Comparative analysis of acids and temperatures in gelatin extraction from the skin of yellowfin tuna (Thunnus albacares)
Gelatin, a valuable biopolymer, is commonly derived from animal by-products such as fish skin, providing a sustainable alternative to traditional bovine and porcine sources. The extraction process is influenced by factors like acid type and temperature, which impact both yield and quality. This stud...
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Universitas Syiah Kuala
2024-12-01
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| Series: | Depik Jurnal |
| Online Access: | https://jurnal.usk.ac.id/depik/article/view/41269 |
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| author | Vicky Prajaputra Sri Agustina Nadia Isnaini Agillia Nabila Hikma Kasih Margolang Sahra Apriani Siti Maqfirah |
| author_facet | Vicky Prajaputra Sri Agustina Nadia Isnaini Agillia Nabila Hikma Kasih Margolang Sahra Apriani Siti Maqfirah |
| author_sort | Vicky Prajaputra |
| collection | DOAJ |
| description | Gelatin, a valuable biopolymer, is commonly derived from animal by-products such as fish skin, providing a sustainable alternative to traditional bovine and porcine sources. The extraction process is influenced by factors like acid type and temperature, which impact both yield and quality. This study aimed to compare the effects of citric acid and acetic acid, at two temperatures (50°C and 60°C), on the gelatin extracted from yellowfin tuna (Thunnus albacares) skin. The extracted gelatin was evaluated for yield, proximate composition, functional groups using FTIR, and surface morphology through SEM. Results indicated that acetic acid produced a significantly higher yield than citric acid at both temperatures, with the highest yield (10.10%) at 50°C and the lowest (2.46%) from citric acid at 60°C. Gelatin extracted with acetic acid also had higher protein content and lower moisture and ash levels. FTIR analysis confirmed the presence of characteristic gelatin functional groups, including Amide I, II, and III. SEM images showed a denser and more uniform structure in acetic acid-extracted gelatin, while gelatin extracted with citric acid had a more porous and irregular surface. SDS-PAGE analysis revealed stronger protein bands for gelatin extracted with acetic acid, indicating higher protein content and a more efficient extraction process. Both acid-extracted gelatins showed α-chains and β-chains, characteristic of denatured collagen. In conclusion, acetic acid proved to be more effective than citric acid in producing higher yield and superior quality gelatin from yellowfin tuna skin. Further studies should assess the gel strength and amino acid composition of the extracted gelatin.
Keywords:
environmental chemistry
below water
biopolymer
gelatin
marine chemistry
waste valorization
yellowfin tuna |
| format | Article |
| id | doaj-art-a8ae9b58140a41dea935203bebb99aea |
| institution | Kabale University |
| issn | 2089-7790 2502-6194 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Universitas Syiah Kuala |
| record_format | Article |
| series | Depik Jurnal |
| spelling | doaj-art-a8ae9b58140a41dea935203bebb99aea2025-01-21T06:00:42ZengUniversitas Syiah KualaDepik Jurnal2089-77902502-61942024-12-0113346947510.13170/depik.13.3.4126919521Comparative analysis of acids and temperatures in gelatin extraction from the skin of yellowfin tuna (Thunnus albacares)Vicky Prajaputra0Sri Agustina1Nadia Isnaini2Agillia Nabila3Hikma Kasih Margolang4Sahra Apriani5Siti Maqfirah6Department of Marine Sciences, Faculty of Fisheries and Marine Sciences, Universitas Syiah KualaDepartment of Marine Sciences, Faculty of Fisheries and Marine Sciences, Universitas Syiah KualaDepartment of Pharmacy, Faculty of Mathematics and Natural Sciences, Universitas Syiah KualaDepartment of Marine Sciences, Faculty of Fisheries and Marine Sciences, Universitas Syiah KualaDepartment of Marine Sciences, Faculty of Fisheries and Marine Sciences, Universitas Syiah KualaDepartment of Marine Sciences, Faculty of Fisheries and Marine Sciences, Universitas Syiah KualaDepartment of Marine Sciences, Faculty of Fisheries and Marine Sciences, Universitas Syiah KualaGelatin, a valuable biopolymer, is commonly derived from animal by-products such as fish skin, providing a sustainable alternative to traditional bovine and porcine sources. The extraction process is influenced by factors like acid type and temperature, which impact both yield and quality. This study aimed to compare the effects of citric acid and acetic acid, at two temperatures (50°C and 60°C), on the gelatin extracted from yellowfin tuna (Thunnus albacares) skin. The extracted gelatin was evaluated for yield, proximate composition, functional groups using FTIR, and surface morphology through SEM. Results indicated that acetic acid produced a significantly higher yield than citric acid at both temperatures, with the highest yield (10.10%) at 50°C and the lowest (2.46%) from citric acid at 60°C. Gelatin extracted with acetic acid also had higher protein content and lower moisture and ash levels. FTIR analysis confirmed the presence of characteristic gelatin functional groups, including Amide I, II, and III. SEM images showed a denser and more uniform structure in acetic acid-extracted gelatin, while gelatin extracted with citric acid had a more porous and irregular surface. SDS-PAGE analysis revealed stronger protein bands for gelatin extracted with acetic acid, indicating higher protein content and a more efficient extraction process. Both acid-extracted gelatins showed α-chains and β-chains, characteristic of denatured collagen. In conclusion, acetic acid proved to be more effective than citric acid in producing higher yield and superior quality gelatin from yellowfin tuna skin. Further studies should assess the gel strength and amino acid composition of the extracted gelatin. Keywords: environmental chemistry below water biopolymer gelatin marine chemistry waste valorization yellowfin tunahttps://jurnal.usk.ac.id/depik/article/view/41269 |
| spellingShingle | Vicky Prajaputra Sri Agustina Nadia Isnaini Agillia Nabila Hikma Kasih Margolang Sahra Apriani Siti Maqfirah Comparative analysis of acids and temperatures in gelatin extraction from the skin of yellowfin tuna (Thunnus albacares) Depik Jurnal |
| title | Comparative analysis of acids and temperatures in gelatin extraction from the skin of yellowfin tuna (Thunnus albacares) |
| title_full | Comparative analysis of acids and temperatures in gelatin extraction from the skin of yellowfin tuna (Thunnus albacares) |
| title_fullStr | Comparative analysis of acids and temperatures in gelatin extraction from the skin of yellowfin tuna (Thunnus albacares) |
| title_full_unstemmed | Comparative analysis of acids and temperatures in gelatin extraction from the skin of yellowfin tuna (Thunnus albacares) |
| title_short | Comparative analysis of acids and temperatures in gelatin extraction from the skin of yellowfin tuna (Thunnus albacares) |
| title_sort | comparative analysis of acids and temperatures in gelatin extraction from the skin of yellowfin tuna thunnus albacares |
| url | https://jurnal.usk.ac.id/depik/article/view/41269 |
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