Intraperitoneal Injection of Multiplacentas Pooled Cells Treatment on a Mouse Model with Aplastic Anemia
Coinfusion of hematopoietic and mesenchymal stem cells is more effective than hematopoietic stem cell transplantation alone. It is necessary to explore a safe and routine mixed stem cell intraperitoneal transplantation method. Multiplacentas pooled cells were intraperitoneally injected into a radiat...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2016/3279793 |
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| author | Jun Li Hong Chen Yan-Bo Lv Qiang Wang Zheng-Jun Xie Li-Hua Ma Jie He Wei Xue Shan Yu Jun Guo Ting-Hua Wang Tian-Xi Wu Xing-Hua Pan |
| author_facet | Jun Li Hong Chen Yan-Bo Lv Qiang Wang Zheng-Jun Xie Li-Hua Ma Jie He Wei Xue Shan Yu Jun Guo Ting-Hua Wang Tian-Xi Wu Xing-Hua Pan |
| author_sort | Jun Li |
| collection | DOAJ |
| description | Coinfusion of hematopoietic and mesenchymal stem cells is more effective than hematopoietic stem cell transplantation alone. It is necessary to explore a safe and routine mixed stem cell intraperitoneal transplantation method. Multiplacentas pooled cells were intraperitoneally injected into a radiation- and immunity-induced mouse aplastic anemia model with single time. Then, mouse survival time, peripheral blood hemoglobin count, bone marrow architecture, and donor cell engraftment were assessed. The recipient mouse exhibited donor cell engraftment in both bone marrow and peripheral blood. Survival time and peripheral blood hemoglobin count increased in placenta pooled cells treated mice, compared with model-only controls (P=0.048 and P=0.000, resp.). However, placentas pooled cells failed to cause a significant decrease in bone marrow pimelosis area (P=0.357). Intraperitoneally transplanted multiplacentas pooled cells can survive and engraft into a host body through blood circulation, which can increase the life span of an aplastic anemia model mice, and delay but not abrogate the development of aplastic anemia. Furthermore, they appear to play a role in increasing peripheral blood hemoglobin level response for increasing the life span of aplastic anemia model mice. |
| format | Article |
| id | doaj-art-a998b14a949148adade139274f818daa |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-a998b14a949148adade139274f818daa2025-02-03T01:10:09ZengWileyStem Cells International1687-966X1687-96782016-01-01201610.1155/2016/32797933279793Intraperitoneal Injection of Multiplacentas Pooled Cells Treatment on a Mouse Model with Aplastic AnemiaJun Li0Hong Chen1Yan-Bo Lv2Qiang Wang3Zheng-Jun Xie4Li-Hua Ma5Jie He6Wei Xue7Shan Yu8Jun Guo9Ting-Hua Wang10Tian-Xi Wu11Xing-Hua Pan12Medical School, Kunming University, Kunming 650214, ChinaDepartment of Oncology, Kunming General Hospital of Chengdu Military Command, Kunming 650032, ChinaStem Cell, Tissue and Organ Engineering Research Center, Kunming General Hospital of Chengdu Military Command, Kunming 650032, ChinaStem Cell, Tissue and Organ Engineering Research Center, Kunming General Hospital of Chengdu Military Command, Kunming 650032, ChinaDepartment of Haematology and Endocrinology, Kunming General Hospital of Chengdu Military Command, Kunming 650032, ChinaStem Cell, Tissue and Organ Engineering Research Center, Kunming General Hospital of Chengdu Military Command, Kunming 650032, ChinaStem Cell, Tissue and Organ Engineering Research Center, Kunming General Hospital of Chengdu Military Command, Kunming 650032, ChinaMedical School, Kunming University, Kunming 650214, ChinaMedical School, Kunming University, Kunming 650214, ChinaDigestive System Department, Kunming General Hospital of Chengdu Military Command, Kunming 650032, ChinaNeuroscience Institute of Kunming Medical University, Kunming 650500, ChinaMedical School, Kunming University, Kunming 650214, ChinaStem Cell, Tissue and Organ Engineering Research Center, Kunming General Hospital of Chengdu Military Command, Kunming 650032, ChinaCoinfusion of hematopoietic and mesenchymal stem cells is more effective than hematopoietic stem cell transplantation alone. It is necessary to explore a safe and routine mixed stem cell intraperitoneal transplantation method. Multiplacentas pooled cells were intraperitoneally injected into a radiation- and immunity-induced mouse aplastic anemia model with single time. Then, mouse survival time, peripheral blood hemoglobin count, bone marrow architecture, and donor cell engraftment were assessed. The recipient mouse exhibited donor cell engraftment in both bone marrow and peripheral blood. Survival time and peripheral blood hemoglobin count increased in placenta pooled cells treated mice, compared with model-only controls (P=0.048 and P=0.000, resp.). However, placentas pooled cells failed to cause a significant decrease in bone marrow pimelosis area (P=0.357). Intraperitoneally transplanted multiplacentas pooled cells can survive and engraft into a host body through blood circulation, which can increase the life span of an aplastic anemia model mice, and delay but not abrogate the development of aplastic anemia. Furthermore, they appear to play a role in increasing peripheral blood hemoglobin level response for increasing the life span of aplastic anemia model mice.http://dx.doi.org/10.1155/2016/3279793 |
| spellingShingle | Jun Li Hong Chen Yan-Bo Lv Qiang Wang Zheng-Jun Xie Li-Hua Ma Jie He Wei Xue Shan Yu Jun Guo Ting-Hua Wang Tian-Xi Wu Xing-Hua Pan Intraperitoneal Injection of Multiplacentas Pooled Cells Treatment on a Mouse Model with Aplastic Anemia Stem Cells International |
| title | Intraperitoneal Injection of Multiplacentas Pooled Cells Treatment on a Mouse Model with Aplastic Anemia |
| title_full | Intraperitoneal Injection of Multiplacentas Pooled Cells Treatment on a Mouse Model with Aplastic Anemia |
| title_fullStr | Intraperitoneal Injection of Multiplacentas Pooled Cells Treatment on a Mouse Model with Aplastic Anemia |
| title_full_unstemmed | Intraperitoneal Injection of Multiplacentas Pooled Cells Treatment on a Mouse Model with Aplastic Anemia |
| title_short | Intraperitoneal Injection of Multiplacentas Pooled Cells Treatment on a Mouse Model with Aplastic Anemia |
| title_sort | intraperitoneal injection of multiplacentas pooled cells treatment on a mouse model with aplastic anemia |
| url | http://dx.doi.org/10.1155/2016/3279793 |
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