Determination of Benzalkonium Chloride in a Disinfectant by UV Spectrophotometry and Gas and High-Performance Liquid Chromatography: Validation, Comparison of Characteristics, and Economic Feasibility
Simple, fast, and validated UV-spectrophotometric, HPLC, and GC methods for the analysis of benzalkonium chloride in a disinfectant were developed. UV-spectrophotometric determination was based on measuring the amount of light absorption of aqueous solutions of benzalkonium chloride at 268 nm. HPLC...
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | International Journal of Analytical Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2022/2932634 |
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| author | Mariia Smolinska Roman Ostapiv Mariia Yurkevych Liubov Poliuzhyn Olha Korobova Ihor Kotsiumbas Hryhorii Tesliar |
| author_facet | Mariia Smolinska Roman Ostapiv Mariia Yurkevych Liubov Poliuzhyn Olha Korobova Ihor Kotsiumbas Hryhorii Tesliar |
| author_sort | Mariia Smolinska |
| collection | DOAJ |
| description | Simple, fast, and validated UV-spectrophotometric, HPLC, and GC methods for the analysis of benzalkonium chloride in a disinfectant were developed. UV-spectrophotometric determination was based on measuring the amount of light absorption of aqueous solutions of benzalkonium chloride at 268 nm. HPLC determination was achieved with a 150 mm × 4.6 mm, 5.0 μm C18 column. The mobile phase consisted of a 0.01% water solution of triethylamine (with pH 2.5) and acetonitrile in the ratio of 40 : 60 v/v. The column temperature was kept at 30°C, and the injection volume was 10 μL. The flow rate was 1.0 mL/min, and the diode array detector was set at 215 nm. GC determination was performed using a flame ionization detector on a glass capillary column ZB-WAX plus 30 m × 0.25 mm with an inner coating thickness of 0.25 μm. It creates a gradient increase in the temperature of the furnace to the maximum of 200°C. The temperature of the injector was 250°C, 1 μl was injected, and the separation of the sample was 1 : 100. Helium was used as a carrier gas, and the gas flow rate was constant, equaling 1.6 ml/min. The temperature of the detector was 250°C, and a mixture of hydrogen, helium, and air in a ratio of 30 : 8.5 : 350 was used in the split of the detector. All proposed methods were validated according to ICH guidelines with respect to the accuracy, precision (interday, intraday, and reproducibility), linearity, the limit of detection, the limit of quantitation, and robustness. All three methods were linear (R2 = 0.997–0.999) over a concentration range of 400–600 μg/ml for UV and 80–120 μg/ml for HPLC and GC, accurate (recovery was 98.4–101.7% for all methods), precise (RSD <2%), and robust. The cost of the analysis of one sample of the disinfectant for the content of benzalkonium chloride by three methods was calculated. The comparison of the obtained results facilitates a more efficient allocation of laboratory resources, depending on the goal. |
| format | Article |
| id | doaj-art-a953486b0082446f91a0f0714ec4d2c1 |
| institution | Kabale University |
| issn | 1687-8779 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Analytical Chemistry |
| spelling | doaj-art-a953486b0082446f91a0f0714ec4d2c12025-02-03T01:23:34ZengWileyInternational Journal of Analytical Chemistry1687-87792022-01-01202210.1155/2022/2932634Determination of Benzalkonium Chloride in a Disinfectant by UV Spectrophotometry and Gas and High-Performance Liquid Chromatography: Validation, Comparison of Characteristics, and Economic FeasibilityMariia Smolinska0Roman Ostapiv1Mariia Yurkevych2Liubov Poliuzhyn3Olha Korobova4Ihor Kotsiumbas5Hryhorii Tesliar6State Scientific Research Control Institute of Veterinary Preparations and Fodder AdditivesState Scientific Research Control Institute of Veterinary Preparations and Fodder AdditivesState Scientific Research Control Institute of Veterinary Preparations and Fodder AdditivesState Scientific Research Control Institute of Veterinary Preparations and Fodder AdditivesState Scientific Research Control Institute of Veterinary Preparations and Fodder AdditivesState Scientific Research Control Institute of Veterinary Preparations and Fodder AdditivesState Scientific Research Control Institute of Veterinary Preparations and Fodder AdditivesSimple, fast, and validated UV-spectrophotometric, HPLC, and GC methods for the analysis of benzalkonium chloride in a disinfectant were developed. UV-spectrophotometric determination was based on measuring the amount of light absorption of aqueous solutions of benzalkonium chloride at 268 nm. HPLC determination was achieved with a 150 mm × 4.6 mm, 5.0 μm C18 column. The mobile phase consisted of a 0.01% water solution of triethylamine (with pH 2.5) and acetonitrile in the ratio of 40 : 60 v/v. The column temperature was kept at 30°C, and the injection volume was 10 μL. The flow rate was 1.0 mL/min, and the diode array detector was set at 215 nm. GC determination was performed using a flame ionization detector on a glass capillary column ZB-WAX plus 30 m × 0.25 mm with an inner coating thickness of 0.25 μm. It creates a gradient increase in the temperature of the furnace to the maximum of 200°C. The temperature of the injector was 250°C, 1 μl was injected, and the separation of the sample was 1 : 100. Helium was used as a carrier gas, and the gas flow rate was constant, equaling 1.6 ml/min. The temperature of the detector was 250°C, and a mixture of hydrogen, helium, and air in a ratio of 30 : 8.5 : 350 was used in the split of the detector. All proposed methods were validated according to ICH guidelines with respect to the accuracy, precision (interday, intraday, and reproducibility), linearity, the limit of detection, the limit of quantitation, and robustness. All three methods were linear (R2 = 0.997–0.999) over a concentration range of 400–600 μg/ml for UV and 80–120 μg/ml for HPLC and GC, accurate (recovery was 98.4–101.7% for all methods), precise (RSD <2%), and robust. The cost of the analysis of one sample of the disinfectant for the content of benzalkonium chloride by three methods was calculated. The comparison of the obtained results facilitates a more efficient allocation of laboratory resources, depending on the goal.http://dx.doi.org/10.1155/2022/2932634 |
| spellingShingle | Mariia Smolinska Roman Ostapiv Mariia Yurkevych Liubov Poliuzhyn Olha Korobova Ihor Kotsiumbas Hryhorii Tesliar Determination of Benzalkonium Chloride in a Disinfectant by UV Spectrophotometry and Gas and High-Performance Liquid Chromatography: Validation, Comparison of Characteristics, and Economic Feasibility International Journal of Analytical Chemistry |
| title | Determination of Benzalkonium Chloride in a Disinfectant by UV Spectrophotometry and Gas and High-Performance Liquid Chromatography: Validation, Comparison of Characteristics, and Economic Feasibility |
| title_full | Determination of Benzalkonium Chloride in a Disinfectant by UV Spectrophotometry and Gas and High-Performance Liquid Chromatography: Validation, Comparison of Characteristics, and Economic Feasibility |
| title_fullStr | Determination of Benzalkonium Chloride in a Disinfectant by UV Spectrophotometry and Gas and High-Performance Liquid Chromatography: Validation, Comparison of Characteristics, and Economic Feasibility |
| title_full_unstemmed | Determination of Benzalkonium Chloride in a Disinfectant by UV Spectrophotometry and Gas and High-Performance Liquid Chromatography: Validation, Comparison of Characteristics, and Economic Feasibility |
| title_short | Determination of Benzalkonium Chloride in a Disinfectant by UV Spectrophotometry and Gas and High-Performance Liquid Chromatography: Validation, Comparison of Characteristics, and Economic Feasibility |
| title_sort | determination of benzalkonium chloride in a disinfectant by uv spectrophotometry and gas and high performance liquid chromatography validation comparison of characteristics and economic feasibility |
| url | http://dx.doi.org/10.1155/2022/2932634 |
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