Pulsed Electric Field Induces Significant Changes in the Metabolome of <i>Fusarium</i> Species and Decreases Their Viability and Toxigenicity
<i>Fusarium</i> fungi are widespread pathogens of food crops, primarily associated with the formation of mycotoxins. Therefore, effective mitigation strategies for these toxicogenic microorganisms are required. In this study, the potential of pulsed electric field (PEF) as an advanced te...
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2025-01-01
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| author | Adam Behner Jana Palicova Anna-Hirt Tobolkova Nela Prusova Milena Stranska |
| author_facet | Adam Behner Jana Palicova Anna-Hirt Tobolkova Nela Prusova Milena Stranska |
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| description | <i>Fusarium</i> fungi are widespread pathogens of food crops, primarily associated with the formation of mycotoxins. Therefore, effective mitigation strategies for these toxicogenic microorganisms are required. In this study, the potential of pulsed electric field (PEF) as an advanced technology of increasing use in the food processing industry was investigated to minimize the viability of <i>Fusarium</i> pathogens and to characterize the PEF-induced changes at the metabolomic level. Spores of four <i>Fusarium</i> species (<i>Fusarium culmorum</i>, <i>Fusarium graminearum</i>, <i>Fusarium poae</i>, and <i>Fusarium sporotrichioides</i>) were treated with PEF and cultured on potato dextrose agar (PDA) plates. The viability of the <i>Fusarium</i> species was assessed by counting the colony-forming units, and changes in the mycotoxin content and metabolomic fingerprints were evaluated by using UHPLC-HRMS/MS instrumental analysis. For metabolomic data processing and compound identification, the MS-DIAL (v. 4.80)–MS-CleanR–MS-Finder (v. 3.52) software platform was used. As we found out, both fungal viability and the ability to produce mycotoxins significantly decreased after the PEF treatment for all of the species tested. The metabolomes of the treated and untreated fungi showed statistically significant differences, and PEF-associated biomarkers from the classes oxidized fatty acid derivatives, cyclic hexapeptides, macrolides, pyranocoumarins, carbazoles, and guanidines were identified. |
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| spelling | doaj-art-a94ca703724c4adc975e2570826ef9a12025-01-24T13:51:16ZengMDPI AGToxins2072-66512025-01-011713310.3390/toxins17010033Pulsed Electric Field Induces Significant Changes in the Metabolome of <i>Fusarium</i> Species and Decreases Their Viability and ToxigenicityAdam Behner0Jana Palicova1Anna-Hirt Tobolkova2Nela Prusova3Milena Stranska4Department of Food Analysis and Nutrition, University of Chemistry and Technology, Prague, Technicka 3, 166 28 Prague, Czech RepublicCrop Research Institute in Prague, Drnovska 507/73, 161 00 Prague, Czech RepublicDepartment of Food Analysis and Nutrition, University of Chemistry and Technology, Prague, Technicka 3, 166 28 Prague, Czech RepublicDepartment of Food Analysis and Nutrition, University of Chemistry and Technology, Prague, Technicka 3, 166 28 Prague, Czech RepublicDepartment of Food Analysis and Nutrition, University of Chemistry and Technology, Prague, Technicka 3, 166 28 Prague, Czech Republic<i>Fusarium</i> fungi are widespread pathogens of food crops, primarily associated with the formation of mycotoxins. Therefore, effective mitigation strategies for these toxicogenic microorganisms are required. In this study, the potential of pulsed electric field (PEF) as an advanced technology of increasing use in the food processing industry was investigated to minimize the viability of <i>Fusarium</i> pathogens and to characterize the PEF-induced changes at the metabolomic level. Spores of four <i>Fusarium</i> species (<i>Fusarium culmorum</i>, <i>Fusarium graminearum</i>, <i>Fusarium poae</i>, and <i>Fusarium sporotrichioides</i>) were treated with PEF and cultured on potato dextrose agar (PDA) plates. The viability of the <i>Fusarium</i> species was assessed by counting the colony-forming units, and changes in the mycotoxin content and metabolomic fingerprints were evaluated by using UHPLC-HRMS/MS instrumental analysis. For metabolomic data processing and compound identification, the MS-DIAL (v. 4.80)–MS-CleanR–MS-Finder (v. 3.52) software platform was used. As we found out, both fungal viability and the ability to produce mycotoxins significantly decreased after the PEF treatment for all of the species tested. The metabolomes of the treated and untreated fungi showed statistically significant differences, and PEF-associated biomarkers from the classes oxidized fatty acid derivatives, cyclic hexapeptides, macrolides, pyranocoumarins, carbazoles, and guanidines were identified.https://www.mdpi.com/2072-6651/17/1/33<i>Fusarium</i>food pathogenspulsed electric fieldmetabolomic fingerprintingUHPLC-HRMS/MSbiomarkers |
| spellingShingle | Adam Behner Jana Palicova Anna-Hirt Tobolkova Nela Prusova Milena Stranska Pulsed Electric Field Induces Significant Changes in the Metabolome of <i>Fusarium</i> Species and Decreases Their Viability and Toxigenicity Toxins <i>Fusarium</i> food pathogens pulsed electric field metabolomic fingerprinting UHPLC-HRMS/MS biomarkers |
| title | Pulsed Electric Field Induces Significant Changes in the Metabolome of <i>Fusarium</i> Species and Decreases Their Viability and Toxigenicity |
| title_full | Pulsed Electric Field Induces Significant Changes in the Metabolome of <i>Fusarium</i> Species and Decreases Their Viability and Toxigenicity |
| title_fullStr | Pulsed Electric Field Induces Significant Changes in the Metabolome of <i>Fusarium</i> Species and Decreases Their Viability and Toxigenicity |
| title_full_unstemmed | Pulsed Electric Field Induces Significant Changes in the Metabolome of <i>Fusarium</i> Species and Decreases Their Viability and Toxigenicity |
| title_short | Pulsed Electric Field Induces Significant Changes in the Metabolome of <i>Fusarium</i> Species and Decreases Their Viability and Toxigenicity |
| title_sort | pulsed electric field induces significant changes in the metabolome of i fusarium i species and decreases their viability and toxigenicity |
| topic | <i>Fusarium</i> food pathogens pulsed electric field metabolomic fingerprinting UHPLC-HRMS/MS biomarkers |
| url | https://www.mdpi.com/2072-6651/17/1/33 |
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