Studies on the Anti-Inflammatory Effect and Its Mechanisms of Sophoridine
This work is to study the anti-inflammatory effect and its mechanisms of sophoridine in vitro and in vivo. For this aim, the influences of sophoridine on several inflammatory mediators were investigated. Excessive inflammatory response in vitro model was developed by using lipopolysaccharide (LPS) t...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2014/502626 |
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| _version_ | 1832568021142470656 |
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| author | Xiumei Huang Bo Li Lianzhong Shen |
| author_facet | Xiumei Huang Bo Li Lianzhong Shen |
| author_sort | Xiumei Huang |
| collection | DOAJ |
| description | This work is to study the anti-inflammatory effect and its mechanisms of sophoridine in vitro and in vivo. For this aim, the influences of sophoridine on several inflammatory mediators were investigated. Excessive inflammatory response in vitro model was developed by using lipopolysaccharide (LPS) to stimulate the mouse peritoneal macrophages and HL-60 cells to produce IL-6 and IL-8. Carrageenin-induced mouse paw edema model was used as inflammatory response in vivo model. MTT method, ultraviolet spectrophotometric method, and radioimmunoassay were used to measure the changes of TNFα, IL-6, PGE2, and IL-8 in in vitro cell culture supernatant or in the local inflammatory exudates. The results showed that sophoridine inhibited the production of IL-8 in in vitro cell culture supernatant and inhibited the production of TNFα, PGE2, and IL-8 in the local inflammatory exudates but had no significant effects on the production of IL-6 in vitro and in vivo. It is demonstrated that sophoridine’s anti-inflammatory effect was due to its ability to inhibit the production of cytokine and inflammatory mediators. |
| format | Article |
| id | doaj-art-aa51021f2d1849678d05df35af72ff64 |
| institution | Kabale University |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-aa51021f2d1849678d05df35af72ff642025-02-03T00:59:56ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732014-01-01201410.1155/2014/502626502626Studies on the Anti-Inflammatory Effect and Its Mechanisms of SophoridineXiumei Huang0Bo Li1Lianzhong Shen2National Institutes for Food and Drug Control, Beijing 100050, ChinaNational Institutes for Food and Drug Control, Beijing 100050, ChinaNational Institutes for Food and Drug Control, Beijing 100050, ChinaThis work is to study the anti-inflammatory effect and its mechanisms of sophoridine in vitro and in vivo. For this aim, the influences of sophoridine on several inflammatory mediators were investigated. Excessive inflammatory response in vitro model was developed by using lipopolysaccharide (LPS) to stimulate the mouse peritoneal macrophages and HL-60 cells to produce IL-6 and IL-8. Carrageenin-induced mouse paw edema model was used as inflammatory response in vivo model. MTT method, ultraviolet spectrophotometric method, and radioimmunoassay were used to measure the changes of TNFα, IL-6, PGE2, and IL-8 in in vitro cell culture supernatant or in the local inflammatory exudates. The results showed that sophoridine inhibited the production of IL-8 in in vitro cell culture supernatant and inhibited the production of TNFα, PGE2, and IL-8 in the local inflammatory exudates but had no significant effects on the production of IL-6 in vitro and in vivo. It is demonstrated that sophoridine’s anti-inflammatory effect was due to its ability to inhibit the production of cytokine and inflammatory mediators.http://dx.doi.org/10.1155/2014/502626 |
| spellingShingle | Xiumei Huang Bo Li Lianzhong Shen Studies on the Anti-Inflammatory Effect and Its Mechanisms of Sophoridine Journal of Analytical Methods in Chemistry |
| title | Studies on the Anti-Inflammatory Effect and Its Mechanisms of Sophoridine |
| title_full | Studies on the Anti-Inflammatory Effect and Its Mechanisms of Sophoridine |
| title_fullStr | Studies on the Anti-Inflammatory Effect and Its Mechanisms of Sophoridine |
| title_full_unstemmed | Studies on the Anti-Inflammatory Effect and Its Mechanisms of Sophoridine |
| title_short | Studies on the Anti-Inflammatory Effect and Its Mechanisms of Sophoridine |
| title_sort | studies on the anti inflammatory effect and its mechanisms of sophoridine |
| url | http://dx.doi.org/10.1155/2014/502626 |
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