RNASeq analysis of drought-stressed guayule reveals the role of gene transcription for modulating rubber, resin, and carbohydrate synthesis
Abstract The drought-adapted shrub guayule (Parthenium argentatum) produces rubber, a natural product of major commercial importance, and two co-products with potential industrial use: terpene resin and the carbohydrate fructan. The rubber content of guayule plants subjected to water stress is highe...
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| Format: | Article |
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Nature Portfolio
2021-11-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-01026-7 |
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| author | Chen Dong Grisel Ponciano Naxin Huo Yong Gu Daniel Ilut Colleen McMahan |
| author_facet | Chen Dong Grisel Ponciano Naxin Huo Yong Gu Daniel Ilut Colleen McMahan |
| author_sort | Chen Dong |
| collection | DOAJ |
| description | Abstract The drought-adapted shrub guayule (Parthenium argentatum) produces rubber, a natural product of major commercial importance, and two co-products with potential industrial use: terpene resin and the carbohydrate fructan. The rubber content of guayule plants subjected to water stress is higher compared to that of well-irrigated plants, a fact consistently reported in guayule field evaluations. To better understand how drought influences rubber biosynthesis at the molecular level, a comprehensive transcriptome database was built from drought-stressed guayule stem tissues using de novo RNA-seq and genome-guided assembly, followed by annotation and expression analysis. Despite having higher rubber content, most rubber biosynthesis related genes were down-regulated in drought-stressed guayule, compared to well-irrigated plants, suggesting post-transcriptional effects may regulate drought-induced rubber accumulation. On the other hand, terpene resin biosynthesis genes were unevenly affected by water stress, implying unique environmental influences over transcriptional control of different terpene compounds or classes. Finally, drought induced expression of fructan catabolism genes in guayule and significantly suppressed these fructan biosynthesis genes. It appears then, that in guayule cultivation, irrigation levels might be calibrated in such a regime to enable tunable accumulation of rubber, resin and fructan. |
| format | Article |
| id | doaj-art-a9281b284b224244b7d371f795b2222a |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2021-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-a9281b284b224244b7d371f795b2222a2025-01-19T12:25:05ZengNature PortfolioScientific Reports2045-23222021-11-0111111610.1038/s41598-021-01026-7RNASeq analysis of drought-stressed guayule reveals the role of gene transcription for modulating rubber, resin, and carbohydrate synthesisChen Dong0Grisel Ponciano1Naxin Huo2Yong Gu3Daniel Ilut4Colleen McMahan5USDA Agricultural Research Service, Western Regional Research CenterUSDA Agricultural Research Service, Western Regional Research CenterUSDA Agricultural Research Service, Western Regional Research CenterUSDA Agricultural Research Service, Western Regional Research CenterPlant Breeding and Genetics Section, School of Integrative Plant Science, Cornell UniversityUSDA Agricultural Research Service, Western Regional Research CenterAbstract The drought-adapted shrub guayule (Parthenium argentatum) produces rubber, a natural product of major commercial importance, and two co-products with potential industrial use: terpene resin and the carbohydrate fructan. The rubber content of guayule plants subjected to water stress is higher compared to that of well-irrigated plants, a fact consistently reported in guayule field evaluations. To better understand how drought influences rubber biosynthesis at the molecular level, a comprehensive transcriptome database was built from drought-stressed guayule stem tissues using de novo RNA-seq and genome-guided assembly, followed by annotation and expression analysis. Despite having higher rubber content, most rubber biosynthesis related genes were down-regulated in drought-stressed guayule, compared to well-irrigated plants, suggesting post-transcriptional effects may regulate drought-induced rubber accumulation. On the other hand, terpene resin biosynthesis genes were unevenly affected by water stress, implying unique environmental influences over transcriptional control of different terpene compounds or classes. Finally, drought induced expression of fructan catabolism genes in guayule and significantly suppressed these fructan biosynthesis genes. It appears then, that in guayule cultivation, irrigation levels might be calibrated in such a regime to enable tunable accumulation of rubber, resin and fructan.https://doi.org/10.1038/s41598-021-01026-7 |
| spellingShingle | Chen Dong Grisel Ponciano Naxin Huo Yong Gu Daniel Ilut Colleen McMahan RNASeq analysis of drought-stressed guayule reveals the role of gene transcription for modulating rubber, resin, and carbohydrate synthesis Scientific Reports |
| title | RNASeq analysis of drought-stressed guayule reveals the role of gene transcription for modulating rubber, resin, and carbohydrate synthesis |
| title_full | RNASeq analysis of drought-stressed guayule reveals the role of gene transcription for modulating rubber, resin, and carbohydrate synthesis |
| title_fullStr | RNASeq analysis of drought-stressed guayule reveals the role of gene transcription for modulating rubber, resin, and carbohydrate synthesis |
| title_full_unstemmed | RNASeq analysis of drought-stressed guayule reveals the role of gene transcription for modulating rubber, resin, and carbohydrate synthesis |
| title_short | RNASeq analysis of drought-stressed guayule reveals the role of gene transcription for modulating rubber, resin, and carbohydrate synthesis |
| title_sort | rnaseq analysis of drought stressed guayule reveals the role of gene transcription for modulating rubber resin and carbohydrate synthesis |
| url | https://doi.org/10.1038/s41598-021-01026-7 |
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