The big chill: Growth of in situ structural biology with cryo-electron tomography
In situ structural biology with cryo-electron tomography (cryo-ET) and subtomogram averaging (StA) is evolving as a major method to understand the structure, function, and interactions of biological molecules in cells in a single experiment. Since its inception, the method has matured with some stel...
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| Format: | Article |
| Language: | English |
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Cambridge University Press
2024-01-01
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| Series: | QRB Discovery |
| Online Access: | https://www.cambridge.org/core/product/identifier/S2633289224000103/type/journal_article |
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| Summary: | In situ structural biology with cryo-electron tomography (cryo-ET) and subtomogram averaging (StA) is evolving as a major method to understand the structure, function, and interactions of biological molecules in cells in a single experiment. Since its inception, the method has matured with some stellar highlights and with further opportunities to broaden its applications. In this short review, I want to provide a personal perspective on the developments in cryo-ET as I have seen it for the last ~20 years and outline the major steps that led to its success. This perspective highlights cryo-ET with my eyes as a junior researcher and my view on the present and past developments in hardware and software for in situ structural biology with cryo-ET. |
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| ISSN: | 2633-2892 |