Climate, diet, and nutrition drive gut microbiome variation in a fruit-specialist primate

Abstract Much of what we know about drivers of mammalian gut microbiome (GM) variation focuses on limited seasonal data, or effects of dietary fiber, particularly in leaf-eating and grazing taxa. We know little about the synergistic relationships between climate, diet, nutrition, and GM dynamics in...

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Main Authors: Nina Beeby, Lahitsara Jean Pierre, Razafindraibe Faustin Jean Guy, Rakotonjatovo Justin, Tombotiana Aimé Victor, Giulia Rossi, Lotte van den Hout, Jessica M. Rothman, Katherine R. Amato, Timothy H. Webster, Andrea L. Baden
Format: Article
Language:English
Published: Nature Portfolio 2025-07-01
Series:Scientific Reports
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Online Access:https://doi.org/10.1038/s41598-025-07399-3
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author Nina Beeby
Lahitsara Jean Pierre
Razafindraibe Faustin Jean Guy
Rakotonjatovo Justin
Tombotiana Aimé Victor
Giulia Rossi
Lotte van den Hout
Jessica M. Rothman
Katherine R. Amato
Timothy H. Webster
Andrea L. Baden
author_facet Nina Beeby
Lahitsara Jean Pierre
Razafindraibe Faustin Jean Guy
Rakotonjatovo Justin
Tombotiana Aimé Victor
Giulia Rossi
Lotte van den Hout
Jessica M. Rothman
Katherine R. Amato
Timothy H. Webster
Andrea L. Baden
author_sort Nina Beeby
collection DOAJ
description Abstract Much of what we know about drivers of mammalian gut microbiome (GM) variation focuses on limited seasonal data, or effects of dietary fiber, particularly in leaf-eating and grazing taxa. We know little about the synergistic relationships between climate, diet, nutrition, and GM dynamics in wild mammals—particularly in fruit-eating taxa. Here, we examined GM variation across 12 months in a fruit-specialist primate, the black-and-white ruffed lemur (Varecia variegata), which is known to experience substantial environmental variation in its rainforest habitat in Madagascar. We used mixed modeling approaches to estimate the effects of climate, diet, and nutrient intakes on GM alpha diversity and differential abundances. We found substantial intra- and inter-individual GM variation. Climate and nutrient intakes impacted GM alpha diversity, and in addition to degree of frugivory and dietary diversity, each drove changes in differential abundance of unique combinations of microbial taxa. The degree of frugivory predicted few microbial abundances while nutrient intakes predicted a wide diversity, with fibers and non-structural carbohydrates showing inverse patterns to those of fat, indicating that nutrients are more important in driving the GM than simply the food types consumed. These results highlight how physiological flexibility facilitated by GM plasticity may be key to fruit-specialists’ survival of fruit scarcity.
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spelling doaj-art-a9a25be10eea44e2b34e5ba79f5c66232025-08-20T03:37:20ZengNature PortfolioScientific Reports2045-23222025-07-0115111410.1038/s41598-025-07399-3Climate, diet, and nutrition drive gut microbiome variation in a fruit-specialist primateNina Beeby0Lahitsara Jean Pierre1Razafindraibe Faustin Jean Guy2Rakotonjatovo Justin3Tombotiana Aimé Victor4Giulia Rossi5Lotte van den Hout6Jessica M. Rothman7Katherine R. Amato8Timothy H. Webster9Andrea L. Baden10Department of Anthropology, Hunter College of the City University of New YorkRanomafana Ruffed Lemur Project (RRLP)Ranomafana Ruffed Lemur Project (RRLP)Ranomafana Ruffed Lemur Project (RRLP)Ranomafana Ruffed Lemur Project (RRLP)Ranomafana Ruffed Lemur Project (RRLP)Ranomafana Ruffed Lemur Project (RRLP)Department of Anthropology, Hunter College of the City University of New YorkDepartment of Anthropology, Northwestern UniversityDepartment of Anthropology, University of UtahDepartment of Anthropology, Hunter College of the City University of New YorkAbstract Much of what we know about drivers of mammalian gut microbiome (GM) variation focuses on limited seasonal data, or effects of dietary fiber, particularly in leaf-eating and grazing taxa. We know little about the synergistic relationships between climate, diet, nutrition, and GM dynamics in wild mammals—particularly in fruit-eating taxa. Here, we examined GM variation across 12 months in a fruit-specialist primate, the black-and-white ruffed lemur (Varecia variegata), which is known to experience substantial environmental variation in its rainforest habitat in Madagascar. We used mixed modeling approaches to estimate the effects of climate, diet, and nutrient intakes on GM alpha diversity and differential abundances. We found substantial intra- and inter-individual GM variation. Climate and nutrient intakes impacted GM alpha diversity, and in addition to degree of frugivory and dietary diversity, each drove changes in differential abundance of unique combinations of microbial taxa. The degree of frugivory predicted few microbial abundances while nutrient intakes predicted a wide diversity, with fibers and non-structural carbohydrates showing inverse patterns to those of fat, indicating that nutrients are more important in driving the GM than simply the food types consumed. These results highlight how physiological flexibility facilitated by GM plasticity may be key to fruit-specialists’ survival of fruit scarcity.https://doi.org/10.1038/s41598-025-07399-3Nutrient intakesMacronutrientsGut microbiotaFrugivoryLemur
spellingShingle Nina Beeby
Lahitsara Jean Pierre
Razafindraibe Faustin Jean Guy
Rakotonjatovo Justin
Tombotiana Aimé Victor
Giulia Rossi
Lotte van den Hout
Jessica M. Rothman
Katherine R. Amato
Timothy H. Webster
Andrea L. Baden
Climate, diet, and nutrition drive gut microbiome variation in a fruit-specialist primate
Scientific Reports
Nutrient intakes
Macronutrients
Gut microbiota
Frugivory
Lemur
title Climate, diet, and nutrition drive gut microbiome variation in a fruit-specialist primate
title_full Climate, diet, and nutrition drive gut microbiome variation in a fruit-specialist primate
title_fullStr Climate, diet, and nutrition drive gut microbiome variation in a fruit-specialist primate
title_full_unstemmed Climate, diet, and nutrition drive gut microbiome variation in a fruit-specialist primate
title_short Climate, diet, and nutrition drive gut microbiome variation in a fruit-specialist primate
title_sort climate diet and nutrition drive gut microbiome variation in a fruit specialist primate
topic Nutrient intakes
Macronutrients
Gut microbiota
Frugivory
Lemur
url https://doi.org/10.1038/s41598-025-07399-3
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