Urothelial Differentiation of Human Umbilical Cord-Derived Mesenchymal Stromal Cells In Vitro
Human umbilical cord-derived mesenchymal stromal cells (hUCMSCs) are the most primitive of those isolated from other post-natal tissue source. The hUCMSCs possess the capability of differentiating along multi-lineage. This study aimed to investigate whether hUCMSCs can differentiate into urothelium-...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Analytical Cellular Pathology |
| Online Access: | http://dx.doi.org/10.3233/ACP-130081 |
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| author | Shuai Wu Zhongliang Cheng Guohua Liu Xinfeng Zhao Liang Zhong Yingjian Zhu Jiang Zhu |
| author_facet | Shuai Wu Zhongliang Cheng Guohua Liu Xinfeng Zhao Liang Zhong Yingjian Zhu Jiang Zhu |
| author_sort | Shuai Wu |
| collection | DOAJ |
| description | Human umbilical cord-derived mesenchymal stromal cells (hUCMSCs) are the most primitive of those isolated from other post-natal tissue source. The hUCMSCs possess the capability of differentiating along multi-lineage. This study aimed to investigate whether hUCMSCs can differentiate into urothelium-like cells. The hUCMSCs were isolated from fresh human umbilical cord postpartum and expanded at least to passage 3 in vitro. Subsequently, they were cultured with conditioned medium from urothelial cells (UC-CM) supplemented with 20 ng/ml exogenous epidermal growth factor (EGF). Urothelial cell specific marker uroplakin II (UPII) and cytokeratins were evaluated by reverse transcriptase-polymerase chain reaction (RT-PCR) and immunofluorescence technology. During culture, hUCMSCs started to express UPII and cytokeratins weakly at 7 days and were significantly up-regulated at 2 weeks post-induction. Additionally, morphology of hUCMSCs changed from spindle-shape to a polygonal epithelial-shape similar to that of urothelial cells after 7 days. The study results indicated that hUCMSCs can differentiate into urothelium-like cells in a defined micro-environment in vitro constituted by UC-CM and exogenous EGF. |
| format | Article |
| id | doaj-art-a9c115cc032840d08300e84a9b0e26e9 |
| institution | Kabale University |
| issn | 2210-7177 2210-7185 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Analytical Cellular Pathology |
| spelling | doaj-art-a9c115cc032840d08300e84a9b0e26e92025-02-03T06:45:57ZengWileyAnalytical Cellular Pathology2210-71772210-71852013-01-01363-4636910.3233/ACP-130081Urothelial Differentiation of Human Umbilical Cord-Derived Mesenchymal Stromal Cells In VitroShuai Wu0Zhongliang Cheng1Guohua Liu2Xinfeng Zhao3Liang Zhong4Yingjian Zhu5Jiang Zhu6School of Medicine, Shanghai First People's Hospital, Shanghai Jiao Tong University, Shanghai, ChinaSchool of Medicine, Shanghai First People's Hospital, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Urology, Shanghai First People's Hospital, Shanghai Jiao Tong University, Shanghai, ChinaSection of Animal Models for Human Disease, Shanghai First People's Hospital, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Urology, Shanghai First People's Hospital, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Urology, Shanghai First People's Hospital, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Urology, Shanghai First People's Hospital, Shanghai Jiao Tong University, Shanghai, ChinaHuman umbilical cord-derived mesenchymal stromal cells (hUCMSCs) are the most primitive of those isolated from other post-natal tissue source. The hUCMSCs possess the capability of differentiating along multi-lineage. This study aimed to investigate whether hUCMSCs can differentiate into urothelium-like cells. The hUCMSCs were isolated from fresh human umbilical cord postpartum and expanded at least to passage 3 in vitro. Subsequently, they were cultured with conditioned medium from urothelial cells (UC-CM) supplemented with 20 ng/ml exogenous epidermal growth factor (EGF). Urothelial cell specific marker uroplakin II (UPII) and cytokeratins were evaluated by reverse transcriptase-polymerase chain reaction (RT-PCR) and immunofluorescence technology. During culture, hUCMSCs started to express UPII and cytokeratins weakly at 7 days and were significantly up-regulated at 2 weeks post-induction. Additionally, morphology of hUCMSCs changed from spindle-shape to a polygonal epithelial-shape similar to that of urothelial cells after 7 days. The study results indicated that hUCMSCs can differentiate into urothelium-like cells in a defined micro-environment in vitro constituted by UC-CM and exogenous EGF.http://dx.doi.org/10.3233/ACP-130081 |
| spellingShingle | Shuai Wu Zhongliang Cheng Guohua Liu Xinfeng Zhao Liang Zhong Yingjian Zhu Jiang Zhu Urothelial Differentiation of Human Umbilical Cord-Derived Mesenchymal Stromal Cells In Vitro Analytical Cellular Pathology |
| title | Urothelial Differentiation of Human Umbilical Cord-Derived Mesenchymal Stromal Cells In Vitro |
| title_full | Urothelial Differentiation of Human Umbilical Cord-Derived Mesenchymal Stromal Cells In Vitro |
| title_fullStr | Urothelial Differentiation of Human Umbilical Cord-Derived Mesenchymal Stromal Cells In Vitro |
| title_full_unstemmed | Urothelial Differentiation of Human Umbilical Cord-Derived Mesenchymal Stromal Cells In Vitro |
| title_short | Urothelial Differentiation of Human Umbilical Cord-Derived Mesenchymal Stromal Cells In Vitro |
| title_sort | urothelial differentiation of human umbilical cord derived mesenchymal stromal cells in vitro |
| url | http://dx.doi.org/10.3233/ACP-130081 |
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