Characterization of Human-Induced Neural Stem Cells and Derivatives following Transplantation into the Central Nervous System of a Nonhuman Primate and Rats
Neural stem cells (NSCs) and derivatives are potential cellular sources to treat neurological diseases. In the current study, we reprogrammed human peripheral blood mononuclear cells into induced NSCs (iNSCs) and inserted GFP gene into the AAVS1 site for graft tracing. Targeted integration of GFP do...
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| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2022/1396735 |
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| author | Mengjia Li Zhengbo Wang Tianqi Zheng Tianzhuang Huang Baoguo Liu Deqiang Han Sumei Liu Bochao Liu Mo Li Wei Si Y. Alex Zhang Yuyu Niu Zhiguo Chen |
| author_facet | Mengjia Li Zhengbo Wang Tianqi Zheng Tianzhuang Huang Baoguo Liu Deqiang Han Sumei Liu Bochao Liu Mo Li Wei Si Y. Alex Zhang Yuyu Niu Zhiguo Chen |
| author_sort | Mengjia Li |
| collection | DOAJ |
| description | Neural stem cells (NSCs) and derivatives are potential cellular sources to treat neurological diseases. In the current study, we reprogrammed human peripheral blood mononuclear cells into induced NSCs (iNSCs) and inserted GFP gene into the AAVS1 site for graft tracing. Targeted integration of GFP does not affect the proliferation and differentiation capacity of iNSCs. iNSC-GFP can be further differentiated into dopaminergic precursors (DAPs) and motor neuron precursors (MNPs), respectively. iNSCs were engrafted into the motor cortex and iNSC-DAPs into the striatum and substantia nigra (SN) of a nonhuman primate, respectively. The surviving iNSCs could respond to the microenvironment of the cortex and spontaneously differentiate into mature neurons that extended neurites. iNSC-DAPs survived well and matured into DA neurons following transplantation into the striatum and SN. iNSC-MNPs could also survive and turn into motor neurons after being engrafted into the spinal cord of rats. The results suggest that iNSCs and derivatives have a potential to be used for the treatment of neurological diseases. |
| format | Article |
| id | doaj-art-a7c059655d3a44ae852ca17d6ad59152 |
| institution | Kabale University |
| issn | 1687-9678 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-a7c059655d3a44ae852ca17d6ad591522025-02-03T05:57:38ZengWileyStem Cells International1687-96782022-01-01202210.1155/2022/1396735Characterization of Human-Induced Neural Stem Cells and Derivatives following Transplantation into the Central Nervous System of a Nonhuman Primate and RatsMengjia Li0Zhengbo Wang1Tianqi Zheng2Tianzhuang Huang3Baoguo Liu4Deqiang Han5Sumei Liu6Bochao Liu7Mo Li8Wei Si9Y. Alex Zhang10Yuyu Niu11Zhiguo Chen12Cell Therapy CenterState Key Laboratory of Primate Biomedical ResearchCell Therapy CenterState Key Laboratory of Primate Biomedical ResearchCell Therapy CenterCell Therapy CenterCell Therapy CenterCell Therapy CenterCell Therapy CenterState Key Laboratory of Primate Biomedical ResearchCell Therapy CenterState Key Laboratory of Primate Biomedical ResearchCell Therapy CenterNeural stem cells (NSCs) and derivatives are potential cellular sources to treat neurological diseases. In the current study, we reprogrammed human peripheral blood mononuclear cells into induced NSCs (iNSCs) and inserted GFP gene into the AAVS1 site for graft tracing. Targeted integration of GFP does not affect the proliferation and differentiation capacity of iNSCs. iNSC-GFP can be further differentiated into dopaminergic precursors (DAPs) and motor neuron precursors (MNPs), respectively. iNSCs were engrafted into the motor cortex and iNSC-DAPs into the striatum and substantia nigra (SN) of a nonhuman primate, respectively. The surviving iNSCs could respond to the microenvironment of the cortex and spontaneously differentiate into mature neurons that extended neurites. iNSC-DAPs survived well and matured into DA neurons following transplantation into the striatum and SN. iNSC-MNPs could also survive and turn into motor neurons after being engrafted into the spinal cord of rats. The results suggest that iNSCs and derivatives have a potential to be used for the treatment of neurological diseases.http://dx.doi.org/10.1155/2022/1396735 |
| spellingShingle | Mengjia Li Zhengbo Wang Tianqi Zheng Tianzhuang Huang Baoguo Liu Deqiang Han Sumei Liu Bochao Liu Mo Li Wei Si Y. Alex Zhang Yuyu Niu Zhiguo Chen Characterization of Human-Induced Neural Stem Cells and Derivatives following Transplantation into the Central Nervous System of a Nonhuman Primate and Rats Stem Cells International |
| title | Characterization of Human-Induced Neural Stem Cells and Derivatives following Transplantation into the Central Nervous System of a Nonhuman Primate and Rats |
| title_full | Characterization of Human-Induced Neural Stem Cells and Derivatives following Transplantation into the Central Nervous System of a Nonhuman Primate and Rats |
| title_fullStr | Characterization of Human-Induced Neural Stem Cells and Derivatives following Transplantation into the Central Nervous System of a Nonhuman Primate and Rats |
| title_full_unstemmed | Characterization of Human-Induced Neural Stem Cells and Derivatives following Transplantation into the Central Nervous System of a Nonhuman Primate and Rats |
| title_short | Characterization of Human-Induced Neural Stem Cells and Derivatives following Transplantation into the Central Nervous System of a Nonhuman Primate and Rats |
| title_sort | characterization of human induced neural stem cells and derivatives following transplantation into the central nervous system of a nonhuman primate and rats |
| url | http://dx.doi.org/10.1155/2022/1396735 |
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