First Report of ‘Candidatus Phytoplasma australasia’ Strain Related to Witches’-Broom of Tomato in Türkiye
Phytoplasmas are dangerous bacteria severely infecting agricultural production worldwide. In the present study, the identification of phytoplasmas infecting tomato plants showing symptoms such as small leaves, flower abnormalities, stunting, witches' broom, and reddening was performed. Fi...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Yuzuncu Yil University Faculty of Agriculture
2023-03-01
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| Series: | Yüzüncü Yıl Üniversitesi Tarim Bilimleri Dergisi |
| Subjects: | |
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| Summary: | Phytoplasmas are dangerous bacteria severely infecting agricultural
production worldwide. In the present study, the identification of phytoplasmas
infecting tomato plants showing symptoms such as small leaves, flower
abnormalities, stunting, witches' broom, and reddening was performed. Five
plants, two symptomatic and three asymptomatic, were tested to verify
phytoplasma infection. Total DNA isolated from 5 leaf samples was used as a
template for PCR reactions. The phytoplasma agents were confirmed in the two
symptomatic samples. BLASTn search of 16S rRNA of two sequences shared
identity similarity of 99.84% with ‘Candidatus Phytoplasma australasia’.
Computer-simulated virtual RFLP profiles show that the 16S rRNA sequences is
identical to the reference pattern of the 16SrII-D subgroup, with a similarity
coefficient of 1.00. Based on BLAST, virtual RFLP, and phylogenetic
dendrogram, the identified phytoplasma strains are enclosed in the 16SrII-D
subgroup. This is the first report of tomato witches' broom disease related to
16SrII-D subgroup phytoplasma strains in the Antalya province of Türkiye. |
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| ISSN: | 1308-7576 1308-7584 |