Isolation and Identification of Dominant Bacteria from Raw Donkey Milk Produced in a Region of Morocco by QIIME 2 and Evaluation of Their Antibacterial Activity
Recently, the interest in donkey milk has increased considerably because it proved high nutritive and functional values of their ingredients. Its chemical composition is widely studied, but its microbiota, especially lactic acid bacteria, remains less studied. This study focuses on analyzing, isolat...
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2021/6664636 |
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| author | Reda Derdak Javier Quinteiro Souraya Sakoui Boutaina Addoum Jorge Rodríguez Castro Manuel Rey Méndez Abdelaziz Soukri Bouchra El Khalfi |
| author_facet | Reda Derdak Javier Quinteiro Souraya Sakoui Boutaina Addoum Jorge Rodríguez Castro Manuel Rey Méndez Abdelaziz Soukri Bouchra El Khalfi |
| author_sort | Reda Derdak |
| collection | DOAJ |
| description | Recently, the interest in donkey milk has increased considerably because it proved high nutritive and functional values of their ingredients. Its chemical composition is widely studied, but its microbiota, especially lactic acid bacteria, remains less studied. This study focuses on analyzing, isolating, and identifying lactic acid bacteria and evaluating their capacity to produce biomolecules with antibacterial activity. Among 44 strains identified, 43 are Gram-positive, and most are catalase-negative and cocci-shaped. Five strains were selected to evaluate their antibacterial activity against Listeria monocytogenes, Staphylococcus aureus, and Escherichia coli. Different induction methods allowed to amplify the antibacterial effects against these pathogenic strains. |
| format | Article |
| id | doaj-art-a84a3337978f477f9da42f6a02170fb9 |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-a84a3337978f477f9da42f6a02170fb92025-02-03T05:44:09ZengWileyThe Scientific World Journal2356-61401537-744X2021-01-01202110.1155/2021/66646366664636Isolation and Identification of Dominant Bacteria from Raw Donkey Milk Produced in a Region of Morocco by QIIME 2 and Evaluation of Their Antibacterial ActivityReda Derdak0Javier Quinteiro1Souraya Sakoui2Boutaina Addoum3Jorge Rodríguez Castro4Manuel Rey Méndez5Abdelaziz Soukri6Bouchra El Khalfi7Laboratory of Physiopathology, Molecular Genetics & Biotechnology, Ain Chock Faculty of Sciences, Health and Biotechnology Research Center, Hassan II University of Casablanca, B.P 5366 Maarif, Casablanca, MoroccoLaboratory of Molecular Systematics, Department of Biochemistry & Molecular Biology, CIBUS University of Santiago de Compostela, Santiago, SpainLaboratory of Physiopathology, Molecular Genetics & Biotechnology, Ain Chock Faculty of Sciences, Health and Biotechnology Research Center, Hassan II University of Casablanca, B.P 5366 Maarif, Casablanca, MoroccoLaboratory of Physiopathology, Molecular Genetics & Biotechnology, Ain Chock Faculty of Sciences, Health and Biotechnology Research Center, Hassan II University of Casablanca, B.P 5366 Maarif, Casablanca, MoroccoLaboratory of Molecular Systematics, Department of Biochemistry & Molecular Biology, CIBUS University of Santiago de Compostela, Santiago, SpainLaboratory of Molecular Systematics, Department of Biochemistry & Molecular Biology, CIBUS University of Santiago de Compostela, Santiago, SpainLaboratory of Physiopathology, Molecular Genetics & Biotechnology, Ain Chock Faculty of Sciences, Health and Biotechnology Research Center, Hassan II University of Casablanca, B.P 5366 Maarif, Casablanca, MoroccoLaboratory of Physiopathology, Molecular Genetics & Biotechnology, Ain Chock Faculty of Sciences, Health and Biotechnology Research Center, Hassan II University of Casablanca, B.P 5366 Maarif, Casablanca, MoroccoRecently, the interest in donkey milk has increased considerably because it proved high nutritive and functional values of their ingredients. Its chemical composition is widely studied, but its microbiota, especially lactic acid bacteria, remains less studied. This study focuses on analyzing, isolating, and identifying lactic acid bacteria and evaluating their capacity to produce biomolecules with antibacterial activity. Among 44 strains identified, 43 are Gram-positive, and most are catalase-negative and cocci-shaped. Five strains were selected to evaluate their antibacterial activity against Listeria monocytogenes, Staphylococcus aureus, and Escherichia coli. Different induction methods allowed to amplify the antibacterial effects against these pathogenic strains.http://dx.doi.org/10.1155/2021/6664636 |
| spellingShingle | Reda Derdak Javier Quinteiro Souraya Sakoui Boutaina Addoum Jorge Rodríguez Castro Manuel Rey Méndez Abdelaziz Soukri Bouchra El Khalfi Isolation and Identification of Dominant Bacteria from Raw Donkey Milk Produced in a Region of Morocco by QIIME 2 and Evaluation of Their Antibacterial Activity The Scientific World Journal |
| title | Isolation and Identification of Dominant Bacteria from Raw Donkey Milk Produced in a Region of Morocco by QIIME 2 and Evaluation of Their Antibacterial Activity |
| title_full | Isolation and Identification of Dominant Bacteria from Raw Donkey Milk Produced in a Region of Morocco by QIIME 2 and Evaluation of Their Antibacterial Activity |
| title_fullStr | Isolation and Identification of Dominant Bacteria from Raw Donkey Milk Produced in a Region of Morocco by QIIME 2 and Evaluation of Their Antibacterial Activity |
| title_full_unstemmed | Isolation and Identification of Dominant Bacteria from Raw Donkey Milk Produced in a Region of Morocco by QIIME 2 and Evaluation of Their Antibacterial Activity |
| title_short | Isolation and Identification of Dominant Bacteria from Raw Donkey Milk Produced in a Region of Morocco by QIIME 2 and Evaluation of Their Antibacterial Activity |
| title_sort | isolation and identification of dominant bacteria from raw donkey milk produced in a region of morocco by qiime 2 and evaluation of their antibacterial activity |
| url | http://dx.doi.org/10.1155/2021/6664636 |
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