Robust intrapulmonary CD8 T cell responses and protection with an attenuated N1L deleted vaccinia virus.
<h4>Background</h4>Vaccinia viruses have been used as a model for viral disease and as a protective live vaccine.<h4>Methodology and principal findings</h4>We investigated the immunogenicity of an attenuated strain of vaccinia virus engineered to inactivate the N1L gene (vGK5...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2008-10-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0003323&type=printable |
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| Summary: | <h4>Background</h4>Vaccinia viruses have been used as a model for viral disease and as a protective live vaccine.<h4>Methodology and principal findings</h4>We investigated the immunogenicity of an attenuated strain of vaccinia virus engineered to inactivate the N1L gene (vGK5). Using the intranasal route, this recombinant virus was 2 logs less virulent compared to the wildtype VACV-WR. Infection by the intranasal, intraperitoneal, and tail scarification routes resulted in the robust induction of cytolytic virus-specific CD8 T cells in the spleens and the lungs. VACV-specific antibodies were also detected in the sera of mice infected 3-5 months prior with the attenuated vGK5 virus. Finally, mice immunized with vGK5 were significantly protected when challenged with a lethal dose of VACV-WR.<h4>Conclusions</h4>These results indicate that the attenuated vGK5 virus protects against subsequent infection and suggest that the N1L protein limits the strength of the early antiviral CD8 T cell response following respiratory infection. |
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| ISSN: | 1932-6203 |