The big chill: Growth of in situ structural biology with cryo-electron tomography
In situ structural biology with cryo-electron tomography (cryo-ET) and subtomogram averaging (StA) is evolving as a major method to understand the structure, function, and interactions of biological molecules in cells in a single experiment. Since its inception, the method has matured with some stel...
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| Format: | Article |
| Language: | English |
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Cambridge University Press
2024-01-01
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| Series: | QRB Discovery |
| Online Access: | https://www.cambridge.org/core/product/identifier/S2633289224000103/type/journal_article |
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| author | Mikhail Kudryashev |
| author_facet | Mikhail Kudryashev |
| author_sort | Mikhail Kudryashev |
| collection | DOAJ |
| description | In situ structural biology with cryo-electron tomography (cryo-ET) and subtomogram averaging (StA) is evolving as a major method to understand the structure, function, and interactions of biological molecules in cells in a single experiment. Since its inception, the method has matured with some stellar highlights and with further opportunities to broaden its applications. In this short review, I want to provide a personal perspective on the developments in cryo-ET as I have seen it for the last ~20 years and outline the major steps that led to its success. This perspective highlights cryo-ET with my eyes as a junior researcher and my view on the present and past developments in hardware and software for in situ structural biology with cryo-ET. |
| format | Article |
| id | doaj-art-a8db3076433b47358b1c0c9f90c03579 |
| institution | Kabale University |
| issn | 2633-2892 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Cambridge University Press |
| record_format | Article |
| series | QRB Discovery |
| spelling | doaj-art-a8db3076433b47358b1c0c9f90c035792025-02-04T03:42:22ZengCambridge University PressQRB Discovery2633-28922024-01-01510.1017/qrd.2024.10The big chill: Growth of in situ structural biology with cryo-electron tomographyMikhail Kudryashev0https://orcid.org/0000-0003-3550-6274In situ Structural Biology, Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany Institute of Medical Physics and Biophysics, Charite–Universitatsmedizin Berlin, corporate member of Freie Universitat Berlin and Humboldt Universitat zu Berlin, Institute for Medical Physics and Biophysics, Berlin, GermanyIn situ structural biology with cryo-electron tomography (cryo-ET) and subtomogram averaging (StA) is evolving as a major method to understand the structure, function, and interactions of biological molecules in cells in a single experiment. Since its inception, the method has matured with some stellar highlights and with further opportunities to broaden its applications. In this short review, I want to provide a personal perspective on the developments in cryo-ET as I have seen it for the last ~20 years and outline the major steps that led to its success. This perspective highlights cryo-ET with my eyes as a junior researcher and my view on the present and past developments in hardware and software for in situ structural biology with cryo-ET.https://www.cambridge.org/core/product/identifier/S2633289224000103/type/journal_article |
| spellingShingle | Mikhail Kudryashev The big chill: Growth of in situ structural biology with cryo-electron tomography QRB Discovery |
| title | The big chill: Growth of in situ structural biology with cryo-electron tomography |
| title_full | The big chill: Growth of in situ structural biology with cryo-electron tomography |
| title_fullStr | The big chill: Growth of in situ structural biology with cryo-electron tomography |
| title_full_unstemmed | The big chill: Growth of in situ structural biology with cryo-electron tomography |
| title_short | The big chill: Growth of in situ structural biology with cryo-electron tomography |
| title_sort | big chill growth of in situ structural biology with cryo electron tomography |
| url | https://www.cambridge.org/core/product/identifier/S2633289224000103/type/journal_article |
| work_keys_str_mv | AT mikhailkudryashev thebigchillgrowthofinsitustructuralbiologywithcryoelectrontomography AT mikhailkudryashev bigchillgrowthofinsitustructuralbiologywithcryoelectrontomography |