The Effect of Morinda citrifolia L. Fruit Extract Supplemented in to Diluent Tris-Egg Yolk Toward the Abnormality of Goat's Spermatozoa after Freezing at -80°C
The aim of this research is to know the effect of diluent Tris-egg yolk medium added with Morinda citrifolia L. extract towards the sperms abnormality after storage at -80°C for 24 hours. The extracts added in diluent Tris-egg yolk medium were 0% (P0), 10% (P1), 20% (P2), and 30% (P3). The result sh...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
University of Brawijaya
2018-10-01
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| Series: | Journal of Experimental Life Science |
| Subjects: | |
| Online Access: | https://jels.ub.ac.id/index.php/jels/article/view/286 |
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| Summary: | The aim of this research is to know the effect of diluent Tris-egg yolk medium added with Morinda citrifolia L. extract towards the sperms abnormality after storage at -80°C for 24 hours. The extracts added in diluent Tris-egg yolk medium were 0% (P0), 10% (P1), 20% (P2), and 30% (P3). The result showed that the percentage of sperms abnormality in P0, P1, P2, and P3 were respectively 8.67%, 6.67%, 5.67%, and 10%. P2 was the lowest percentage, while P3 was the highest percentage of sperms abnormality. In addition, this research found twelve kinds of sperms abnormality divided into categories: major abnormality included the broke tail, absent head, detached head, dag defect, tapered head, proximal droplet, round head and abaxial; and minor abnormality included the coiled tail, shoe hook tail, bent tail, and distal droplet. It has been concluded that the allotment of 20% Morinda citrifolia L. extracts in Tris-egg yolk medium able to maintain the sperm morphology at -80°C storage for 24 hours. |
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| ISSN: | 2087-2852 2338-1655 |