Ascospore discharge, germination and culture of fungal partners of tropical lichens, including the use of a novel culture technique
A total of 292 lichen samples, representing over 200 species and at least 65 genera and 26 families, were collected, mainly in Thailand; 170 of the specimens discharged ascospores in the laboratory. Generally, crustose lichens exhibited the highest discharge rates and percentage germination. In con...
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| Format: | Article |
| Language: | English |
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BMC
2011-12-01
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| Series: | IMA Fungus |
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| Online Access: | http://www.ingentaconnect.com/content/ima/imafung/2011/00000002/00000002/art00012 |
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| author | E. Sangvichien D.L. Hawksworth A.J.S. Whalley |
| author_facet | E. Sangvichien D.L. Hawksworth A.J.S. Whalley |
| author_sort | E. Sangvichien |
| collection | DOAJ |
| description | A total of 292 lichen samples, representing over 200 species and at least 65 genera and 26 families, were collected, mainly in Thailand; 170 of the specimens discharged ascospores in the laboratory. Generally, crustose lichens exhibited the highest discharge rates and percentage germination. In contrast, foliose lichen samples, although having a high discharge rate, had a lower percentage germination than crustose species tested. A correlation with season was indicated for a number of species. Continued development of germinated ascospores into recognizable colonies in pure culture was followed for a selection of species. The most successful medium tried was 2 % Malt-Yeast extract agar (MYA), and under static conditions using a liquid culture medium, a sponge proved to be the best of several physical carriers tested; this novel method has considerable potential for experimental work with lichen mycobionts. |
| format | Article |
| id | doaj-art-a80bccd0a233488fb523fb57a098988e |
| institution | Kabale University |
| issn | 2210-6340 2210-6359 |
| language | English |
| publishDate | 2011-12-01 |
| publisher | BMC |
| record_format | Article |
| series | IMA Fungus |
| spelling | doaj-art-a80bccd0a233488fb523fb57a098988e2025-02-03T00:05:12ZengBMCIMA Fungus2210-63402210-63592011-12-0122143153Ascospore discharge, germination and culture of fungal partners of tropical lichens, including the use of a novel culture techniqueE. SangvichienD.L. HawksworthA.J.S. WhalleyA total of 292 lichen samples, representing over 200 species and at least 65 genera and 26 families, were collected, mainly in Thailand; 170 of the specimens discharged ascospores in the laboratory. Generally, crustose lichens exhibited the highest discharge rates and percentage germination. In contrast, foliose lichen samples, although having a high discharge rate, had a lower percentage germination than crustose species tested. A correlation with season was indicated for a number of species. Continued development of germinated ascospores into recognizable colonies in pure culture was followed for a selection of species. The most successful medium tried was 2 % Malt-Yeast extract agar (MYA), and under static conditions using a liquid culture medium, a sponge proved to be the best of several physical carriers tested; this novel method has considerable potential for experimental work with lichen mycobionts.http://www.ingentaconnect.com/content/ima/imafung/2011/00000002/00000002/art00012Ascomycotacolony developmentmycobiontseasonalityThailand |
| spellingShingle | E. Sangvichien D.L. Hawksworth A.J.S. Whalley Ascospore discharge, germination and culture of fungal partners of tropical lichens, including the use of a novel culture technique IMA Fungus Ascomycota colony development mycobiont seasonality Thailand |
| title | Ascospore discharge, germination and culture of fungal partners of tropical lichens, including the use of a novel culture technique |
| title_full | Ascospore discharge, germination and culture of fungal partners of tropical lichens, including the use of a novel culture technique |
| title_fullStr | Ascospore discharge, germination and culture of fungal partners of tropical lichens, including the use of a novel culture technique |
| title_full_unstemmed | Ascospore discharge, germination and culture of fungal partners of tropical lichens, including the use of a novel culture technique |
| title_short | Ascospore discharge, germination and culture of fungal partners of tropical lichens, including the use of a novel culture technique |
| title_sort | ascospore discharge germination and culture of fungal partners of tropical lichens including the use of a novel culture technique |
| topic | Ascomycota colony development mycobiont seasonality Thailand |
| url | http://www.ingentaconnect.com/content/ima/imafung/2011/00000002/00000002/art00012 |
| work_keys_str_mv | AT esangvichien ascosporedischargegerminationandcultureoffungalpartnersoftropicallichensincludingtheuseofanovelculturetechnique AT dlhawksworth ascosporedischargegerminationandcultureoffungalpartnersoftropicallichensincludingtheuseofanovelculturetechnique AT ajswhalley ascosporedischargegerminationandcultureoffungalpartnersoftropicallichensincludingtheuseofanovelculturetechnique |