Evaluation of the Utility of the Random Amplified Polymorphic DNA Method and of the Semi-Specific PCR to Assess the Genetic Diversification of the Gerbera jamesonii Bolus Line
An attempt was made to evaluate the utility of a method which employs semi-specific PCR using partially specific primers for the coding sequence (ET) at the exon-intron contact and of the RAPD method to identify eight Polish cultivars of gerbera. It was demonstrated that the PCR method which employs...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/2012/450920 |
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| author | Zbigniew Rusinowski Olga Domeradzka |
| author_facet | Zbigniew Rusinowski Olga Domeradzka |
| author_sort | Zbigniew Rusinowski |
| collection | DOAJ |
| description | An attempt was made to evaluate the utility of a method which employs semi-specific PCR using partially specific primers for the coding sequence (ET) at the exon-intron contact and of the RAPD method to identify eight Polish cultivars of gerbera. It was demonstrated that the PCR method which employs semi-specific primers is as simple and economical as the RAPD method, simultaneously the images of the multiplied by means of the semi-specific PCR method DNA fragments are more complex and polymorphic than those obtained through the RAPD method. The studies of the genetic diversification of Gerbera cultivars employing the aforementioned methods made it possible to conduct a concentration analysis and evaluation of the genetic distance between the lines, manifesting at the same time the superiority of the semi-random PCR method. Moreover, it transpired that the use of mixtures of RAPD primers not always leads to an increase of the number of generated polymorphic bands. |
| format | Article |
| id | doaj-art-a9b883960d9945048203003a858ee9c5 |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-a9b883960d9945048203003a858ee9c52025-02-03T05:50:44ZengWileyThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/450920450920Evaluation of the Utility of the Random Amplified Polymorphic DNA Method and of the Semi-Specific PCR to Assess the Genetic Diversification of the Gerbera jamesonii Bolus LineZbigniew Rusinowski0Olga Domeradzka1Department of Ornamental Plants, Warsaw School of Life Sciences, Faculty of Horticulture and Landscape Architecture, Warsaw University of Life Sciences, Nowoursynowska 159, 02776 Warsaw, PolandDepartment of Ornamental Plants, Warsaw School of Life Sciences, Faculty of Horticulture and Landscape Architecture, Warsaw University of Life Sciences, Nowoursynowska 159, 02776 Warsaw, PolandAn attempt was made to evaluate the utility of a method which employs semi-specific PCR using partially specific primers for the coding sequence (ET) at the exon-intron contact and of the RAPD method to identify eight Polish cultivars of gerbera. It was demonstrated that the PCR method which employs semi-specific primers is as simple and economical as the RAPD method, simultaneously the images of the multiplied by means of the semi-specific PCR method DNA fragments are more complex and polymorphic than those obtained through the RAPD method. The studies of the genetic diversification of Gerbera cultivars employing the aforementioned methods made it possible to conduct a concentration analysis and evaluation of the genetic distance between the lines, manifesting at the same time the superiority of the semi-random PCR method. Moreover, it transpired that the use of mixtures of RAPD primers not always leads to an increase of the number of generated polymorphic bands.http://dx.doi.org/10.1100/2012/450920 |
| spellingShingle | Zbigniew Rusinowski Olga Domeradzka Evaluation of the Utility of the Random Amplified Polymorphic DNA Method and of the Semi-Specific PCR to Assess the Genetic Diversification of the Gerbera jamesonii Bolus Line The Scientific World Journal |
| title | Evaluation of the Utility of the Random Amplified Polymorphic DNA Method and of the Semi-Specific PCR to Assess the Genetic Diversification of the Gerbera jamesonii Bolus Line |
| title_full | Evaluation of the Utility of the Random Amplified Polymorphic DNA Method and of the Semi-Specific PCR to Assess the Genetic Diversification of the Gerbera jamesonii Bolus Line |
| title_fullStr | Evaluation of the Utility of the Random Amplified Polymorphic DNA Method and of the Semi-Specific PCR to Assess the Genetic Diversification of the Gerbera jamesonii Bolus Line |
| title_full_unstemmed | Evaluation of the Utility of the Random Amplified Polymorphic DNA Method and of the Semi-Specific PCR to Assess the Genetic Diversification of the Gerbera jamesonii Bolus Line |
| title_short | Evaluation of the Utility of the Random Amplified Polymorphic DNA Method and of the Semi-Specific PCR to Assess the Genetic Diversification of the Gerbera jamesonii Bolus Line |
| title_sort | evaluation of the utility of the random amplified polymorphic dna method and of the semi specific pcr to assess the genetic diversification of the gerbera jamesonii bolus line |
| url | http://dx.doi.org/10.1100/2012/450920 |
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