High-Temperature, Nonradioactive Primer Extension Assay for Determination of a Transcription-Initiation Site
We have developed a simple and safe method for the determination of a transcription-initiation site. In this method, reverse transcriptase of the avian myeloblastosis virus or rTth DNA polymerase from Thermus thermopilus was used with a fluorescein isothiocyanate (FITC)-labeled primer. The primer-ex...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
1998-07-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/98251st02 |
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