High Spatiotemporal Near‐Infrared II Fluorescence Lifetime Imaging for Quantitative Detection of Clinical Tumor Margins
Abstract Accurate detection of tumor margins is essential for successful cancer surgery. While indocyanine green (ICG)‐based near‐infrared (NIR) fluorescence (FL) surgical navigation enhances the visual identification of tumor margins, its accuracy remains subjective, underscoring the need for quant...
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| Format: | Article |
| Language: | English |
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Wiley
2025-02-01
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| Series: | Advanced Science |
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| Online Access: | https://doi.org/10.1002/advs.202411272 |
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| author | Zhen Chen Linjian Huang Duyang Gao Zhouzhou Bao Dehong Hu Wei Zheng Jing Chen Jiuling Liao Hairong Zheng Zonghai Sheng |
| author_facet | Zhen Chen Linjian Huang Duyang Gao Zhouzhou Bao Dehong Hu Wei Zheng Jing Chen Jiuling Liao Hairong Zheng Zonghai Sheng |
| author_sort | Zhen Chen |
| collection | DOAJ |
| description | Abstract Accurate detection of tumor margins is essential for successful cancer surgery. While indocyanine green (ICG)‐based near‐infrared (NIR) fluorescence (FL) surgical navigation enhances the visual identification of tumor margins, its accuracy remains subjective, underscoring the need for quantitative approaches. In this study, a high spatiotemporal fluorescence lifetime (FLT) imaging technology is developed in the second near‐infrared window (NIR‐II, 1000–1700 nm) for quantitative tumor margin detection, utilizing folate receptor‐targeted ICG nanoprobes (FPH‐ICG). FPH‐ICG exhibits a significantly prolonged NIR‐II FLT (750 ± 7 ps vs 260 ± 3 ps) and increased NIR‐II FL brightness (FPH‐ICG/ICG = 3.8). In vitro and in vivo studies confirm that FPH‐ICG specifically targets folate receptor‐α (FRα) on SK‐OV‐3 ovarian cancer cells, achieving high‐contrast NIR‐II FL imaging with a signal‐to‐background ratio of 10.8. Notably, NIR‐II FLT imaging demonstrates superior accuracy (90%) and consistency in defining tumor margins compared to NIR‐II FL imaging (58%) in both SK‐OV‐3 tumor‐bearing mice and clinical tumor samples. These findings underscore the potential of NIR‐II FLT imaging as a quantitative tool for guiding surgical tumor margin detection. |
| format | Article |
| id | doaj-art-a80dd0b7e87b495e98878d8782989ff0 |
| institution | Kabale University |
| issn | 2198-3844 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj-art-a80dd0b7e87b495e98878d8782989ff02025-02-04T13:14:54ZengWileyAdvanced Science2198-38442025-02-01125n/an/a10.1002/advs.202411272High Spatiotemporal Near‐Infrared II Fluorescence Lifetime Imaging for Quantitative Detection of Clinical Tumor MarginsZhen Chen0Linjian Huang1Duyang Gao2Zhouzhou Bao3Dehong Hu4Wei Zheng5Jing Chen6Jiuling Liao7Hairong Zheng8Zonghai Sheng9State Key Laboratory of Radio Frequency Heterogeneous Integration College of Electronics and Information Engineering Shenzhen University Shenzhen 518060 P. R. ChinaResearch Center for Biomedical Optics and Molecular Imaging Shenzhen Key Laboratory for Molecular Imaging Guangdong Provincial Key Laboratory of Biomedical Optical Imaging Technology Key Laboratory of Biomedical Imaging Science and System Shenzhen Institute of Advanced Technology Chinese Academy of Sciences Shenzhen 518055 P. R. ChinaResearch Center for Advanced Detection Materials and Medical Imaging Devices Paul C. Lauterbur Research Center for Biomedical Imaging Institute of Biomedical and Health Engineering Shenzhen Institute of Advanced Technology Chinese Academy of Sciences Shenzhen 518055 P. R. ChinaDepartment of Obstetrics and Gynecology Shanghai Key Laboratory of Gynecologic Oncology Ren Ji Hospital School of Medicine Shanghai Jiao Tong University Shanghai 200127 P. R. ChinaResearch Center for Advanced Detection Materials and Medical Imaging Devices Paul C. Lauterbur Research Center for Biomedical Imaging Institute of Biomedical and Health Engineering Shenzhen Institute of Advanced Technology Chinese Academy of Sciences Shenzhen 518055 P. R. ChinaResearch Center for Biomedical Optics and Molecular Imaging Shenzhen Key Laboratory for Molecular Imaging Guangdong Provincial Key Laboratory of Biomedical Optical Imaging Technology Key Laboratory of Biomedical Imaging Science and System Shenzhen Institute of Advanced Technology Chinese Academy of Sciences Shenzhen 518055 P. R. ChinaState Key Laboratory of Radio Frequency Heterogeneous Integration College of Electronics and Information Engineering Shenzhen University Shenzhen 518060 P. R. ChinaResearch Center for Biomedical Optics and Molecular Imaging Shenzhen Key Laboratory for Molecular Imaging Guangdong Provincial Key Laboratory of Biomedical Optical Imaging Technology Key Laboratory of Biomedical Imaging Science and System Shenzhen Institute of Advanced Technology Chinese Academy of Sciences Shenzhen 518055 P. R. ChinaResearch Center for Advanced Detection Materials and Medical Imaging Devices Paul C. Lauterbur Research Center for Biomedical Imaging Institute of Biomedical and Health Engineering Shenzhen Institute of Advanced Technology Chinese Academy of Sciences Shenzhen 518055 P. R. ChinaResearch Center for Advanced Detection Materials and Medical Imaging Devices Paul C. Lauterbur Research Center for Biomedical Imaging Institute of Biomedical and Health Engineering Shenzhen Institute of Advanced Technology Chinese Academy of Sciences Shenzhen 518055 P. R. ChinaAbstract Accurate detection of tumor margins is essential for successful cancer surgery. While indocyanine green (ICG)‐based near‐infrared (NIR) fluorescence (FL) surgical navigation enhances the visual identification of tumor margins, its accuracy remains subjective, underscoring the need for quantitative approaches. In this study, a high spatiotemporal fluorescence lifetime (FLT) imaging technology is developed in the second near‐infrared window (NIR‐II, 1000–1700 nm) for quantitative tumor margin detection, utilizing folate receptor‐targeted ICG nanoprobes (FPH‐ICG). FPH‐ICG exhibits a significantly prolonged NIR‐II FLT (750 ± 7 ps vs 260 ± 3 ps) and increased NIR‐II FL brightness (FPH‐ICG/ICG = 3.8). In vitro and in vivo studies confirm that FPH‐ICG specifically targets folate receptor‐α (FRα) on SK‐OV‐3 ovarian cancer cells, achieving high‐contrast NIR‐II FL imaging with a signal‐to‐background ratio of 10.8. Notably, NIR‐II FLT imaging demonstrates superior accuracy (90%) and consistency in defining tumor margins compared to NIR‐II FL imaging (58%) in both SK‐OV‐3 tumor‐bearing mice and clinical tumor samples. These findings underscore the potential of NIR‐II FLT imaging as a quantitative tool for guiding surgical tumor margin detection.https://doi.org/10.1002/advs.202411272indocyanine greenmolecular targetnear‐infrared‐II fluorescence lifetimetumor margin |
| spellingShingle | Zhen Chen Linjian Huang Duyang Gao Zhouzhou Bao Dehong Hu Wei Zheng Jing Chen Jiuling Liao Hairong Zheng Zonghai Sheng High Spatiotemporal Near‐Infrared II Fluorescence Lifetime Imaging for Quantitative Detection of Clinical Tumor Margins Advanced Science indocyanine green molecular target near‐infrared‐II fluorescence lifetime tumor margin |
| title | High Spatiotemporal Near‐Infrared II Fluorescence Lifetime Imaging for Quantitative Detection of Clinical Tumor Margins |
| title_full | High Spatiotemporal Near‐Infrared II Fluorescence Lifetime Imaging for Quantitative Detection of Clinical Tumor Margins |
| title_fullStr | High Spatiotemporal Near‐Infrared II Fluorescence Lifetime Imaging for Quantitative Detection of Clinical Tumor Margins |
| title_full_unstemmed | High Spatiotemporal Near‐Infrared II Fluorescence Lifetime Imaging for Quantitative Detection of Clinical Tumor Margins |
| title_short | High Spatiotemporal Near‐Infrared II Fluorescence Lifetime Imaging for Quantitative Detection of Clinical Tumor Margins |
| title_sort | high spatiotemporal near infrared ii fluorescence lifetime imaging for quantitative detection of clinical tumor margins |
| topic | indocyanine green molecular target near‐infrared‐II fluorescence lifetime tumor margin |
| url | https://doi.org/10.1002/advs.202411272 |
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