Bioactive Molecules from the Invasive Blue Crab <i>Callinectes sapidus</i> Exoskeleton: Evaluation of Reducing, Radical Scavenging, and Antitumor Activities
In recent years, the invasive Atlantic blue crab (<i>Callinectes sapidus</i>) has increased its spread throughout the Mediterranean Sea, threatening native biodiversity and local economies. This study aimed to valorize <i>C. sapidus</i> sampled in Sicily by utilizing its exos...
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MDPI AG
2025-01-01
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| Series: | Marine Drugs |
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| author | Francesco Longo Alessandro Attanzio Laura Marretta Claudio Luparello Serena Indelicato David Bongiorno Giampaolo Barone Luisa Tesoriere Ilenia Concetta Giardina Giulia Abruscato Manuela Perlotti Lucie Branwen Hornsby Vincenzo Arizza Mirella Vazzana Aiti Vizzini Chiara Martino Angelica Listro Vinicius Queiroz Antonio Fabbrizio Paolo Salvatore Francesco Ciaccio Stella Maria Cascioferro Francesca Di Gaudio Manuela Mauro |
| author_facet | Francesco Longo Alessandro Attanzio Laura Marretta Claudio Luparello Serena Indelicato David Bongiorno Giampaolo Barone Luisa Tesoriere Ilenia Concetta Giardina Giulia Abruscato Manuela Perlotti Lucie Branwen Hornsby Vincenzo Arizza Mirella Vazzana Aiti Vizzini Chiara Martino Angelica Listro Vinicius Queiroz Antonio Fabbrizio Paolo Salvatore Francesco Ciaccio Stella Maria Cascioferro Francesca Di Gaudio Manuela Mauro |
| author_sort | Francesco Longo |
| collection | DOAJ |
| description | In recent years, the invasive Atlantic blue crab (<i>Callinectes sapidus</i>) has increased its spread throughout the Mediterranean Sea, threatening native biodiversity and local economies. This study aimed to valorize <i>C. sapidus</i> sampled in Sicily by utilizing its exoskeleton as a source of chitosan, astaxanthin, and bio-phenolic compounds. These biomolecules were evaluated for their reducing, radical scavenging, and antitumor activity. The ferric ion reducing antioxidant power (FRAP) and the free radical scavenging activity against radical 2,2-Diphenyl-1-picrylhydrazyl (DPPH) were significantly higher for chitosan (3.16 ± 0.10 mg AAE/g and 8.1 ± 0.10 µmol TE/g). No significant differences were observed among the tested biomolecules in their activity in scavenging the radical 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS). Both bio-phenolic compounds and astaxanthin exhibited dose-dependent cytotoxicity on CaCo-2 (IC<sub>50</sub> = 12.47 and 18 µg/mL) and HepG2 (IC<sub>50</sub> = 10.25 and 1.26 µg/mL) cell lines, while only bio-phenols showed no cytotoxic effect on differentiated CaCo-2 cells up to 20 µg/mL. These findings highlight the value of blue crab by-products in supporting a circular economy, offering a sustainable approach to managing this invasive species while providing bioactive compounds with promising medical and nutraceutical applications. |
| format | Article |
| id | doaj-art-a9cc9aa258cd466295b65496eaa69e84 |
| institution | Kabale University |
| issn | 1660-3397 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj-art-a9cc9aa258cd466295b65496eaa69e842025-01-24T13:39:37ZengMDPI AGMarine Drugs1660-33972025-01-012314510.3390/md23010045Bioactive Molecules from the Invasive Blue Crab <i>Callinectes sapidus</i> Exoskeleton: Evaluation of Reducing, Radical Scavenging, and Antitumor ActivitiesFrancesco Longo0Alessandro Attanzio1Laura Marretta2Claudio Luparello3Serena Indelicato4David Bongiorno5Giampaolo Barone6Luisa Tesoriere7Ilenia Concetta Giardina8Giulia Abruscato9Manuela Perlotti10Lucie Branwen Hornsby11Vincenzo Arizza12Mirella Vazzana13Aiti Vizzini14Chiara Martino15Angelica Listro16Vinicius Queiroz17Antonio Fabbrizio18Paolo Salvatore Francesco Ciaccio19Stella Maria Cascioferro20Francesca Di Gaudio21Manuela Mauro22Department of Biological, Chemical and Pharmaceutical Sciences and Technologies (STEBICEF), University of Palermo, 90123 Palermo, ItalyDepartment of Biological, Chemical and Pharmaceutical Sciences and Technologies (STEBICEF), University of Palermo, 90123 Palermo, ItalyDepartment of Biological, Chemical and Pharmaceutical Sciences and Technologies (STEBICEF), University of Palermo, 90123 Palermo, ItalyDepartment of Biological, Chemical and Pharmaceutical Sciences and Technologies (STEBICEF), University of Palermo, 90123 Palermo, ItalyDepartment of Biological, Chemical and Pharmaceutical Sciences and Technologies (STEBICEF), University of Palermo, 90123 Palermo, ItalyDepartment of Biological, Chemical and Pharmaceutical Sciences and Technologies (STEBICEF), University of Palermo, 90123 Palermo, ItalyDepartment of Biological, Chemical and Pharmaceutical Sciences and Technologies (STEBICEF), University of Palermo, 90123 Palermo, ItalyDepartment of Biological, Chemical and Pharmaceutical Sciences and Technologies (STEBICEF), University of Palermo, 90123 Palermo, ItalyDepartment of Biological, Chemical and Pharmaceutical Sciences and Technologies (STEBICEF), University of Palermo, 90123 Palermo, ItalyDepartment of Biological, Chemical and Pharmaceutical Sciences and Technologies (STEBICEF), University of Palermo, 90123 Palermo, ItalyDepartment of Biological, Chemical and Pharmaceutical Sciences and Technologies (STEBICEF), University of Palermo, 90123 Palermo, ItalyDepartment of Biological, Chemical and Pharmaceutical Sciences and Technologies (STEBICEF), University of Palermo, 90123 Palermo, ItalyDepartment of Biological, Chemical and Pharmaceutical Sciences and Technologies (STEBICEF), University of Palermo, 90123 Palermo, ItalyDepartment of Biological, Chemical and Pharmaceutical Sciences and Technologies (STEBICEF), University of Palermo, 90123 Palermo, ItalyDepartment of Biological, Chemical and Pharmaceutical Sciences and Technologies (STEBICEF), University of Palermo, 90123 Palermo, ItalyDepartment of Biological, Chemical and Pharmaceutical Sciences and Technologies (STEBICEF), University of Palermo, 90123 Palermo, ItalyDepartment of Biological, Chemical and Pharmaceutical Sciences and Technologies (STEBICEF), University of Palermo, 90123 Palermo, ItalyDepartamento de Fisiologia, Instituto de Biociências, Universidade de São Paulo, Sao Paulo 05508-090, BrazilDepartment of Theoretical and Applied Sciences (DiSTA), University e Campus, 22060 Novedrate, ItalyIndependent Researcher, 92019 Sciacca, ItalyDepartment of Biological, Chemical and Pharmaceutical Sciences and Technologies (STEBICEF), University of Palermo, 90123 Palermo, ItalyDepartment PROMISE, University of Palermo, Piazza delle Cliniche, 2, 90127 Palermo, ItalyDepartment of Biological, Chemical and Pharmaceutical Sciences and Technologies (STEBICEF), University of Palermo, 90123 Palermo, ItalyIn recent years, the invasive Atlantic blue crab (<i>Callinectes sapidus</i>) has increased its spread throughout the Mediterranean Sea, threatening native biodiversity and local economies. This study aimed to valorize <i>C. sapidus</i> sampled in Sicily by utilizing its exoskeleton as a source of chitosan, astaxanthin, and bio-phenolic compounds. These biomolecules were evaluated for their reducing, radical scavenging, and antitumor activity. The ferric ion reducing antioxidant power (FRAP) and the free radical scavenging activity against radical 2,2-Diphenyl-1-picrylhydrazyl (DPPH) were significantly higher for chitosan (3.16 ± 0.10 mg AAE/g and 8.1 ± 0.10 µmol TE/g). No significant differences were observed among the tested biomolecules in their activity in scavenging the radical 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS). Both bio-phenolic compounds and astaxanthin exhibited dose-dependent cytotoxicity on CaCo-2 (IC<sub>50</sub> = 12.47 and 18 µg/mL) and HepG2 (IC<sub>50</sub> = 10.25 and 1.26 µg/mL) cell lines, while only bio-phenols showed no cytotoxic effect on differentiated CaCo-2 cells up to 20 µg/mL. These findings highlight the value of blue crab by-products in supporting a circular economy, offering a sustainable approach to managing this invasive species while providing bioactive compounds with promising medical and nutraceutical applications.https://www.mdpi.com/1660-3397/23/1/45Atlantic blue crabcrustaceansexoskeletonchitosanastaxanthinpolyphenols |
| spellingShingle | Francesco Longo Alessandro Attanzio Laura Marretta Claudio Luparello Serena Indelicato David Bongiorno Giampaolo Barone Luisa Tesoriere Ilenia Concetta Giardina Giulia Abruscato Manuela Perlotti Lucie Branwen Hornsby Vincenzo Arizza Mirella Vazzana Aiti Vizzini Chiara Martino Angelica Listro Vinicius Queiroz Antonio Fabbrizio Paolo Salvatore Francesco Ciaccio Stella Maria Cascioferro Francesca Di Gaudio Manuela Mauro Bioactive Molecules from the Invasive Blue Crab <i>Callinectes sapidus</i> Exoskeleton: Evaluation of Reducing, Radical Scavenging, and Antitumor Activities Marine Drugs Atlantic blue crab crustaceans exoskeleton chitosan astaxanthin polyphenols |
| title | Bioactive Molecules from the Invasive Blue Crab <i>Callinectes sapidus</i> Exoskeleton: Evaluation of Reducing, Radical Scavenging, and Antitumor Activities |
| title_full | Bioactive Molecules from the Invasive Blue Crab <i>Callinectes sapidus</i> Exoskeleton: Evaluation of Reducing, Radical Scavenging, and Antitumor Activities |
| title_fullStr | Bioactive Molecules from the Invasive Blue Crab <i>Callinectes sapidus</i> Exoskeleton: Evaluation of Reducing, Radical Scavenging, and Antitumor Activities |
| title_full_unstemmed | Bioactive Molecules from the Invasive Blue Crab <i>Callinectes sapidus</i> Exoskeleton: Evaluation of Reducing, Radical Scavenging, and Antitumor Activities |
| title_short | Bioactive Molecules from the Invasive Blue Crab <i>Callinectes sapidus</i> Exoskeleton: Evaluation of Reducing, Radical Scavenging, and Antitumor Activities |
| title_sort | bioactive molecules from the invasive blue crab i callinectes sapidus i exoskeleton evaluation of reducing radical scavenging and antitumor activities |
| topic | Atlantic blue crab crustaceans exoskeleton chitosan astaxanthin polyphenols |
| url | https://www.mdpi.com/1660-3397/23/1/45 |
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