Non‐destructive, whole‐plant phenotyping reveals dynamic changes in water use efficiency, photosynthesis, and rhizosphere acidification of sorghum accessions under osmotic stress
Abstract Noninvasive phenotyping can quantify dynamic plant growth processes at higher temporal resolution than destructive phenotyping and can reveal phenomena that would be missed by end‐point analysis alone. Additionally, whole‐plant phenotyping can identify growth conditions that are optimal for...
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| Format: | Article |
| Language: | English |
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Wiley
2024-03-01
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| Series: | Plant Direct |
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| Online Access: | https://doi.org/10.1002/pld3.571 |
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| author | Daniel N. Ginzburg Jack A. Cox Seung Y. Rhee |
| author_facet | Daniel N. Ginzburg Jack A. Cox Seung Y. Rhee |
| author_sort | Daniel N. Ginzburg |
| collection | DOAJ |
| description | Abstract Noninvasive phenotyping can quantify dynamic plant growth processes at higher temporal resolution than destructive phenotyping and can reveal phenomena that would be missed by end‐point analysis alone. Additionally, whole‐plant phenotyping can identify growth conditions that are optimal for both above‐ and below‐ground tissues. However, noninvasive, whole‐plant phenotyping approaches available today are generally expensive, complex, and non‐modular. We developed a low‐cost and versatile approach to noninvasively measure whole‐plant physiology over time by growing plants in isolated hydroponic chambers. We demonstrate the versatility of our approach by measuring whole‐plant biomass accumulation, water use, and water use efficiency every two days on unstressed and osmotically stressed sorghum accessions. We identified relationships between root zone acidification and photosynthesis on whole‐plant water use efficiency over time. Our system can be implemented using cheap, basic components, requires no specific technical expertise, and should be suitable for any non‐aquatic vascular plant species. |
| format | Article |
| id | doaj-art-a86e52cbbaf24ab49ff66dc1c603f624 |
| institution | Kabale University |
| issn | 2475-4455 |
| language | English |
| publishDate | 2024-03-01 |
| publisher | Wiley |
| record_format | Article |
| series | Plant Direct |
| spelling | doaj-art-a86e52cbbaf24ab49ff66dc1c603f6242025-02-04T08:31:05ZengWileyPlant Direct2475-44552024-03-0183n/an/a10.1002/pld3.571Non‐destructive, whole‐plant phenotyping reveals dynamic changes in water use efficiency, photosynthesis, and rhizosphere acidification of sorghum accessions under osmotic stressDaniel N. Ginzburg0Jack A. Cox1Seung Y. Rhee2Department of Plant Biology Carnegie Institution for Science Stanford California USADepartment of Plant Biology Carnegie Institution for Science Stanford California USADepartment of Plant Biology Carnegie Institution for Science Stanford California USAAbstract Noninvasive phenotyping can quantify dynamic plant growth processes at higher temporal resolution than destructive phenotyping and can reveal phenomena that would be missed by end‐point analysis alone. Additionally, whole‐plant phenotyping can identify growth conditions that are optimal for both above‐ and below‐ground tissues. However, noninvasive, whole‐plant phenotyping approaches available today are generally expensive, complex, and non‐modular. We developed a low‐cost and versatile approach to noninvasively measure whole‐plant physiology over time by growing plants in isolated hydroponic chambers. We demonstrate the versatility of our approach by measuring whole‐plant biomass accumulation, water use, and water use efficiency every two days on unstressed and osmotically stressed sorghum accessions. We identified relationships between root zone acidification and photosynthesis on whole‐plant water use efficiency over time. Our system can be implemented using cheap, basic components, requires no specific technical expertise, and should be suitable for any non‐aquatic vascular plant species.https://doi.org/10.1002/pld3.571hydroponicsmodular phenotypingnoninvasive whole‐plant phenotypingosmotic stressSorghum bicolorwater use efficiency |
| spellingShingle | Daniel N. Ginzburg Jack A. Cox Seung Y. Rhee Non‐destructive, whole‐plant phenotyping reveals dynamic changes in water use efficiency, photosynthesis, and rhizosphere acidification of sorghum accessions under osmotic stress Plant Direct hydroponics modular phenotyping noninvasive whole‐plant phenotyping osmotic stress Sorghum bicolor water use efficiency |
| title | Non‐destructive, whole‐plant phenotyping reveals dynamic changes in water use efficiency, photosynthesis, and rhizosphere acidification of sorghum accessions under osmotic stress |
| title_full | Non‐destructive, whole‐plant phenotyping reveals dynamic changes in water use efficiency, photosynthesis, and rhizosphere acidification of sorghum accessions under osmotic stress |
| title_fullStr | Non‐destructive, whole‐plant phenotyping reveals dynamic changes in water use efficiency, photosynthesis, and rhizosphere acidification of sorghum accessions under osmotic stress |
| title_full_unstemmed | Non‐destructive, whole‐plant phenotyping reveals dynamic changes in water use efficiency, photosynthesis, and rhizosphere acidification of sorghum accessions under osmotic stress |
| title_short | Non‐destructive, whole‐plant phenotyping reveals dynamic changes in water use efficiency, photosynthesis, and rhizosphere acidification of sorghum accessions under osmotic stress |
| title_sort | non destructive whole plant phenotyping reveals dynamic changes in water use efficiency photosynthesis and rhizosphere acidification of sorghum accessions under osmotic stress |
| topic | hydroponics modular phenotyping noninvasive whole‐plant phenotyping osmotic stress Sorghum bicolor water use efficiency |
| url | https://doi.org/10.1002/pld3.571 |
| work_keys_str_mv | AT danielnginzburg nondestructivewholeplantphenotypingrevealsdynamicchangesinwateruseefficiencyphotosynthesisandrhizosphereacidificationofsorghumaccessionsunderosmoticstress AT jackacox nondestructivewholeplantphenotypingrevealsdynamicchangesinwateruseefficiencyphotosynthesisandrhizosphereacidificationofsorghumaccessionsunderosmoticstress AT seungyrhee nondestructivewholeplantphenotypingrevealsdynamicchangesinwateruseefficiencyphotosynthesisandrhizosphereacidificationofsorghumaccessionsunderosmoticstress |