Crosstalk between Substrates and Rho-Associated Kinase Inhibitors in Cryopreservation of Tissue-Engineered Constructs
It is documented that human mesenchymal stem cells (hMSCs) can be differentiated into various types of cells to present a tool for tissue engineering and regenerative medicine. Thus, the preservation of stem cells is a crucial factor for their effective long-term storage that further facilitates the...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2017-01-01
|
| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2017/1380304 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832559366913392640 |
|---|---|
| author | Arindam Bit Awanish Kumar Abhishek Kumar Singh Albert A. Rizvanov Andrey P. Kiassov Pradeep Kumar Patra Munish Kumar Akalabya Bissoyi |
| author_facet | Arindam Bit Awanish Kumar Abhishek Kumar Singh Albert A. Rizvanov Andrey P. Kiassov Pradeep Kumar Patra Munish Kumar Akalabya Bissoyi |
| author_sort | Arindam Bit |
| collection | DOAJ |
| description | It is documented that human mesenchymal stem cells (hMSCs) can be differentiated into various types of cells to present a tool for tissue engineering and regenerative medicine. Thus, the preservation of stem cells is a crucial factor for their effective long-term storage that further facilitates their continuous supply and transportation for application in regenerative medicine. Cryopreservation is the most important, practicable, and the only established mechanism for long-term preservation of cells, tissues, and organs, and engineered tissues; thus, it is the key step for the improvement of tissue engineering. A significant portion of MSCs loses cellular viability while freeze-thawing, which represents an important technical limitation to achieving sufficient viable cell numbers for maximum efficacy. Several natural and synthetic materials are extensively used as substrates for tissue engineering constructs and cryopreservation because they promote cell attachment and proliferation. Rho-associated kinase (ROCK) inhibitors can improve the physiological function and postthaw viability of cryopreserved MSCs. This review proposes a crosstalk between substrate topology and interaction of cells with ROCK inhibitors. It is shown that incorporation of ionic nanoparticles in the presence of an external electrical field improves the generation of ROCK inhibitors to safeguard cellular viability for the enhanced cryopreservation of engineered tissues. |
| format | Article |
| id | doaj-art-a9cdd04bede449fc960ef05a445877b3 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-a9cdd04bede449fc960ef05a445877b32025-02-03T01:30:17ZengWileyStem Cells International1687-966X1687-96782017-01-01201710.1155/2017/13803041380304Crosstalk between Substrates and Rho-Associated Kinase Inhibitors in Cryopreservation of Tissue-Engineered ConstructsArindam Bit0Awanish Kumar1Abhishek Kumar Singh2Albert A. Rizvanov3Andrey P. Kiassov4Pradeep Kumar Patra5Munish Kumar6Akalabya Bissoyi7Department of Biomedical Engineering, National Institute of Technology, Raipur 492010, IndiaDepartment of Biotechnology, National Institute of Technology, Raipur 492010, IndiaDepartment of Biochemistry, University of Allahabad, Allahabad 211002, IndiaInstitute of Fundamental Medicine and Biology, Kazan Federal University, Kazan 420008, RussiaInstitute of Fundamental Medicine and Biology, Kazan Federal University, Kazan 420008, RussiaDepartment of Biochemistry, Pt. JNM Medical College, Raipur 492001, IndiaDepartment of Biochemistry, University of Allahabad, Allahabad 211002, IndiaDepartment of Biomedical Engineering, National Institute of Technology, Raipur 492010, IndiaIt is documented that human mesenchymal stem cells (hMSCs) can be differentiated into various types of cells to present a tool for tissue engineering and regenerative medicine. Thus, the preservation of stem cells is a crucial factor for their effective long-term storage that further facilitates their continuous supply and transportation for application in regenerative medicine. Cryopreservation is the most important, practicable, and the only established mechanism for long-term preservation of cells, tissues, and organs, and engineered tissues; thus, it is the key step for the improvement of tissue engineering. A significant portion of MSCs loses cellular viability while freeze-thawing, which represents an important technical limitation to achieving sufficient viable cell numbers for maximum efficacy. Several natural and synthetic materials are extensively used as substrates for tissue engineering constructs and cryopreservation because they promote cell attachment and proliferation. Rho-associated kinase (ROCK) inhibitors can improve the physiological function and postthaw viability of cryopreserved MSCs. This review proposes a crosstalk between substrate topology and interaction of cells with ROCK inhibitors. It is shown that incorporation of ionic nanoparticles in the presence of an external electrical field improves the generation of ROCK inhibitors to safeguard cellular viability for the enhanced cryopreservation of engineered tissues.http://dx.doi.org/10.1155/2017/1380304 |
| spellingShingle | Arindam Bit Awanish Kumar Abhishek Kumar Singh Albert A. Rizvanov Andrey P. Kiassov Pradeep Kumar Patra Munish Kumar Akalabya Bissoyi Crosstalk between Substrates and Rho-Associated Kinase Inhibitors in Cryopreservation of Tissue-Engineered Constructs Stem Cells International |
| title | Crosstalk between Substrates and Rho-Associated Kinase Inhibitors in Cryopreservation of Tissue-Engineered Constructs |
| title_full | Crosstalk between Substrates and Rho-Associated Kinase Inhibitors in Cryopreservation of Tissue-Engineered Constructs |
| title_fullStr | Crosstalk between Substrates and Rho-Associated Kinase Inhibitors in Cryopreservation of Tissue-Engineered Constructs |
| title_full_unstemmed | Crosstalk between Substrates and Rho-Associated Kinase Inhibitors in Cryopreservation of Tissue-Engineered Constructs |
| title_short | Crosstalk between Substrates and Rho-Associated Kinase Inhibitors in Cryopreservation of Tissue-Engineered Constructs |
| title_sort | crosstalk between substrates and rho associated kinase inhibitors in cryopreservation of tissue engineered constructs |
| url | http://dx.doi.org/10.1155/2017/1380304 |
| work_keys_str_mv | AT arindambit crosstalkbetweensubstratesandrhoassociatedkinaseinhibitorsincryopreservationoftissueengineeredconstructs AT awanishkumar crosstalkbetweensubstratesandrhoassociatedkinaseinhibitorsincryopreservationoftissueengineeredconstructs AT abhishekkumarsingh crosstalkbetweensubstratesandrhoassociatedkinaseinhibitorsincryopreservationoftissueengineeredconstructs AT albertarizvanov crosstalkbetweensubstratesandrhoassociatedkinaseinhibitorsincryopreservationoftissueengineeredconstructs AT andreypkiassov crosstalkbetweensubstratesandrhoassociatedkinaseinhibitorsincryopreservationoftissueengineeredconstructs AT pradeepkumarpatra crosstalkbetweensubstratesandrhoassociatedkinaseinhibitorsincryopreservationoftissueengineeredconstructs AT munishkumar crosstalkbetweensubstratesandrhoassociatedkinaseinhibitorsincryopreservationoftissueengineeredconstructs AT akalabyabissoyi crosstalkbetweensubstratesandrhoassociatedkinaseinhibitorsincryopreservationoftissueengineeredconstructs |