Investigation of some important pathogens in bulls
In this study, presence of some important pathogens such as Bovine Herpesvirus 1 (BHV-1), Bovine Viral Diarrhea Virus (BVDV), Campylobacter, Brucella and Mycoplasma were investigated by viral and bacteriological isolation, PCR/RT-PCR and serological methods in bulls. Seventy three semen, 94 preputia...
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| Format: | Article |
| Language: | English |
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Selcuk University Press
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| Series: | Eurasian Journal of Veterinary Sciences |
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| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=157 |
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| author | Leyla Güler Sibel Yavru Mehmet Ekik Ümran Ok Atilla Şimşek Kadir Gündüz Yasin Gülcü |
| author_facet | Leyla Güler Sibel Yavru Mehmet Ekik Ümran Ok Atilla Şimşek Kadir Gündüz Yasin Gülcü |
| author_sort | Leyla Güler |
| collection | DOAJ |
| description | In this study, presence of some important pathogens such as Bovine Herpesvirus 1 (BHV-1), Bovine Viral Diarrhea Virus (BVDV), Campylobacter, Brucella and Mycoplasma were investigated by viral and bacteriological isolation, PCR/RT-PCR and serological methods in bulls. Seventy three semen, 94 preputial washings and 101 serum samples from bulls above 1 year old brought in slaughterhouse and, 210 extended semen samples collected in different dates from 19 bulls in an artificial insemination center and their sera were examined. BHV-1 and BVDV were not determined by PCR/RT-PCR and virus isolation in semen samples. Brucella and Campylobacter were not isolated in any of semen and preputial washing samples. Mycoplasma spp. was isolated in 24 (25.5%) of 94 preputial washing samples. In 120 bull sera (19 sera from an artificial insemination center, 101 from slaughterhouse) tested by ELISA, 42 (35 %) were positive for BHV-1 and 46 (39.6 %) for BVDV antibodies. Of the 19 bulls in an artificial insemination center all were seropositive for BHV-1 and 4 were seropositive for BVDV. All serum samples were negative for Brucella antibodies. None of the bulls examined in this study had any clinical symptoms. |
| format | Article |
| id | doaj-art-aab95fa4050846c59534f799411acd32 |
| institution | Kabale University |
| issn | 1309-6958 2146-1953 |
| language | English |
| publisher | Selcuk University Press |
| record_format | Article |
| series | Eurasian Journal of Veterinary Sciences |
| spelling | doaj-art-aab95fa4050846c59534f799411acd322025-02-03T11:25:44ZengSelcuk University PressEurasian Journal of Veterinary Sciences1309-69582146-19532047178157Investigation of some important pathogens in bullsLeyla Güler0Sibel Yavru1Mehmet Ekik2Ümran Ok3Atilla Şimşek4Kadir Gündüz5Yasin Gülcü6Konya Veteriner Kontrol ve Araştırma Enstitüsü, KONYASelçuk Üniversitesi Veteriner Fakültesi, Viroloji Anabilim Dalı, KONYAKonya Veteriner Kontrol ve Araştırma Enstitüsü, KONYAKonya Veteriner Kontrol ve Araştırma Enstitüsü, KONYASelçuk Üniversitesi Veteriner Fakültesi, Viroloji Anabilim Dalı, KONYAKonya Veteriner Kontrol ve Araştırma Enstitüsü, KONYAKonya Veteriner Kontrol ve Araştırma Enstitüsü, KONYAIn this study, presence of some important pathogens such as Bovine Herpesvirus 1 (BHV-1), Bovine Viral Diarrhea Virus (BVDV), Campylobacter, Brucella and Mycoplasma were investigated by viral and bacteriological isolation, PCR/RT-PCR and serological methods in bulls. Seventy three semen, 94 preputial washings and 101 serum samples from bulls above 1 year old brought in slaughterhouse and, 210 extended semen samples collected in different dates from 19 bulls in an artificial insemination center and their sera were examined. BHV-1 and BVDV were not determined by PCR/RT-PCR and virus isolation in semen samples. Brucella and Campylobacter were not isolated in any of semen and preputial washing samples. Mycoplasma spp. was isolated in 24 (25.5%) of 94 preputial washing samples. In 120 bull sera (19 sera from an artificial insemination center, 101 from slaughterhouse) tested by ELISA, 42 (35 %) were positive for BHV-1 and 46 (39.6 %) for BVDV antibodies. Of the 19 bulls in an artificial insemination center all were seropositive for BHV-1 and 4 were seropositive for BVDV. All serum samples were negative for Brucella antibodies. None of the bulls examined in this study had any clinical symptoms.http://eurasianjvetsci.org/pdf.php3?id=157bullsemenbhv-1bvdv. pcrrt-pcrcampylobacterbrucellamycoplasma |
| spellingShingle | Leyla Güler Sibel Yavru Mehmet Ekik Ümran Ok Atilla Şimşek Kadir Gündüz Yasin Gülcü Investigation of some important pathogens in bulls Eurasian Journal of Veterinary Sciences bull semen bhv-1 bvdv. pcr rt-pcr campylobacter brucella mycoplasma |
| title | Investigation of some important pathogens in bulls |
| title_full | Investigation of some important pathogens in bulls |
| title_fullStr | Investigation of some important pathogens in bulls |
| title_full_unstemmed | Investigation of some important pathogens in bulls |
| title_short | Investigation of some important pathogens in bulls |
| title_sort | investigation of some important pathogens in bulls |
| topic | bull semen bhv-1 bvdv. pcr rt-pcr campylobacter brucella mycoplasma |
| url | http://eurasianjvetsci.org/pdf.php3?id=157 |
| work_keys_str_mv | AT leylaguler investigationofsomeimportantpathogensinbulls AT sibelyavru investigationofsomeimportantpathogensinbulls AT mehmetekik investigationofsomeimportantpathogensinbulls AT umranok investigationofsomeimportantpathogensinbulls AT atillasimsek investigationofsomeimportantpathogensinbulls AT kadirgunduz investigationofsomeimportantpathogensinbulls AT yasingulcu investigationofsomeimportantpathogensinbulls |