Phenotyping of Leukocytes and Leukocyte-Derived Extracellular Vesicles
Extracellular vesicles (EVs) have a demonstrated involvement in modulating the immune system. It has been proposed that EVs could be used as biomarkers for detection of inflammatory and immunological disorders. Consequently, it is of great interest to investigate EVs in more detail with focus on imm...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2016/6391264 |
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| _version_ | 1832561517362413568 |
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| author | Lotte Hatting Pugholm Rikke Bæk Evo Kristina Lindersson Søndergaard Anne Louise Schacht Revenfeld Malene Møller Jørgensen Kim Varming |
| author_facet | Lotte Hatting Pugholm Rikke Bæk Evo Kristina Lindersson Søndergaard Anne Louise Schacht Revenfeld Malene Møller Jørgensen Kim Varming |
| author_sort | Lotte Hatting Pugholm |
| collection | DOAJ |
| description | Extracellular vesicles (EVs) have a demonstrated involvement in modulating the immune system. It has been proposed that EVs could be used as biomarkers for detection of inflammatory and immunological disorders. Consequently, it is of great interest to investigate EVs in more detail with focus on immunological markers. In this study, five major leukocyte subpopulations and the corresponding leukocyte-derived EVs were phenotyped with focus on selected immunological lineage-specific markers and selected vesicle-related markers. The leukocyte-derived EVs displayed phenotypic differences in the 34 markers investigated. The majority of the lineage-specific markers used for identification of the parent cell types could not be detected on EVs released from monocultures of the associated cell types. In contrast, the vesicular presentation of CD9, CD63, and CD81 correlated to the cell surface expression of these markers, however, with few exceptions. Furthermore, the cellular expression of CD9, CD63, and CD81 varied between leukocytes present in whole blood and cultured leukocytes. In summary, these data demonstrate that the cellular and vesicular presentation of selected lineage-specific and vesicle-related markers may differ, supporting the accumulating observations that sorting of molecular cargo into EVs is tightly controlled. |
| format | Article |
| id | doaj-art-a90945540a0b49b59ede316b03405e10 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-a90945540a0b49b59ede316b03405e102025-02-03T01:24:55ZengWileyJournal of Immunology Research2314-88612314-71562016-01-01201610.1155/2016/63912646391264Phenotyping of Leukocytes and Leukocyte-Derived Extracellular VesiclesLotte Hatting Pugholm0Rikke Bæk1Evo Kristina Lindersson Søndergaard2Anne Louise Schacht Revenfeld3Malene Møller Jørgensen4Kim Varming5Department of Clinical Immunology, Aalborg University Hospital, 9000 Aalborg, DenmarkDepartment of Clinical Immunology, Aalborg University Hospital, 9000 Aalborg, DenmarkDepartment of Clinical Immunology, Aalborg University Hospital, 9000 Aalborg, DenmarkDepartment of Clinical Immunology, Aalborg University Hospital, 9000 Aalborg, DenmarkDepartment of Clinical Immunology, Aalborg University Hospital, 9000 Aalborg, DenmarkDepartment of Clinical Immunology, Aalborg University Hospital, 9000 Aalborg, DenmarkExtracellular vesicles (EVs) have a demonstrated involvement in modulating the immune system. It has been proposed that EVs could be used as biomarkers for detection of inflammatory and immunological disorders. Consequently, it is of great interest to investigate EVs in more detail with focus on immunological markers. In this study, five major leukocyte subpopulations and the corresponding leukocyte-derived EVs were phenotyped with focus on selected immunological lineage-specific markers and selected vesicle-related markers. The leukocyte-derived EVs displayed phenotypic differences in the 34 markers investigated. The majority of the lineage-specific markers used for identification of the parent cell types could not be detected on EVs released from monocultures of the associated cell types. In contrast, the vesicular presentation of CD9, CD63, and CD81 correlated to the cell surface expression of these markers, however, with few exceptions. Furthermore, the cellular expression of CD9, CD63, and CD81 varied between leukocytes present in whole blood and cultured leukocytes. In summary, these data demonstrate that the cellular and vesicular presentation of selected lineage-specific and vesicle-related markers may differ, supporting the accumulating observations that sorting of molecular cargo into EVs is tightly controlled.http://dx.doi.org/10.1155/2016/6391264 |
| spellingShingle | Lotte Hatting Pugholm Rikke Bæk Evo Kristina Lindersson Søndergaard Anne Louise Schacht Revenfeld Malene Møller Jørgensen Kim Varming Phenotyping of Leukocytes and Leukocyte-Derived Extracellular Vesicles Journal of Immunology Research |
| title | Phenotyping of Leukocytes and Leukocyte-Derived Extracellular Vesicles |
| title_full | Phenotyping of Leukocytes and Leukocyte-Derived Extracellular Vesicles |
| title_fullStr | Phenotyping of Leukocytes and Leukocyte-Derived Extracellular Vesicles |
| title_full_unstemmed | Phenotyping of Leukocytes and Leukocyte-Derived Extracellular Vesicles |
| title_short | Phenotyping of Leukocytes and Leukocyte-Derived Extracellular Vesicles |
| title_sort | phenotyping of leukocytes and leukocyte derived extracellular vesicles |
| url | http://dx.doi.org/10.1155/2016/6391264 |
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