Catalytically inactive Cas9 attenuates DNA end resection: A potential application for region-restricted random mutagenesis

Summary: Gene duplication followed by sequence diversification is a key driver of innovation in genome evolution. To mimic this process in genome engineering, a method for region-restricted mutagenesis is needed to selectively mutate one copy of a duplicated gene. Notably, regions flanking a double-...

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Bibliographic Details
Main Authors: Suchin Towa, Satoshi Okada, Takashi Ito
Format: Article
Language:English
Published: Elsevier 2025-06-01
Series:iScience
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2589004225009630
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