Fecal carriage of carbapenemase and AmpC-β-lactamase producers among extended spectrum β-Lactamase-producing E. coli and Klebsiella spp. isolates in patients attending hospitals
Abstract Background Extended-spectrum β-lactamase-producing Enterobacterales (ESBL-PE), particularly Escherichia coli and Klebsiella pneumoniae, have been consistently associated with treatment failure, high mortality and morbidity. The emergence of carbapenem resistance among ESBL-PE strains exacer...
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2025-01-01
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| Online Access: | https://doi.org/10.1186/s12879-025-10506-4 |
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| author | Zakaria Garba Isidore J. O. Bonkoungou Namwin Siourimè Somda Magloire H. Natama Georges Somé Lassana Sangaré Nicolas Barro Halidou Tinto |
| author_facet | Zakaria Garba Isidore J. O. Bonkoungou Namwin Siourimè Somda Magloire H. Natama Georges Somé Lassana Sangaré Nicolas Barro Halidou Tinto |
| author_sort | Zakaria Garba |
| collection | DOAJ |
| description | Abstract Background Extended-spectrum β-lactamase-producing Enterobacterales (ESBL-PE), particularly Escherichia coli and Klebsiella pneumoniae, have been consistently associated with treatment failure, high mortality and morbidity. The emergence of carbapenem resistance among ESBL-PE strains exacerbates the antimicrobial resistance. However, data are very limited in developing countries as Burkina Faso. This study aimed to determine the prevalence of carbapenemase and AmpC-β-lactamase production among ESBL-producing E. coli (ESBL-Ec) and Klebsiella spp. (ESBL-K) isolated from patients’ stool in Burkina Faso. Materials and methods From January 2020 to June 2022, we isolated 277 ESBL-PE from patients’ stool in five hospitals in Burkina Faso. The strains were isolated on ESBL-selective chromogenic media and identified using API20E. The isolates were tested against 15 antimicrobial agents using the disc-diffusion method on Mueller–Hinton (MH) agar. ESBL production was confirmed by double disc synergy method. Carbapenemase and AmpC-β-lactamase production and phenotypic co-resistance were determined. Results Among the 277 ESBL-PE strains isolated, 203 were E. coli, and 74 were Klebsiella spp. Of these bacteria, 2.9% were carbapenemase producers and 6.5% were AmpC-β-lactamase producers. The carbapenemase producers were detected at tertiary and secondary hospitals, mainly in hospitalized patients and females, whereas AmpC-β-lactamase producers were detected at all levels of healthcare, predominantly in non-hospitalized patients, male, and under 15 years of age. The co-resistance rates were as high as 82% for fluoroquinolones, 91% for aminoglycosides, and 94% for sulfonamides. Fosfomycin resistance was 2.5% for ESBL-Ec and 50% for ESBL-K. Conclusion This study showed that ESBL-PEs co-produce carbapenemase and/or AmpC-β-lactamase. High co-resistances were reported for commonly used antibiotic agents. Therefore, screening for carbapenem-resistant Enterobacterales (CRE) carriage is necessary to limit its spread within hospitals. |
| format | Article |
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| institution | Kabale University |
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| spelling | doaj-art-aa8c14cd28b6448b91f04a886ab169092025-01-26T12:16:55ZengBMCBMC Infectious Diseases1471-23342025-01-0125111010.1186/s12879-025-10506-4Fecal carriage of carbapenemase and AmpC-β-lactamase producers among extended spectrum β-Lactamase-producing E. coli and Klebsiella spp. isolates in patients attending hospitalsZakaria Garba0Isidore J. O. Bonkoungou1Namwin Siourimè Somda2Magloire H. Natama3Georges Somé4Lassana Sangaré5Nicolas Barro6Halidou Tinto7Department of Biochemistry and Microbiology, Université Joseph KI-ZERBODepartment of Biochemistry and Microbiology, Université Joseph KI-ZERBODépartement Technologie Alimentaire (DTA), IRSAT / CNRSTClinical Research Unit of Nanoro, Institut de Recherche en Sciences de la SantéClinical Research Unit of Nanoro, Institut de Recherche en Sciences de la SantéDepartment of Health Sciences, Université Joseph KI-ZERBODepartment of Biochemistry and Microbiology, Université Joseph KI-ZERBOClinical Research Unit of Nanoro, Institut de Recherche en Sciences de la SantéAbstract Background Extended-spectrum β-lactamase-producing Enterobacterales (ESBL-PE), particularly Escherichia coli and Klebsiella pneumoniae, have been consistently associated with treatment failure, high mortality and morbidity. The emergence of carbapenem resistance among ESBL-PE strains exacerbates the antimicrobial resistance. However, data are very limited in developing countries as Burkina Faso. This study aimed to determine the prevalence of carbapenemase and AmpC-β-lactamase production among ESBL-producing E. coli (ESBL-Ec) and Klebsiella spp. (ESBL-K) isolated from patients’ stool in Burkina Faso. Materials and methods From January 2020 to June 2022, we isolated 277 ESBL-PE from patients’ stool in five hospitals in Burkina Faso. The strains were isolated on ESBL-selective chromogenic media and identified using API20E. The isolates were tested against 15 antimicrobial agents using the disc-diffusion method on Mueller–Hinton (MH) agar. ESBL production was confirmed by double disc synergy method. Carbapenemase and AmpC-β-lactamase production and phenotypic co-resistance were determined. Results Among the 277 ESBL-PE strains isolated, 203 were E. coli, and 74 were Klebsiella spp. Of these bacteria, 2.9% were carbapenemase producers and 6.5% were AmpC-β-lactamase producers. The carbapenemase producers were detected at tertiary and secondary hospitals, mainly in hospitalized patients and females, whereas AmpC-β-lactamase producers were detected at all levels of healthcare, predominantly in non-hospitalized patients, male, and under 15 years of age. The co-resistance rates were as high as 82% for fluoroquinolones, 91% for aminoglycosides, and 94% for sulfonamides. Fosfomycin resistance was 2.5% for ESBL-Ec and 50% for ESBL-K. Conclusion This study showed that ESBL-PEs co-produce carbapenemase and/or AmpC-β-lactamase. High co-resistances were reported for commonly used antibiotic agents. Therefore, screening for carbapenem-resistant Enterobacterales (CRE) carriage is necessary to limit its spread within hospitals.https://doi.org/10.1186/s12879-025-10506-4ESBL-PEAmpC-β-lactamaseCarbapenemaseE. coliKlebsiella |
| spellingShingle | Zakaria Garba Isidore J. O. Bonkoungou Namwin Siourimè Somda Magloire H. Natama Georges Somé Lassana Sangaré Nicolas Barro Halidou Tinto Fecal carriage of carbapenemase and AmpC-β-lactamase producers among extended spectrum β-Lactamase-producing E. coli and Klebsiella spp. isolates in patients attending hospitals BMC Infectious Diseases ESBL-PE AmpC-β-lactamase Carbapenemase E. coli Klebsiella |
| title | Fecal carriage of carbapenemase and AmpC-β-lactamase producers among extended spectrum β-Lactamase-producing E. coli and Klebsiella spp. isolates in patients attending hospitals |
| title_full | Fecal carriage of carbapenemase and AmpC-β-lactamase producers among extended spectrum β-Lactamase-producing E. coli and Klebsiella spp. isolates in patients attending hospitals |
| title_fullStr | Fecal carriage of carbapenemase and AmpC-β-lactamase producers among extended spectrum β-Lactamase-producing E. coli and Klebsiella spp. isolates in patients attending hospitals |
| title_full_unstemmed | Fecal carriage of carbapenemase and AmpC-β-lactamase producers among extended spectrum β-Lactamase-producing E. coli and Klebsiella spp. isolates in patients attending hospitals |
| title_short | Fecal carriage of carbapenemase and AmpC-β-lactamase producers among extended spectrum β-Lactamase-producing E. coli and Klebsiella spp. isolates in patients attending hospitals |
| title_sort | fecal carriage of carbapenemase and ampc β lactamase producers among extended spectrum β lactamase producing e coli and klebsiella spp isolates in patients attending hospitals |
| topic | ESBL-PE AmpC-β-lactamase Carbapenemase E. coli Klebsiella |
| url | https://doi.org/10.1186/s12879-025-10506-4 |
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