The used of chopped banana Musa paradisiaca stem for stimulating immune responses and streptococcosis resistance of Nile tilapia Oreochromis niloticus
ABSTRACT Streptococcosis caused by Streptococcus agalactiae bacteria, is a type of disease that often found in Nile tilapia farming. This study was performed to determine the effectiveness of the concentration and frequency of giving chopped banana stem as the immunostimulant to stimulate the non...
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Asosiasi Sains Akuakultur Indonesia
2018-07-01
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| Series: | Jurnal Akuakultur Indonesia |
| Online Access: | https://journal.ipb.ac.id/index.php/jai/article/view/24213 |
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| author | Lilis Nurjanah Sri Nuryati Alimuddin Alimuddin Kukuh Nirmala |
| author_facet | Lilis Nurjanah Sri Nuryati Alimuddin Alimuddin Kukuh Nirmala |
| author_sort | Lilis Nurjanah |
| collection | DOAJ |
| description | ABSTRACT
Streptococcosis caused by Streptococcus agalactiae bacteria, is a type of disease that often found in Nile tilapia farming. This study was performed to determine the effectiveness of the concentration and frequency of giving chopped banana stem as the immunostimulant to stimulate the non‒specific immune system of tilapia against S. agalactiae infection. This study used factorial completely randomized design, consisted of two factors: concentration and changing frequency (replacement time interval) of banana stem on Nile tilapia rearing media with 11 treatment and each treatment was given three replications. The concentration of 5 g/L, 10 g/L and 15 g/L and the three days, seven days and no replacement time interval were used in this study. The immersion treatment with the chopped banana stem was done for 14 days, then the challenge test with S. agalactiae was on the 15th day for 14 days observation. The result showed that chopped banana stem contained active compounds of alkaloids, namely: 17.63% flavonoids, 0.02% tannin and 0.24% saponins. This active compounds could increase the non-specific immune system including respiratory burst, lysozyme activity, phagocyte activity, erythrocyte, leucocyte and haemoglobin. Treatment with a concentration of 5 g/L and no replacement of the chopped banana stem was the best treatment with 75% relative percent survival, highest than other treatments. In addition, it also has highest phagocytes (36.3%), respiratory burst (0.58 at O.D. 630), and lysozyme activity (72.7 unit/mL) after four days challenged with S. agalactiae.
Keywords: Banana stem, immunostimulant, Nile tilapia, non-specific immune system, Streptococcus agalatiae
ABSTRAK
Penyakit Streptococcosis yang disebabkan oleh bakteri Streptococcus agalactiae merupakan jenis penyakit yang sering ditemukan pada budidaya ikan nilaPenelitian ini bertujuan untuk menentukan konsentrasi dan frekuensi pergantian cacahan batang pisang sebagai imunostimulan yang optimum sehingga dapat meningkatkan daya tahan tubuh ikan nila terhadap serangan penyakit streptococcosis yang disebabkan oleh bakteri S. agalactiae. Penelitian ini menggunakan rancangan acak lengkap faktorial. Perlakuan terdiri dari dua faktor yaitu konsentrasi dan frekuensi pergantian cacahan batang pisang pada media pemeliharaan ikan dengan 11 perlakuan dan 3 ulangan. Konsentras batang pisang yang digunakan yaitu 5 g/L, 10 g/L dan 15 g/L, sedangkan frekuensi pergantian batang pisang dalam penelitian ini yaitu tiga hari, tujuh hari dan tanpa pergantian batang pisang. Perlakuan perendaman dengan cacahan batang pisang dilakukan selama 14 hari, kemudian dilakukan uji tantang dengan bakteri S. agalactiae pada hari ke-15 selama 14 hari pengamatan. Hasil penelitian menunjukkan bahwa cacahan batang pisang ambon mengandung senyawa aktif berupa alkaloid, flavonoid (17,63%), tanin (0,02%) dan saponin (0,24%). Senyawa aktif tersebut mampu meningkatkan respons imun non-spesifik pada ikan nila yaitu respiratory burst, aktivitas lisozim, aktivitas fagositik, total leukosit, total eritrosit dan hemoglobin. Perlakuan dengan konsentrasi 5 g/L dan tanpa pergantian cacahan batang pisang merupakan perlakuan terbaik dengan nilai relative percent survival (RPS) mencapai 70%, lebih besar dibandingkan dengan perlakuan lainnya. Selain itu, perlakuan ini memiliki nilai tertinggi pada aktivitas fagositik (36,6%), respiratory burst (0,58 pada O.D. 630 nm) dan aktivitas lisozim (72,7 Unit/mL) setelah hari ke‒4 uji tantang dengan bakteri S. agalactiae.
Kata kunci: batang pisang, ikan nila, imunostimulan, respons imun, Streptococcus agalactiae
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| format | Article |
| id | doaj-art-a945a71fd3124be2a008ad1d25a5ab60 |
| institution | Kabale University |
| issn | 1412-5269 2354-6700 |
| language | English |
| publishDate | 2018-07-01 |
| publisher | Asosiasi Sains Akuakultur Indonesia |
| record_format | Article |
| series | Jurnal Akuakultur Indonesia |
| spelling | doaj-art-a945a71fd3124be2a008ad1d25a5ab602025-01-24T17:57:24ZengAsosiasi Sains Akuakultur IndonesiaJurnal Akuakultur Indonesia1412-52692354-67002018-07-0117210.19027/jai.17.2.147-15715057The used of chopped banana Musa paradisiaca stem for stimulating immune responses and streptococcosis resistance of Nile tilapia Oreochromis niloticusLilis Nurjanah0Sri Nuryati1Alimuddin Alimuddin2Kukuh Nirmala3Department of Aquaculture, Faculty of Fisheries and Marine Sciences, Bogor Agricultural UniversityDepartment of Aquaculture, Faculty of Fisheries and Marine Sciences, Bogor Agricultural UniversityDepartment of Aquaculture, Faculty of Fisheries and Marine Sciences, Bogor Agricultural UniversityDepartment of Aquaculture, Faculty of Fisheries and Marine Sciences, Bogor Agricultural UniversityABSTRACT Streptococcosis caused by Streptococcus agalactiae bacteria, is a type of disease that often found in Nile tilapia farming. This study was performed to determine the effectiveness of the concentration and frequency of giving chopped banana stem as the immunostimulant to stimulate the non‒specific immune system of tilapia against S. agalactiae infection. This study used factorial completely randomized design, consisted of two factors: concentration and changing frequency (replacement time interval) of banana stem on Nile tilapia rearing media with 11 treatment and each treatment was given three replications. The concentration of 5 g/L, 10 g/L and 15 g/L and the three days, seven days and no replacement time interval were used in this study. The immersion treatment with the chopped banana stem was done for 14 days, then the challenge test with S. agalactiae was on the 15th day for 14 days observation. The result showed that chopped banana stem contained active compounds of alkaloids, namely: 17.63% flavonoids, 0.02% tannin and 0.24% saponins. This active compounds could increase the non-specific immune system including respiratory burst, lysozyme activity, phagocyte activity, erythrocyte, leucocyte and haemoglobin. Treatment with a concentration of 5 g/L and no replacement of the chopped banana stem was the best treatment with 75% relative percent survival, highest than other treatments. In addition, it also has highest phagocytes (36.3%), respiratory burst (0.58 at O.D. 630), and lysozyme activity (72.7 unit/mL) after four days challenged with S. agalactiae. Keywords: Banana stem, immunostimulant, Nile tilapia, non-specific immune system, Streptococcus agalatiae ABSTRAK Penyakit Streptococcosis yang disebabkan oleh bakteri Streptococcus agalactiae merupakan jenis penyakit yang sering ditemukan pada budidaya ikan nilaPenelitian ini bertujuan untuk menentukan konsentrasi dan frekuensi pergantian cacahan batang pisang sebagai imunostimulan yang optimum sehingga dapat meningkatkan daya tahan tubuh ikan nila terhadap serangan penyakit streptococcosis yang disebabkan oleh bakteri S. agalactiae. Penelitian ini menggunakan rancangan acak lengkap faktorial. Perlakuan terdiri dari dua faktor yaitu konsentrasi dan frekuensi pergantian cacahan batang pisang pada media pemeliharaan ikan dengan 11 perlakuan dan 3 ulangan. Konsentras batang pisang yang digunakan yaitu 5 g/L, 10 g/L dan 15 g/L, sedangkan frekuensi pergantian batang pisang dalam penelitian ini yaitu tiga hari, tujuh hari dan tanpa pergantian batang pisang. Perlakuan perendaman dengan cacahan batang pisang dilakukan selama 14 hari, kemudian dilakukan uji tantang dengan bakteri S. agalactiae pada hari ke-15 selama 14 hari pengamatan. Hasil penelitian menunjukkan bahwa cacahan batang pisang ambon mengandung senyawa aktif berupa alkaloid, flavonoid (17,63%), tanin (0,02%) dan saponin (0,24%). Senyawa aktif tersebut mampu meningkatkan respons imun non-spesifik pada ikan nila yaitu respiratory burst, aktivitas lisozim, aktivitas fagositik, total leukosit, total eritrosit dan hemoglobin. Perlakuan dengan konsentrasi 5 g/L dan tanpa pergantian cacahan batang pisang merupakan perlakuan terbaik dengan nilai relative percent survival (RPS) mencapai 70%, lebih besar dibandingkan dengan perlakuan lainnya. Selain itu, perlakuan ini memiliki nilai tertinggi pada aktivitas fagositik (36,6%), respiratory burst (0,58 pada O.D. 630 nm) dan aktivitas lisozim (72,7 Unit/mL) setelah hari ke‒4 uji tantang dengan bakteri S. agalactiae. Kata kunci: batang pisang, ikan nila, imunostimulan, respons imun, Streptococcus agalactiae https://journal.ipb.ac.id/index.php/jai/article/view/24213 |
| spellingShingle | Lilis Nurjanah Sri Nuryati Alimuddin Alimuddin Kukuh Nirmala The used of chopped banana Musa paradisiaca stem for stimulating immune responses and streptococcosis resistance of Nile tilapia Oreochromis niloticus Jurnal Akuakultur Indonesia |
| title | The used of chopped banana Musa paradisiaca stem for stimulating immune responses and streptococcosis resistance of Nile tilapia Oreochromis niloticus |
| title_full | The used of chopped banana Musa paradisiaca stem for stimulating immune responses and streptococcosis resistance of Nile tilapia Oreochromis niloticus |
| title_fullStr | The used of chopped banana Musa paradisiaca stem for stimulating immune responses and streptococcosis resistance of Nile tilapia Oreochromis niloticus |
| title_full_unstemmed | The used of chopped banana Musa paradisiaca stem for stimulating immune responses and streptococcosis resistance of Nile tilapia Oreochromis niloticus |
| title_short | The used of chopped banana Musa paradisiaca stem for stimulating immune responses and streptococcosis resistance of Nile tilapia Oreochromis niloticus |
| title_sort | used of chopped banana musa paradisiaca stem for stimulating immune responses and streptococcosis resistance of nile tilapia oreochromis niloticus |
| url | https://journal.ipb.ac.id/index.php/jai/article/view/24213 |
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