Growth Pattern and Copper Accumulation in Callus of Datura metel
<p>This experiment was aimed to evaluate the copper accumulation using callus culture of <em>Datura metel</em> L. The culture was established from leaves onto MS contained NAA 2.5 mg/L and Kinetin 0.5 mg/L as the control. The exposure of the culture was carried out by 2 copper comp...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Universitas Negeri Semarang
2016-09-01
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| Series: | Biosaintifika: Journal of Biology & Biology Education |
| Subjects: | |
| Online Access: | https://journal.unnes.ac.id/nju/index.php/biosaintifika/article/view/5177 |
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| Summary: | <p>This experiment was aimed to evaluate the copper accumulation using callus culture of <em>Datura metel</em> L. The culture was established from leaves onto MS contained NAA 2.5 mg/L and Kinetin 0.5 mg/L as the control. The exposure of the culture was carried out by 2 copper compounds as treatment, i.e. CuCl<sub>2</sub>.2H<sub>2</sub>O and Na<sub>2</sub>CuEDTA at level concentration 0.; 0.1; 5; 10; 15; and 20 µM. The growth pattern of callus in control showed increasing growth rate in 36 days, whereas exponential stage was reached at 12-20<sup>th</sup> doi*. Whilst, after 10 doi, the treatment showed constant growth pattern. The absorption rate of the culture was increased by the addition of the CuCl<sub>2</sub>.2H<sub>2</sub>O at 5 – 15 µM of level concentration but declined at 20µM. The maximum rate of accumulation of Cu (0,1519 mg g<sup>-1</sup>) was obtained at 15 µM. Instead, the addition of Na<sub>2</sub>CuEDTA at 5 – 20µM of level concentration showed the significant increment while the maximum accumulation was obtained at 20µM (0,1420 mg g<sup>-1</sup>). The existence of chelator in copper compound reduced the rate of toxicity while all tolerance index values were between 66,24 and 97,28 %.The results suggested the role of callus of <em>D. metel</em> as that fairly absorbed and accumulated Cu<sup>2+</sup>. Exposure with CuCl<sub>2</sub>.2H<sub>2</sub>O indicated higher accumulation than Na<sub>2</sub>CuEDTA.</p><p><strong>How to Cite</strong></p><p>Nurchayati, Y., Santosa, S., Nugroho, L. H., & Indrianto, A. (2016). Growth Pattern and Copper Accumulation in Callus of Datura metel. <em>Biosaintifika: Journal of Biology & Biology Education</em>, 8(2), 135-140. </p> |
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| ISSN: | 2085-191X 2338-7610 |