A dataset of expression profile of certain microRNAs in Caenorhabditis elegans
Abstract MicroRNAs (miRNAs), as crucial gene regulatory molecules, participate in the regulation of biological processes through multiple mechanisms such as transcriptional repression and mRNA degradation. In this study, we first examined the expression patterns of 36 miRNAs at the L1 stage of Caeno...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-07-01
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| Series: | Scientific Data |
| Online Access: | https://doi.org/10.1038/s41597-025-05486-8 |
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| author | Yimeng Gao Yuchuan Bai Shipeng Hu Zhiju Tao Yiru Jiang Di Zhao |
| author_facet | Yimeng Gao Yuchuan Bai Shipeng Hu Zhiju Tao Yiru Jiang Di Zhao |
| author_sort | Yimeng Gao |
| collection | DOAJ |
| description | Abstract MicroRNAs (miRNAs), as crucial gene regulatory molecules, participate in the regulation of biological processes through multiple mechanisms such as transcriptional repression and mRNA degradation. In this study, we first examined the expression patterns of 36 miRNAs at the L1 stage of Caenorhabditis elegans (C. elegans), revealing that the majority exhibited basal low expression levels. To investigate the evolutionary conservation of miRNAs, we selected Caenorhabditis briggsae (C. briggsae), which shares similar embryonic-to-L1-stage cell lineages with C. elegans but is taxonomically distinct, and constructed 15 miRNA reporter strains for cross-species comparison. Additionally, to elucidate the regulatory role of miRNAs in development, we generated single-gene mutants and multi-gene combinatorial mutants targeting miRNAs specifically expressed in excretory cells and quantified the phenotypic variations in excretory cells across different miRNA mutant combinations. The comprehensive dataset provided in this study will serve as a valuable resource for subsequent miRNA functional studies and candidate gene screening, thereby advancing our understanding of miRNA regulatory networks. |
| format | Article |
| id | doaj-art-aa1ed5f1dd2644fa882ae027770bbbdb |
| institution | DOAJ |
| issn | 2052-4463 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Data |
| spelling | doaj-art-aa1ed5f1dd2644fa882ae027770bbbdb2025-08-20T03:03:23ZengNature PortfolioScientific Data2052-44632025-07-011211710.1038/s41597-025-05486-8A dataset of expression profile of certain microRNAs in Caenorhabditis elegansYimeng Gao0Yuchuan Bai1Shipeng Hu2Zhiju Tao3Yiru Jiang4Di Zhao5Tianjin Key Laboratory of Exercise Physiology and Sports Medicine, Institute of Sport, Exercise & Health, Tianjin University of SportCollege of Life Sciences, Capital Normal UniversityCollege of Life Sciences, Capital Normal UniversityCollege of Life Sciences, Capital Normal UniversityCollege of Life Sciences, Capital Normal UniversityTianjin Key Laboratory of Exercise Physiology and Sports Medicine, Institute of Sport, Exercise & Health, Tianjin University of SportAbstract MicroRNAs (miRNAs), as crucial gene regulatory molecules, participate in the regulation of biological processes through multiple mechanisms such as transcriptional repression and mRNA degradation. In this study, we first examined the expression patterns of 36 miRNAs at the L1 stage of Caenorhabditis elegans (C. elegans), revealing that the majority exhibited basal low expression levels. To investigate the evolutionary conservation of miRNAs, we selected Caenorhabditis briggsae (C. briggsae), which shares similar embryonic-to-L1-stage cell lineages with C. elegans but is taxonomically distinct, and constructed 15 miRNA reporter strains for cross-species comparison. Additionally, to elucidate the regulatory role of miRNAs in development, we generated single-gene mutants and multi-gene combinatorial mutants targeting miRNAs specifically expressed in excretory cells and quantified the phenotypic variations in excretory cells across different miRNA mutant combinations. The comprehensive dataset provided in this study will serve as a valuable resource for subsequent miRNA functional studies and candidate gene screening, thereby advancing our understanding of miRNA regulatory networks.https://doi.org/10.1038/s41597-025-05486-8 |
| spellingShingle | Yimeng Gao Yuchuan Bai Shipeng Hu Zhiju Tao Yiru Jiang Di Zhao A dataset of expression profile of certain microRNAs in Caenorhabditis elegans Scientific Data |
| title | A dataset of expression profile of certain microRNAs in Caenorhabditis elegans |
| title_full | A dataset of expression profile of certain microRNAs in Caenorhabditis elegans |
| title_fullStr | A dataset of expression profile of certain microRNAs in Caenorhabditis elegans |
| title_full_unstemmed | A dataset of expression profile of certain microRNAs in Caenorhabditis elegans |
| title_short | A dataset of expression profile of certain microRNAs in Caenorhabditis elegans |
| title_sort | dataset of expression profile of certain micrornas in caenorhabditis elegans |
| url | https://doi.org/10.1038/s41597-025-05486-8 |
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