Circulating C19MC MicroRNAs in Preeclampsia, Gestational Hypertension, and Fetal Growth Restriction
The objective of the study was to identify the profile of circulating C19MC microRNAs (miR-516-5p, miR-517*, miR-518b, miR-520a*, miR-520h, miR-525, and miR-526a) in patients with established preeclampsia (n=63), fetal growth restriction (n=27), and gestational hypertension (n=23). We examined the c...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2013/186041 |
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| author | Ilona Hromadnikova Katerina Kotlabova Marketa Ondrackova Andrea Kestlerova Veronika Novotna Lucie Hympanova Jindrich Doucha Ladislav Krofta |
| author_facet | Ilona Hromadnikova Katerina Kotlabova Marketa Ondrackova Andrea Kestlerova Veronika Novotna Lucie Hympanova Jindrich Doucha Ladislav Krofta |
| author_sort | Ilona Hromadnikova |
| collection | DOAJ |
| description | The objective of the study was to identify the profile of circulating C19MC microRNAs (miR-516-5p, miR-517*, miR-518b, miR-520a*, miR-520h, miR-525, and miR-526a) in patients with established preeclampsia (n=63), fetal growth restriction (n=27), and gestational hypertension (n=23). We examined the correlation between plasmatic concentrations and expression levels of microRNAs and the severity of the disease with respect to clinical signs, requirements for the delivery, and Doppler ultrasound parameters. Using absolute and relative quantification approaches, increased extracellular C19MC microRNA levels (miR-516-5p, P=0.037, P=0.009; miR-517*, P=0.033, P=0.043; miR-520a*, P=0.001, P=0.009; miR-525, P=0.026, P=0.01; miR-526a, P=0.03, P=0.035) were detected in patients with preeclampsia. The association analysis pointed to no relationship between C19MC microRNA plasmatic concentrations and expression profile and identified risk factors for a poorer perinatal outcome. However, the dependence between the levels of plasmatic C19MC microRNAs and the pulsatility index in the middle cerebral artery and the values of cerebroplacental ratio was demonstrated. The study brought the interesting finding that the upregulation of miR-516-5p, miR-517*, miR-520a*, miR-525, and miR-526a is a characteristic phenomenon of established preeclampsia. |
| format | Article |
| id | doaj-art-a7fb30c3c7df4e42bae5bf8cd6616721 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-a7fb30c3c7df4e42bae5bf8cd66167212025-02-03T01:09:08ZengWileyMediators of Inflammation0962-93511466-18612013-01-01201310.1155/2013/186041186041Circulating C19MC MicroRNAs in Preeclampsia, Gestational Hypertension, and Fetal Growth RestrictionIlona Hromadnikova0Katerina Kotlabova1Marketa Ondrackova2Andrea Kestlerova3Veronika Novotna4Lucie Hympanova5Jindrich Doucha6Ladislav Krofta7Department of Molecular Biology and Cell Pathology, Third Faculty of Medicine, Charles University, Ruska 87, 100 00 Prague, Czech RepublicDepartment of Molecular Biology and Cell Pathology, Third Faculty of Medicine, Charles University, Ruska 87, 100 00 Prague, Czech RepublicDepartment of Molecular Biology and Cell Pathology, Third Faculty of Medicine, Charles University, Ruska 87, 100 00 Prague, Czech RepublicInstitute for the Care of the Mother and Child, Third Faculty of Medicine, Charles University, Podolske Nabrezi 157/36, 147 00 Prague, Czech RepublicInstitute for the Care of the Mother and Child, Third Faculty of Medicine, Charles University, Podolske Nabrezi 157/36, 147 00 Prague, Czech RepublicDepartment of Molecular Biology and Cell Pathology, Third Faculty of Medicine, Charles University, Ruska 87, 100 00 Prague, Czech RepublicClinic of Obstetrics and Gynecology, Second Faculty of Medicine, Charles University, V Uvalu 84, 150 06 Prague, Czech RepublicInstitute for the Care of the Mother and Child, Third Faculty of Medicine, Charles University, Podolske Nabrezi 157/36, 147 00 Prague, Czech RepublicThe objective of the study was to identify the profile of circulating C19MC microRNAs (miR-516-5p, miR-517*, miR-518b, miR-520a*, miR-520h, miR-525, and miR-526a) in patients with established preeclampsia (n=63), fetal growth restriction (n=27), and gestational hypertension (n=23). We examined the correlation between plasmatic concentrations and expression levels of microRNAs and the severity of the disease with respect to clinical signs, requirements for the delivery, and Doppler ultrasound parameters. Using absolute and relative quantification approaches, increased extracellular C19MC microRNA levels (miR-516-5p, P=0.037, P=0.009; miR-517*, P=0.033, P=0.043; miR-520a*, P=0.001, P=0.009; miR-525, P=0.026, P=0.01; miR-526a, P=0.03, P=0.035) were detected in patients with preeclampsia. The association analysis pointed to no relationship between C19MC microRNA plasmatic concentrations and expression profile and identified risk factors for a poorer perinatal outcome. However, the dependence between the levels of plasmatic C19MC microRNAs and the pulsatility index in the middle cerebral artery and the values of cerebroplacental ratio was demonstrated. The study brought the interesting finding that the upregulation of miR-516-5p, miR-517*, miR-520a*, miR-525, and miR-526a is a characteristic phenomenon of established preeclampsia.http://dx.doi.org/10.1155/2013/186041 |
| spellingShingle | Ilona Hromadnikova Katerina Kotlabova Marketa Ondrackova Andrea Kestlerova Veronika Novotna Lucie Hympanova Jindrich Doucha Ladislav Krofta Circulating C19MC MicroRNAs in Preeclampsia, Gestational Hypertension, and Fetal Growth Restriction Mediators of Inflammation |
| title | Circulating C19MC MicroRNAs in Preeclampsia, Gestational Hypertension, and Fetal Growth Restriction |
| title_full | Circulating C19MC MicroRNAs in Preeclampsia, Gestational Hypertension, and Fetal Growth Restriction |
| title_fullStr | Circulating C19MC MicroRNAs in Preeclampsia, Gestational Hypertension, and Fetal Growth Restriction |
| title_full_unstemmed | Circulating C19MC MicroRNAs in Preeclampsia, Gestational Hypertension, and Fetal Growth Restriction |
| title_short | Circulating C19MC MicroRNAs in Preeclampsia, Gestational Hypertension, and Fetal Growth Restriction |
| title_sort | circulating c19mc micrornas in preeclampsia gestational hypertension and fetal growth restriction |
| url | http://dx.doi.org/10.1155/2013/186041 |
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