Retrotransposon-Based Genetic Diversity of <i>Rhodiola rosea</i> L. (Crassulaceae) from Kazakhstan Altai
The analysis of genetic diversity in natural populations of valuable medicinal plant species experiencing overexploitation is a key aspect of their natural conservation strategy. Mobile genetic elements and other interspersed repeats, which are major components of eukaryotic genomes, serve as effect...
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2025-01-01
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| author | Oxana Khapilina Ainur Turzhanova Moldir Zhumagul Damelya Tagimanova Olesya Raiser Serik Kubentayev Vladislav Shevtsov Maria Hohn |
| author_facet | Oxana Khapilina Ainur Turzhanova Moldir Zhumagul Damelya Tagimanova Olesya Raiser Serik Kubentayev Vladislav Shevtsov Maria Hohn |
| author_sort | Oxana Khapilina |
| collection | DOAJ |
| description | The analysis of genetic diversity in natural populations of valuable medicinal plant species experiencing overexploitation is a key aspect of their natural conservation strategy. Mobile genetic elements and other interspersed repeats, which are major components of eukaryotic genomes, serve as effective tools for studying plant biodiversity and variability. The genetic diversity of four valuable medicinal plant <i>Rhodiola rosea</i> L. populations was investigated using the inter-repeat amplified PCR method with inter-priming binding sites (iPBSs) for genome profiling. At the interpopulation level, unique amplicons characteristic of specific <i>R. rosea</i> populations were identified. Molecular variance analysis revealed that the biodiversity of <i>R. rosea</i> populations in the Kazakh Altai region is 56% attributed to interpopulation differences and 44% to intrapopulation differences. It was shown that populations located in favorable environmental conditions have greater genetic diversity compared to those in extreme habitats. This study identified a high degree of polymorphism among <i>R. rosea</i> populations using the inter-repeat amplified PCR method. The genetic diversity of the populations ranged from 0.105 to 0.156, with an average heterozygosity of 0.134. The findings provide new insights into the population structure of <i>R. rosea</i> in the Kazakh Altai, enabling the identification of different genotypes, which will significantly complement traditional methods for conserving this valuable medicinal plant. |
| format | Article |
| id | doaj-art-aaba1d091eed4d54a980cedcc8cb7fe9 |
| institution | Kabale University |
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| language | English |
| publishDate | 2025-01-01 |
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| spelling | doaj-art-aaba1d091eed4d54a980cedcc8cb7fe92025-01-24T13:29:28ZengMDPI AGDiversity1424-28182025-01-011714510.3390/d17010045Retrotransposon-Based Genetic Diversity of <i>Rhodiola rosea</i> L. (Crassulaceae) from Kazakhstan AltaiOxana Khapilina0Ainur Turzhanova1Moldir Zhumagul2Damelya Tagimanova3Olesya Raiser4Serik Kubentayev5Vladislav Shevtsov6Maria Hohn7National Center for Biotechnology, Qorghalzhyn Hwy 13, Astana 010000, KazakhstanNational Center for Biotechnology, Qorghalzhyn Hwy 13, Astana 010000, KazakhstanDepartment of Graduate School of Natural Sciences, Astana International University, Kabanbai Batyr 8, Astana 010000, KazakhstanNational Center for Biotechnology, Qorghalzhyn Hwy 13, Astana 010000, KazakhstanNational Center for Biotechnology, Qorghalzhyn Hwy 13, Astana 010000, KazakhstanAstana Botanical Garden, Orynbor 16, Astana 010000, KazakhstanNational Center for Biotechnology, Qorghalzhyn Hwy 13, Astana 010000, KazakhstanDepartment of Botany, Institute of Agronomy, Hungarian University of Agriculture and Life Sciences, H-1118 Budapest, HungaryThe analysis of genetic diversity in natural populations of valuable medicinal plant species experiencing overexploitation is a key aspect of their natural conservation strategy. Mobile genetic elements and other interspersed repeats, which are major components of eukaryotic genomes, serve as effective tools for studying plant biodiversity and variability. The genetic diversity of four valuable medicinal plant <i>Rhodiola rosea</i> L. populations was investigated using the inter-repeat amplified PCR method with inter-priming binding sites (iPBSs) for genome profiling. At the interpopulation level, unique amplicons characteristic of specific <i>R. rosea</i> populations were identified. Molecular variance analysis revealed that the biodiversity of <i>R. rosea</i> populations in the Kazakh Altai region is 56% attributed to interpopulation differences and 44% to intrapopulation differences. It was shown that populations located in favorable environmental conditions have greater genetic diversity compared to those in extreme habitats. This study identified a high degree of polymorphism among <i>R. rosea</i> populations using the inter-repeat amplified PCR method. The genetic diversity of the populations ranged from 0.105 to 0.156, with an average heterozygosity of 0.134. The findings provide new insights into the population structure of <i>R. rosea</i> in the Kazakh Altai, enabling the identification of different genotypes, which will significantly complement traditional methods for conserving this valuable medicinal plant.https://www.mdpi.com/1424-2818/17/1/45<i>Rhodiola rosea</i>genetic diversityiPBS profilingmolecular markermedicinal plant |
| spellingShingle | Oxana Khapilina Ainur Turzhanova Moldir Zhumagul Damelya Tagimanova Olesya Raiser Serik Kubentayev Vladislav Shevtsov Maria Hohn Retrotransposon-Based Genetic Diversity of <i>Rhodiola rosea</i> L. (Crassulaceae) from Kazakhstan Altai Diversity <i>Rhodiola rosea</i> genetic diversity iPBS profiling molecular marker medicinal plant |
| title | Retrotransposon-Based Genetic Diversity of <i>Rhodiola rosea</i> L. (Crassulaceae) from Kazakhstan Altai |
| title_full | Retrotransposon-Based Genetic Diversity of <i>Rhodiola rosea</i> L. (Crassulaceae) from Kazakhstan Altai |
| title_fullStr | Retrotransposon-Based Genetic Diversity of <i>Rhodiola rosea</i> L. (Crassulaceae) from Kazakhstan Altai |
| title_full_unstemmed | Retrotransposon-Based Genetic Diversity of <i>Rhodiola rosea</i> L. (Crassulaceae) from Kazakhstan Altai |
| title_short | Retrotransposon-Based Genetic Diversity of <i>Rhodiola rosea</i> L. (Crassulaceae) from Kazakhstan Altai |
| title_sort | retrotransposon based genetic diversity of i rhodiola rosea i l crassulaceae from kazakhstan altai |
| topic | <i>Rhodiola rosea</i> genetic diversity iPBS profiling molecular marker medicinal plant |
| url | https://www.mdpi.com/1424-2818/17/1/45 |
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