Expression of genes of cytokines, transcription factors and differentiation antigens in human dendritic cells activated by double-stranded DNA
One of the most important properties of extracellular double-stranded DNA related to the treatment of various diseases is its ability to activate effector cells of the immune system (anti-tumor and vaccinal immunity) through dendritic cells (DCs). The stimulatory effect of DNA on DCs is mediated by th...
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| Format: | Article |
| Language: | English |
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Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
2017-11-01
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| Series: | Вавиловский журнал генетики и селекции |
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| Online Access: | https://vavilov.elpub.ru/jour/article/view/1193 |
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| author | A. S. Proskurina K. E. Orishchenko E. A. Potter E. V. Dolgova A. V. Spaselnikova G. S. Ritter N. A. Varaksin T. G. Ryabicheva O. Y. Leplina A. A. Ostanin E. R. Chernykh S. S. Bogachev |
| author_facet | A. S. Proskurina K. E. Orishchenko E. A. Potter E. V. Dolgova A. V. Spaselnikova G. S. Ritter N. A. Varaksin T. G. Ryabicheva O. Y. Leplina A. A. Ostanin E. R. Chernykh S. S. Bogachev |
| author_sort | A. S. Proskurina |
| collection | DOAJ |
| description | One of the most important properties of extracellular double-stranded DNA related to the treatment of various diseases is its ability to activate effector cells of the immune system (anti-tumor and vaccinal immunity) through dendritic cells (DCs). The stimulatory effect of DNA on DCs is mediated by the TLR9 signaling pathway and/or through a system of cytosolic sensors and is manifested by increased expression of MHC class II antigens and costimulatory molecules and by increased synthesis of immunoregulatory cytokines. In this work, the expression of cytokines, differentiation antigens and transcription factor genes has been investigated in DCs activated by double-stranded human DNA (i) without any additional factors, (ii) using a lipophilic agent, and (iii) by blocking TLR9 with chloroquine. Evaluation of the DNA effect was carried out after the 6- and 24-hour exposure. It was found that the preparation of double-stranded DNA transfected by Lipofectamine 2000 boosts DCs at the same level as Poly(dA : dT), a synthetic equivalent of double-stranded DNA. It was discovered that combined application of DNA and chloroquine enhances expression of the IFN-α, IFN-β, IFN-γ, IL8, МСР1, VEGF, CD25, and CD83 genes by hour 24 of incubation. It was for the first time shown that genomic “self” double-stranded DNA as a mono agent activates mRNA synthesis of cytokines IFN-α, IFN-β, IFN-γ, IL8, IL10, and VEGF in DCs at 6 hours of induction. |
| format | Article |
| id | doaj-art-a841dab9a9f8446d87560c2fbd89af1f |
| institution | Kabale University |
| issn | 2500-3259 |
| language | English |
| publishDate | 2017-11-01 |
| publisher | Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders |
| record_format | Article |
| series | Вавиловский журнал генетики и селекции |
| spelling | doaj-art-a841dab9a9f8446d87560c2fbd89af1f2025-02-01T09:58:04ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592017-11-0121671772710.18699/VJ17.289674Expression of genes of cytokines, transcription factors and differentiation antigens in human dendritic cells activated by double-stranded DNAA. S. Proskurina0K. E. Orishchenko1E. A. Potter2E. V. Dolgova3A. V. Spaselnikova4G. S. Ritter5N. A. Varaksin6T. G. Ryabicheva7O. Y. Leplina8A. A. Ostanin9E. R. Chernykh10S. S. Bogachev11Institute of Cytology and Genetics SB RAS, NovosibirskInstitute of Cytology and Genetics SB RAS, NovosibirskInstitute of Cytology and Genetics SB RAS, NovosibirskInstitute of Cytology and Genetics SB RAS, NovosibirskInstitute of Cytology and Genetics SB RAS, Novosibirsk; Novosibirsk State University, NovosibirskInstitute of Cytology and Genetics SB RAS, Novosibirsk; Novosibirsk State University, NovosibirskState Research Center of Virology and Biotechnology “Vector”, Koltsovo, Novosibirsk regionState Research Center of Virology and Biotechnology “Vector”, Koltsovo, Novosibirsk regionInstitute of Fundamental and Clinical Immunology, NovosibirskInstitute of Fundamental and Clinical Immunology, NovosibirskInstitute of Fundamental and Clinical Immunology, NovosibirskInstitute of Cytology and Genetics SB RAS, NovosibirskOne of the most important properties of extracellular double-stranded DNA related to the treatment of various diseases is its ability to activate effector cells of the immune system (anti-tumor and vaccinal immunity) through dendritic cells (DCs). The stimulatory effect of DNA on DCs is mediated by the TLR9 signaling pathway and/or through a system of cytosolic sensors and is manifested by increased expression of MHC class II antigens and costimulatory molecules and by increased synthesis of immunoregulatory cytokines. In this work, the expression of cytokines, differentiation antigens and transcription factor genes has been investigated in DCs activated by double-stranded human DNA (i) without any additional factors, (ii) using a lipophilic agent, and (iii) by blocking TLR9 with chloroquine. Evaluation of the DNA effect was carried out after the 6- and 24-hour exposure. It was found that the preparation of double-stranded DNA transfected by Lipofectamine 2000 boosts DCs at the same level as Poly(dA : dT), a synthetic equivalent of double-stranded DNA. It was discovered that combined application of DNA and chloroquine enhances expression of the IFN-α, IFN-β, IFN-γ, IL8, МСР1, VEGF, CD25, and CD83 genes by hour 24 of incubation. It was for the first time shown that genomic “self” double-stranded DNA as a mono agent activates mRNA synthesis of cytokines IFN-α, IFN-β, IFN-γ, IL8, IL10, and VEGF in DCs at 6 hours of induction.https://vavilov.elpub.ru/jour/article/view/1193chloroquinedouble-stranded dnacyto-kinesdendritic cellsreal-time pcr |
| spellingShingle | A. S. Proskurina K. E. Orishchenko E. A. Potter E. V. Dolgova A. V. Spaselnikova G. S. Ritter N. A. Varaksin T. G. Ryabicheva O. Y. Leplina A. A. Ostanin E. R. Chernykh S. S. Bogachev Expression of genes of cytokines, transcription factors and differentiation antigens in human dendritic cells activated by double-stranded DNA Вавиловский журнал генетики и селекции chloroquine double-stranded dna cyto-kines dendritic cells real-time pcr |
| title | Expression of genes of cytokines, transcription factors and differentiation antigens in human dendritic cells activated by double-stranded DNA |
| title_full | Expression of genes of cytokines, transcription factors and differentiation antigens in human dendritic cells activated by double-stranded DNA |
| title_fullStr | Expression of genes of cytokines, transcription factors and differentiation antigens in human dendritic cells activated by double-stranded DNA |
| title_full_unstemmed | Expression of genes of cytokines, transcription factors and differentiation antigens in human dendritic cells activated by double-stranded DNA |
| title_short | Expression of genes of cytokines, transcription factors and differentiation antigens in human dendritic cells activated by double-stranded DNA |
| title_sort | expression of genes of cytokines transcription factors and differentiation antigens in human dendritic cells activated by double stranded dna |
| topic | chloroquine double-stranded dna cyto-kines dendritic cells real-time pcr |
| url | https://vavilov.elpub.ru/jour/article/view/1193 |
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