Temporal Changes in Gene Expression Profile during Mature Adipocyte Dedifferentiation
Objective. To characterize changes in gene expression profile during human mature adipocyte dedifferentiation in ceiling culture. Methods. Subcutaneous (SC) and omental (OM) adipose tissue samples were obtained from 4 participants paired for age and BMI. Isolated adipocytes were dedifferentiated in...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | International Journal of Genomics |
| Online Access: | http://dx.doi.org/10.1155/2017/5149362 |
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| author | Julie Anne Côté Frédéric Guénard Julie Lessard Marc Lapointe Simon Biron Marie-Claude Vohl André Tchernof |
| author_facet | Julie Anne Côté Frédéric Guénard Julie Lessard Marc Lapointe Simon Biron Marie-Claude Vohl André Tchernof |
| author_sort | Julie Anne Côté |
| collection | DOAJ |
| description | Objective. To characterize changes in gene expression profile during human mature adipocyte dedifferentiation in ceiling culture. Methods. Subcutaneous (SC) and omental (OM) adipose tissue samples were obtained from 4 participants paired for age and BMI. Isolated adipocytes were dedifferentiated in ceiling culture. Gene expression analysis at days 0, 4, 7, and 12 of the cultures was performed using Affymetrix Human Gene 2.0 STvi arrays. Hierarchical clustering according to similarity of expression changes was used to identify overrepresented functions. Results. Four clusters gathered genes with similar expression between day 4 to day 7 but decreasing expression from day 7 to day 12. Most of these genes coded for proteins involved in adipocyte functions (LIPE, PLIN1, DGAT2, PNPLA2, ADIPOQ, CEBPA, LPL, FABP4, SCD, INSR, and LEP). Expression of several genes coding for proteins implicated in cellular proliferation and growth or cell cycle increased significantly from day 7 to day 12 (WNT5A, KITLG, and FGF5). Genes coding for extracellular matrix proteins were differentially expressed between days 0, 4, 7, and 12 (COL1A1, COL1A2, and COL6A3, MMP1, and TGFB1). Conclusion. Dedifferentiation is associated with downregulation of transcripts encoding proteins involved in mature adipocyte functions and upregulation of genes involved in matrix remodeling, cellular development, and cell cycle. |
| format | Article |
| id | doaj-art-a7e3de27be544b4a840d0641b7fa1038 |
| institution | Kabale University |
| issn | 2314-436X 2314-4378 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Genomics |
| spelling | doaj-art-a7e3de27be544b4a840d0641b7fa10382025-02-03T01:32:39ZengWileyInternational Journal of Genomics2314-436X2314-43782017-01-01201710.1155/2017/51493625149362Temporal Changes in Gene Expression Profile during Mature Adipocyte DedifferentiationJulie Anne Côté0Frédéric Guénard1Julie Lessard2Marc Lapointe3Simon Biron4Marie-Claude Vohl5André Tchernof6Centre de Recherche de l’Institut Universitaire de Cardiologie et de Pneumologie de Québec, 2725 Chemin Ste-Foy, Quebec City, QC, G1V 4G5, CanadaÉcole de Nutrition, Université Laval, 2425 Rue de l’Agriculture, Quebec City, QC, G1V 0A6, CanadaCentre de Recherche de l’Institut Universitaire de Cardiologie et de Pneumologie de Québec, 2725 Chemin Ste-Foy, Quebec City, QC, G1V 4G5, CanadaCentre de Recherche de l’Institut Universitaire de Cardiologie et de Pneumologie de Québec, 2725 Chemin Ste-Foy, Quebec City, QC, G1V 4G5, CanadaCentre de Recherche de l’Institut Universitaire de Cardiologie et de Pneumologie de Québec, 2725 Chemin Ste-Foy, Quebec City, QC, G1V 4G5, CanadaÉcole de Nutrition, Université Laval, 2425 Rue de l’Agriculture, Quebec City, QC, G1V 0A6, CanadaCentre de Recherche de l’Institut Universitaire de Cardiologie et de Pneumologie de Québec, 2725 Chemin Ste-Foy, Quebec City, QC, G1V 4G5, CanadaObjective. To characterize changes in gene expression profile during human mature adipocyte dedifferentiation in ceiling culture. Methods. Subcutaneous (SC) and omental (OM) adipose tissue samples were obtained from 4 participants paired for age and BMI. Isolated adipocytes were dedifferentiated in ceiling culture. Gene expression analysis at days 0, 4, 7, and 12 of the cultures was performed using Affymetrix Human Gene 2.0 STvi arrays. Hierarchical clustering according to similarity of expression changes was used to identify overrepresented functions. Results. Four clusters gathered genes with similar expression between day 4 to day 7 but decreasing expression from day 7 to day 12. Most of these genes coded for proteins involved in adipocyte functions (LIPE, PLIN1, DGAT2, PNPLA2, ADIPOQ, CEBPA, LPL, FABP4, SCD, INSR, and LEP). Expression of several genes coding for proteins implicated in cellular proliferation and growth or cell cycle increased significantly from day 7 to day 12 (WNT5A, KITLG, and FGF5). Genes coding for extracellular matrix proteins were differentially expressed between days 0, 4, 7, and 12 (COL1A1, COL1A2, and COL6A3, MMP1, and TGFB1). Conclusion. Dedifferentiation is associated with downregulation of transcripts encoding proteins involved in mature adipocyte functions and upregulation of genes involved in matrix remodeling, cellular development, and cell cycle.http://dx.doi.org/10.1155/2017/5149362 |
| spellingShingle | Julie Anne Côté Frédéric Guénard Julie Lessard Marc Lapointe Simon Biron Marie-Claude Vohl André Tchernof Temporal Changes in Gene Expression Profile during Mature Adipocyte Dedifferentiation International Journal of Genomics |
| title | Temporal Changes in Gene Expression Profile during Mature Adipocyte Dedifferentiation |
| title_full | Temporal Changes in Gene Expression Profile during Mature Adipocyte Dedifferentiation |
| title_fullStr | Temporal Changes in Gene Expression Profile during Mature Adipocyte Dedifferentiation |
| title_full_unstemmed | Temporal Changes in Gene Expression Profile during Mature Adipocyte Dedifferentiation |
| title_short | Temporal Changes in Gene Expression Profile during Mature Adipocyte Dedifferentiation |
| title_sort | temporal changes in gene expression profile during mature adipocyte dedifferentiation |
| url | http://dx.doi.org/10.1155/2017/5149362 |
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