De novo transcriptome profiling revealing genes involved in piperine biosynthetic pathway in Piper longum L
Abstract Piper longum, commonly known as long pepper, is highly valued for its bioactive alkaloid piperine, which has diverse pharmaceutical and culinary applications. In this study, we used high-throughput sequencing and de novo transcriptome assembly to analyze the transcriptomes of P. longum leav...
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Nature Portfolio
2025-01-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-025-87434-5 |
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| author | Mrinalini Prasad Shivangi Mathur Deeksha Singh Rajiv Ranjan |
| author_facet | Mrinalini Prasad Shivangi Mathur Deeksha Singh Rajiv Ranjan |
| author_sort | Mrinalini Prasad |
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| description | Abstract Piper longum, commonly known as long pepper, is highly valued for its bioactive alkaloid piperine, which has diverse pharmaceutical and culinary applications. In this study, we used high-throughput sequencing and de novo transcriptome assembly to analyze the transcriptomes of P. longum leaves, roots, and spikes. Our dataset consisted of 173,381 high-quality transcripts, with functional annotations highlighting key pathways involved in lysine biosynthesis and secondary metabolite production. We identified 8041 simple sequence repeats (SSRs), primarily trinucleotide repeats, adding valuable genetic markers. Additionally, we uncovered 21,235 transcription factors (TFs) across 65 families, including bHLH, NAC, and MYB, which are essential in regulating metabolism. KEGG pathway analysis categorized 4730 transcripts into 377 pathways, focusing on piperine biosynthesis, including phenylpropanoid and L-lysine metabolism. Our research identified 14 expressed genes encoding enzymes crucial for tropane, piperidine, and pyridine alkaloid biosynthesis, shedding light on piperine production mechanisms. High-performance liquid chromatography (HPLC) revealed varying piperine concentrations, with spikes showing the highest content, followed by roots and leaves. This comprehensive analysis of the P. longum transcriptome offers valuable insights into the genetics and regulatory networks underlying piperine biosynthesis, identifying molecular markers and genes that hold significant potential for enhancing the plant’s biological and pharmaceutical applications. |
| format | Article |
| id | doaj-art-a7d08538b3c547e0923f7620dcd9993c |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
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| spelling | doaj-art-a7d08538b3c547e0923f7620dcd9993c2025-01-26T12:24:58ZengNature PortfolioScientific Reports2045-23222025-01-0115111510.1038/s41598-025-87434-5De novo transcriptome profiling revealing genes involved in piperine biosynthetic pathway in Piper longum LMrinalini Prasad0Shivangi Mathur1Deeksha Singh2Rajiv Ranjan3Plant Biotechnology Lab, Department of Botany, Faculty of Science, Dayalbagh Educational Institute (Deemed to be University)Plant Biotechnology Lab, Department of Botany, Faculty of Science, Dayalbagh Educational Institute (Deemed to be University)Plant Biotechnology Lab, Department of Botany, Faculty of Science, Dayalbagh Educational Institute (Deemed to be University)Plant Biotechnology Lab, Department of Botany, Faculty of Science, Dayalbagh Educational Institute (Deemed to be University)Abstract Piper longum, commonly known as long pepper, is highly valued for its bioactive alkaloid piperine, which has diverse pharmaceutical and culinary applications. In this study, we used high-throughput sequencing and de novo transcriptome assembly to analyze the transcriptomes of P. longum leaves, roots, and spikes. Our dataset consisted of 173,381 high-quality transcripts, with functional annotations highlighting key pathways involved in lysine biosynthesis and secondary metabolite production. We identified 8041 simple sequence repeats (SSRs), primarily trinucleotide repeats, adding valuable genetic markers. Additionally, we uncovered 21,235 transcription factors (TFs) across 65 families, including bHLH, NAC, and MYB, which are essential in regulating metabolism. KEGG pathway analysis categorized 4730 transcripts into 377 pathways, focusing on piperine biosynthesis, including phenylpropanoid and L-lysine metabolism. Our research identified 14 expressed genes encoding enzymes crucial for tropane, piperidine, and pyridine alkaloid biosynthesis, shedding light on piperine production mechanisms. High-performance liquid chromatography (HPLC) revealed varying piperine concentrations, with spikes showing the highest content, followed by roots and leaves. This comprehensive analysis of the P. longum transcriptome offers valuable insights into the genetics and regulatory networks underlying piperine biosynthesis, identifying molecular markers and genes that hold significant potential for enhancing the plant’s biological and pharmaceutical applications.https://doi.org/10.1038/s41598-025-87434-5Piper longumPiperineIlluminaBiosynthetic pathwaysTranscriptome analysisSecondary metabolites |
| spellingShingle | Mrinalini Prasad Shivangi Mathur Deeksha Singh Rajiv Ranjan De novo transcriptome profiling revealing genes involved in piperine biosynthetic pathway in Piper longum L Scientific Reports Piper longum Piperine Illumina Biosynthetic pathways Transcriptome analysis Secondary metabolites |
| title | De novo transcriptome profiling revealing genes involved in piperine biosynthetic pathway in Piper longum L |
| title_full | De novo transcriptome profiling revealing genes involved in piperine biosynthetic pathway in Piper longum L |
| title_fullStr | De novo transcriptome profiling revealing genes involved in piperine biosynthetic pathway in Piper longum L |
| title_full_unstemmed | De novo transcriptome profiling revealing genes involved in piperine biosynthetic pathway in Piper longum L |
| title_short | De novo transcriptome profiling revealing genes involved in piperine biosynthetic pathway in Piper longum L |
| title_sort | de novo transcriptome profiling revealing genes involved in piperine biosynthetic pathway in piper longum l |
| topic | Piper longum Piperine Illumina Biosynthetic pathways Transcriptome analysis Secondary metabolites |
| url | https://doi.org/10.1038/s41598-025-87434-5 |
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