Isolation of Bacillus sphaericus from Lombok Island, Indonesia, and Their Toxicity against Anopheles aconitus
Malaria is endemic to Lombok Island, Indonesia. One approach to suppress malaria spread is to eliminate anopheline larvae in their habitat and the environmentally safe agent is bacteria, that is, Bacillus sphaericus. However, there is no information regarding local isolate of B. sphaericus that is t...
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| Language: | English |
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Wiley
2015-01-01
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| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2015/854709 |
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| author | Bambang Fajar Suryadi Bagyo Yanuwiadi Tri Ardyati Suharjono |
| author_facet | Bambang Fajar Suryadi Bagyo Yanuwiadi Tri Ardyati Suharjono |
| author_sort | Bambang Fajar Suryadi |
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| description | Malaria is endemic to Lombok Island, Indonesia. One approach to suppress malaria spread is to eliminate anopheline larvae in their habitat and the environmentally safe agent is bacteria, that is, Bacillus sphaericus. However, there is no information regarding local isolate of B. sphaericus that is toxic to mosquito larvae from Lombok. The aims of the study were to isolate B. sphaericus from soil in areas close to beach surrounding Lombok Island and to test their toxicity against 3rd instar Anopheles aconitus larvae. Soil samples were collected from 20 different sampling locations from Lombok Island and homogenized with sterile physiological salt solution. Suspension was heat-shocked at 80°C for 30 minutes and then spread onto antibiotic-supplemented NYSM solid medium. Colonies grown were characterized and subjected to initial toxicity test against anopheline larvae. Isolates with more than 50% killing percentage were subjected to bioassay testing against anopheline larvae. From 20 locations, 1 isolate showed mild toxicity (namely, isolate MNT) and 2 isolates showed high toxicity (namely, isolates SLG and TJL2) against An. aconitus. Those 3 isolates were potentially useful isolates, as they killed almost all larvae in 24 hours. The discovery of toxic indigenous isolates of B. sphaericus from Lombok Island opens opportunity to develop a biopesticide from local resources. |
| format | Article |
| id | doaj-art-aa0d29d205124c85acac4c2489c61c8a |
| institution | Kabale University |
| issn | 1687-918X 1687-9198 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
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| series | International Journal of Microbiology |
| spelling | doaj-art-aa0d29d205124c85acac4c2489c61c8a2025-02-03T06:46:25ZengWileyInternational Journal of Microbiology1687-918X1687-91982015-01-01201510.1155/2015/854709854709Isolation of Bacillus sphaericus from Lombok Island, Indonesia, and Their Toxicity against Anopheles aconitusBambang Fajar Suryadi0Bagyo Yanuwiadi1Tri Ardyati2Suharjono3Department of Biology, Faculty of Mathematics and Natural Sciences, Mataram University, Mataram 83125, IndonesiaDepartment of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya University, Malang 65145, IndonesiaDepartment of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya University, Malang 65145, IndonesiaDepartment of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya University, Malang 65145, IndonesiaMalaria is endemic to Lombok Island, Indonesia. One approach to suppress malaria spread is to eliminate anopheline larvae in their habitat and the environmentally safe agent is bacteria, that is, Bacillus sphaericus. However, there is no information regarding local isolate of B. sphaericus that is toxic to mosquito larvae from Lombok. The aims of the study were to isolate B. sphaericus from soil in areas close to beach surrounding Lombok Island and to test their toxicity against 3rd instar Anopheles aconitus larvae. Soil samples were collected from 20 different sampling locations from Lombok Island and homogenized with sterile physiological salt solution. Suspension was heat-shocked at 80°C for 30 minutes and then spread onto antibiotic-supplemented NYSM solid medium. Colonies grown were characterized and subjected to initial toxicity test against anopheline larvae. Isolates with more than 50% killing percentage were subjected to bioassay testing against anopheline larvae. From 20 locations, 1 isolate showed mild toxicity (namely, isolate MNT) and 2 isolates showed high toxicity (namely, isolates SLG and TJL2) against An. aconitus. Those 3 isolates were potentially useful isolates, as they killed almost all larvae in 24 hours. The discovery of toxic indigenous isolates of B. sphaericus from Lombok Island opens opportunity to develop a biopesticide from local resources.http://dx.doi.org/10.1155/2015/854709 |
| spellingShingle | Bambang Fajar Suryadi Bagyo Yanuwiadi Tri Ardyati Suharjono Isolation of Bacillus sphaericus from Lombok Island, Indonesia, and Their Toxicity against Anopheles aconitus International Journal of Microbiology |
| title | Isolation of Bacillus sphaericus from Lombok Island, Indonesia, and Their Toxicity against Anopheles aconitus |
| title_full | Isolation of Bacillus sphaericus from Lombok Island, Indonesia, and Their Toxicity against Anopheles aconitus |
| title_fullStr | Isolation of Bacillus sphaericus from Lombok Island, Indonesia, and Their Toxicity against Anopheles aconitus |
| title_full_unstemmed | Isolation of Bacillus sphaericus from Lombok Island, Indonesia, and Their Toxicity against Anopheles aconitus |
| title_short | Isolation of Bacillus sphaericus from Lombok Island, Indonesia, and Their Toxicity against Anopheles aconitus |
| title_sort | isolation of bacillus sphaericus from lombok island indonesia and their toxicity against anopheles aconitus |
| url | http://dx.doi.org/10.1155/2015/854709 |
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