Thermal Study of a Newly Synthesized Cu(II) Complex Binding to Bovine β-Lactoglobulin
We have investigated the interactions between β-lactoglobulin, BLG, and new synthesized Cu(II) complex (2,2′-dibipyridine Cu(II) chloride) using isothermal titration calorimetry (ITC) methods at different temperatures of 298 and 310 K. The heats of BLG + Cu(II) interactions are reported and analyzed...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2013/453056 |
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| author | Adeleh Divsalar Lyla Barzegar Gholamreza Rezaei Behbehani |
| author_facet | Adeleh Divsalar Lyla Barzegar Gholamreza Rezaei Behbehani |
| author_sort | Adeleh Divsalar |
| collection | DOAJ |
| description | We have investigated the interactions between β-lactoglobulin, BLG, and new synthesized Cu(II) complex (2,2′-dibipyridine Cu(II) chloride) using isothermal titration calorimetry (ITC) methods at different temperatures of 298 and 310 K. The heats of BLG + Cu(II) interactions are reported and analyzed in terms of the extended solvation theory for calculation of binding and thermodynamic parameters of the interaction. The results suggested that binding of Cu(II) complex on BLG resulted in significant changes on the tertiary structure and conformation of protein via increasing of hydrophobicity and inducing partially unfolded structure in BLG which has a good agreement with the solvation parameters recovered by the extended solvation model suggesting destabilization of the protein. |
| format | Article |
| id | doaj-art-a89b5032897442ffbdab27196ff16d11 |
| institution | Kabale University |
| issn | 2090-9063 2090-9071 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Chemistry |
| spelling | doaj-art-a89b5032897442ffbdab27196ff16d112025-02-03T05:58:58ZengWileyJournal of Chemistry2090-90632090-90712013-01-01201310.1155/2013/453056453056Thermal Study of a Newly Synthesized Cu(II) Complex Binding to Bovine β-LactoglobulinAdeleh Divsalar0Lyla Barzegar1Gholamreza Rezaei Behbehani2Institute of Biochemistry and Biophysics, University of Tehran, Tehran, IranChemistry Department, Faculty of Science, Islamic Azad University, Takestan Branch, Takestan, IranChemistry Department, Imam Khomeini International University, Qazvin, IranWe have investigated the interactions between β-lactoglobulin, BLG, and new synthesized Cu(II) complex (2,2′-dibipyridine Cu(II) chloride) using isothermal titration calorimetry (ITC) methods at different temperatures of 298 and 310 K. The heats of BLG + Cu(II) interactions are reported and analyzed in terms of the extended solvation theory for calculation of binding and thermodynamic parameters of the interaction. The results suggested that binding of Cu(II) complex on BLG resulted in significant changes on the tertiary structure and conformation of protein via increasing of hydrophobicity and inducing partially unfolded structure in BLG which has a good agreement with the solvation parameters recovered by the extended solvation model suggesting destabilization of the protein.http://dx.doi.org/10.1155/2013/453056 |
| spellingShingle | Adeleh Divsalar Lyla Barzegar Gholamreza Rezaei Behbehani Thermal Study of a Newly Synthesized Cu(II) Complex Binding to Bovine β-Lactoglobulin Journal of Chemistry |
| title | Thermal Study of a Newly Synthesized Cu(II) Complex Binding to Bovine β-Lactoglobulin |
| title_full | Thermal Study of a Newly Synthesized Cu(II) Complex Binding to Bovine β-Lactoglobulin |
| title_fullStr | Thermal Study of a Newly Synthesized Cu(II) Complex Binding to Bovine β-Lactoglobulin |
| title_full_unstemmed | Thermal Study of a Newly Synthesized Cu(II) Complex Binding to Bovine β-Lactoglobulin |
| title_short | Thermal Study of a Newly Synthesized Cu(II) Complex Binding to Bovine β-Lactoglobulin |
| title_sort | thermal study of a newly synthesized cu ii complex binding to bovine β lactoglobulin |
| url | http://dx.doi.org/10.1155/2013/453056 |
| work_keys_str_mv | AT adelehdivsalar thermalstudyofanewlysynthesizedcuiicomplexbindingtobovineblactoglobulin AT lylabarzegar thermalstudyofanewlysynthesizedcuiicomplexbindingtobovineblactoglobulin AT gholamrezarezaeibehbehani thermalstudyofanewlysynthesizedcuiicomplexbindingtobovineblactoglobulin |