Tehran, IranImmunoregulation Research Center, Shahed University, Tehran, IranDepartment of Pulmonary and Critical Care, Thoracic Research Center, Imam Khomeini Hospital Complex, Tehran University of Medical Sciences, Tehran, IranCancer Biology Research Center, Tehran University of Medical Sciences, Tehran, IranDepartment of Bacteriology and Immunology, Faculty of Medicine, University of Helsinki, Helsinki, FinlandDepartment of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, IranDepartment of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, IranDepartment of Pulmonary and Critical Care, Thoracic Research Center, Imam Khomeini Hospital Complex, Tehran University of Medical Sciences, Tehran, IranIntroductionRespiratory tract infection caused by antibiotic-resistant bacteria are one of the most important causes of death worldwide. Therefore, in this study, we investigated the possibility of using predatory bacteria to improve the Acinetobacter baumannii pneumonia model in rat.MethodsMultidrug-resistant (MDR) A. baumannii clinical strain was used to induce pneumonia. In addition to the sham and predator control group, three treatment groups (n = 5) were studied with colistin, Bdellovibrio bacteriovorus HD100, and combination of predator and antibiotics. Also, the colistin MIC value for B. bacteriovorus HD100 (8 μg/mL) was determined for the first time to our knowledge. Removal of excess endotoxin from the predator suspension was performed with the help of organic solvents before inoculation of rats.ResultsThe most successful treatment was observed in the group treated with colistin followed by combined treatment. In the predator treatment group, the systemic spread of A. baumannii was lower than other treatment groups. However, treatment with predatory bacteria not only failed to reduce the pathogen load in the lungs to the same extent as the antibiotic treatment group, but also induced acute pulmonary and systemic inflammatory responses. Therefore, the rats showed the highest septic score (21.4 at 48 h) and did not survive more than 48 h.DiscussionThis is the first report of systemic complications of using B. bacteriovorus HD100 for infection control. According to our results, the effects of predatory bacteria in the in vivo environment are complex and many questions need to be answered before it can be introduced as a live antibiotic.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1512119/fullAcinetobacter baumanniiantibiotic resistantBdellovibrio bacteriovoruspredatory bacteriarespiratory tract infection |
| spellingShingle | Zeinab Mohsenipour Farzaneh Kianian Behnaz Jahanbin Hamid Reza Abtahi Tooba Ghazanfari Maryam Edalatifard Saeid Amanpour Mikael Skurnik Parya Arazi Mohammad Mehdi Feizabadi Mohammad Mehdi Feizabadi Predatory bacteria can intensify lung-injury in a multidrug-resistant Acinetobacter baumannii pneumonia model in rat Frontiers in Microbiology Acinetobacter baumannii antibiotic resistant Bdellovibrio bacteriovorus predatory bacteria respiratory tract infection |
| title | Predatory bacteria can intensify lung-injury in a multidrug-resistant Acinetobacter baumannii pneumonia model in rat |
| title_full | Predatory bacteria can intensify lung-injury in a multidrug-resistant Acinetobacter baumannii pneumonia model in rat |
| title_fullStr | Predatory bacteria can intensify lung-injury in a multidrug-resistant Acinetobacter baumannii pneumonia model in rat |
| title_full_unstemmed | Predatory bacteria can intensify lung-injury in a multidrug-resistant Acinetobacter baumannii pneumonia model in rat |
| title_short | Predatory bacteria can intensify lung-injury in a multidrug-resistant Acinetobacter baumannii pneumonia model in rat |
| title_sort | predatory bacteria can intensify lung injury in a multidrug resistant acinetobacter baumannii pneumonia model in rat |
| topic | Acinetobacter baumannii antibiotic resistant Bdellovibrio bacteriovorus predatory bacteria respiratory tract infection |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1512119/full |
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