Overexpression of MMP13 Is Associated with Clinical Outcomes and Poor Prognosis in Oral Squamous Cell Carcinoma
Matrix metalloproteinase 13 (MMP13) plays a central role in the MMP activation cascade that enables degradation of the extracellular matrix and basement membranes, and it is identified as a potential driver in oral carcinogenesis. Therefore, this study aims to determine the copy number, mRNA, and pr...
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| Language: | English |
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Wiley
2014-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/897523 |
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| author | Vui King Vincent-Chong Iman Salahshourifar Lee Peng Karen-Ng Ming Yhong Siow Thomas George Kallarakkal Anand Ramanathan Yi-Hsin Yang Goot Heah Khor Zainal Ariff Abdul Rahman Siti Mazlipah Ismail Narayanan Prepageran Wan Mahadzir Wan Mustafa Mannil Thomas Abraham Keng Kiong Tay Sok Ching Cheong Rosnah Binti Zain |
| author_facet | Vui King Vincent-Chong Iman Salahshourifar Lee Peng Karen-Ng Ming Yhong Siow Thomas George Kallarakkal Anand Ramanathan Yi-Hsin Yang Goot Heah Khor Zainal Ariff Abdul Rahman Siti Mazlipah Ismail Narayanan Prepageran Wan Mahadzir Wan Mustafa Mannil Thomas Abraham Keng Kiong Tay Sok Ching Cheong Rosnah Binti Zain |
| author_sort | Vui King Vincent-Chong |
| collection | DOAJ |
| description | Matrix metalloproteinase 13 (MMP13) plays a central role in the MMP activation cascade that enables degradation of the extracellular matrix and basement membranes, and it is identified as a potential driver in oral carcinogenesis. Therefore, this study aims to determine the copy number, mRNA, and protein expression of MMP13 in oral squamous cell carcinoma (OSCC) and to associate these expressions with clinicopathological parameters. Copy number, mRNA, and protein expression analysis of MMP13 were determined using real-time quantitative PCR and immunohistochemistry methods in OSCC samples. The correlations between MMP13 expressions and clinicopathological parameters were evaluated, and the significance of MMP13 as a prognostic factor was determined. Despite discrepancies between gene amplification and mRNA and protein overexpression rates, OSCC cases showed high amplification of MMP13 and overexpression of MMP13 at both mRNA and protein levels. High level of MMP13 protein expression showed a significant correlation with lymph node metastasis (P=0.011) and tumor staging (P=0.002). Multivariate Cox regression model analysis revealed that high level of mRNA and protein expression of MMP13 were significantly associated with poor prognosis (P<0.050). Taken together, these observations indicate that the MMP13 protein overexpression could be considered as a prognostic marker of OSCC. |
| format | Article |
| id | doaj-art-aad56cf16fbd427192cf89d8221db4cd |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-aad56cf16fbd427192cf89d8221db4cd2025-02-03T01:21:22ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/897523897523Overexpression of MMP13 Is Associated with Clinical Outcomes and Poor Prognosis in Oral Squamous Cell CarcinomaVui King Vincent-Chong0Iman Salahshourifar1Lee Peng Karen-Ng2Ming Yhong Siow3Thomas George Kallarakkal4Anand Ramanathan5Yi-Hsin Yang6Goot Heah Khor7Zainal Ariff Abdul Rahman8Siti Mazlipah Ismail9Narayanan Prepageran10Wan Mahadzir Wan Mustafa11Mannil Thomas Abraham12Keng Kiong Tay13Sok Ching Cheong14Rosnah Binti Zain15Oral Cancer Research and Coordinating Centre, Faculty of Dentistry, University of Malaya, 50603 Kuala Lumpur, MalaysiaOral Cancer Research and Coordinating Centre, Faculty of Dentistry, University of Malaya, 50603 Kuala Lumpur, MalaysiaOral Cancer Research and Coordinating Centre, Faculty of Dentistry, University of Malaya, 50603 Kuala Lumpur, MalaysiaOral Cancer Research and Coordinating Centre, Faculty of Dentistry, University of Malaya, 50603 Kuala Lumpur, MalaysiaOral Cancer Research and Coordinating Centre, Faculty of Dentistry, University of Malaya, 50603 Kuala Lumpur, MalaysiaOral Cancer Research and Coordinating Centre, Faculty of Dentistry, University of Malaya, 50603 Kuala Lumpur, MalaysiaSchool of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, TaiwanCentre of Preclinical Science Studies, Faculty of Dentistry, Universiti Teknologi MARA, 40450 Shah Alam, Selangor Darul Ehsan, MalaysiaOral Cancer Research and Coordinating Centre, Faculty of Dentistry, University of Malaya, 50603 Kuala Lumpur, MalaysiaOral Cancer Research and Coordinating Centre, Faculty of Dentistry, University of Malaya, 50603 Kuala Lumpur, MalaysiaOral Cancer Research and Coordinating Centre, Faculty of Dentistry, University of Malaya, 50603 Kuala Lumpur, MalaysiaOral Cancer Research and Coordinating Centre, Faculty of Dentistry, University of Malaya, 50603 Kuala Lumpur, MalaysiaOral Cancer Research and Coordinating Centre, Faculty of Dentistry, University of Malaya, 50603 Kuala Lumpur, MalaysiaOral Cancer Research and Coordinating Centre, Faculty of Dentistry, University of Malaya, 50603 Kuala Lumpur, MalaysiaDepartment of Oro-Maxillofacial Surgical and Medical Sciences, Faculty of Dentistry, University of Malaya, 50603 Kuala Lumpur, MalaysiaOral Cancer Research and Coordinating Centre, Faculty of Dentistry, University of Malaya, 50603 Kuala Lumpur, MalaysiaMatrix metalloproteinase 13 (MMP13) plays a central role in the MMP activation cascade that enables degradation of the extracellular matrix and basement membranes, and it is identified as a potential driver in oral carcinogenesis. Therefore, this study aims to determine the copy number, mRNA, and protein expression of MMP13 in oral squamous cell carcinoma (OSCC) and to associate these expressions with clinicopathological parameters. Copy number, mRNA, and protein expression analysis of MMP13 were determined using real-time quantitative PCR and immunohistochemistry methods in OSCC samples. The correlations between MMP13 expressions and clinicopathological parameters were evaluated, and the significance of MMP13 as a prognostic factor was determined. Despite discrepancies between gene amplification and mRNA and protein overexpression rates, OSCC cases showed high amplification of MMP13 and overexpression of MMP13 at both mRNA and protein levels. High level of MMP13 protein expression showed a significant correlation with lymph node metastasis (P=0.011) and tumor staging (P=0.002). Multivariate Cox regression model analysis revealed that high level of mRNA and protein expression of MMP13 were significantly associated with poor prognosis (P<0.050). Taken together, these observations indicate that the MMP13 protein overexpression could be considered as a prognostic marker of OSCC.http://dx.doi.org/10.1155/2014/897523 |
| spellingShingle | Vui King Vincent-Chong Iman Salahshourifar Lee Peng Karen-Ng Ming Yhong Siow Thomas George Kallarakkal Anand Ramanathan Yi-Hsin Yang Goot Heah Khor Zainal Ariff Abdul Rahman Siti Mazlipah Ismail Narayanan Prepageran Wan Mahadzir Wan Mustafa Mannil Thomas Abraham Keng Kiong Tay Sok Ching Cheong Rosnah Binti Zain Overexpression of MMP13 Is Associated with Clinical Outcomes and Poor Prognosis in Oral Squamous Cell Carcinoma The Scientific World Journal |
| title | Overexpression of MMP13 Is Associated with Clinical Outcomes and Poor Prognosis in Oral Squamous Cell Carcinoma |
| title_full | Overexpression of MMP13 Is Associated with Clinical Outcomes and Poor Prognosis in Oral Squamous Cell Carcinoma |
| title_fullStr | Overexpression of MMP13 Is Associated with Clinical Outcomes and Poor Prognosis in Oral Squamous Cell Carcinoma |
| title_full_unstemmed | Overexpression of MMP13 Is Associated with Clinical Outcomes and Poor Prognosis in Oral Squamous Cell Carcinoma |
| title_short | Overexpression of MMP13 Is Associated with Clinical Outcomes and Poor Prognosis in Oral Squamous Cell Carcinoma |
| title_sort | overexpression of mmp13 is associated with clinical outcomes and poor prognosis in oral squamous cell carcinoma |
| url | http://dx.doi.org/10.1155/2014/897523 |
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